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Article Open Access

A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells

  • Authors:
    • Jian Zhang
    • Congxin Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3584-3592
    |
    Published online on: March 6, 2019
       https://doi.org/10.3892/mmr.2019.10012
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Abstract

Biological pacemakers that combine cell‑based and gene‑based therapies are a promising treatment for sick sinus syndrome or severe atrioventricular block. The current study aimed to induce differentiation of adipose tissue‑derived stem cells (ADSCs) into cardiac pacemaker cells through co‑expression of the transcription factors insulin gene enhancer binding protein 1 (ISL‑1) and T‑box18 (Tbx18). ADSCs were transfected with green fluorescent protein, ISL‑1, Tbx18 or ISL‑1+Tbx18 fluorescent protein lentiviral vectors, and subsequently co‑cultured with neonatal rat ventricular cardiomyocytes in vitro for 7 days. The potential for regulating the differentiation of ADSCs into pacemaker‑like cells was evaluated by cell morphology, beating rate, reverse transcription‑quantitative polymerase chain reaction, western blotting, immunofluorescence and electrophysiological activity. ADSCs were successfully transformed into spontaneously beating cells that exhibited a behavior similar to that of co‑cultured pacemaker cells. This effect was significantly increased in the combined ISL‑1 and Tbx18 group. These results provide a potential strategy for enriching the cardiac pacemaker cell population from ADSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J and Huang C: A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells. Mol Med Rep 19: 3584-3592, 2019.
APA
Zhang, J., & Huang, C. (2019). A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells. Molecular Medicine Reports, 19, 3584-3592. https://doi.org/10.3892/mmr.2019.10012
MLA
Zhang, J., Huang, C."A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells". Molecular Medicine Reports 19.5 (2019): 3584-3592.
Chicago
Zhang, J., Huang, C."A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells". Molecular Medicine Reports 19, no. 5 (2019): 3584-3592. https://doi.org/10.3892/mmr.2019.10012
Copy and paste a formatted citation
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Spandidos Publications style
Zhang J and Huang C: A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells. Mol Med Rep 19: 3584-3592, 2019.
APA
Zhang, J., & Huang, C. (2019). A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells. Molecular Medicine Reports, 19, 3584-3592. https://doi.org/10.3892/mmr.2019.10012
MLA
Zhang, J., Huang, C."A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells". Molecular Medicine Reports 19.5 (2019): 3584-3592.
Chicago
Zhang, J., Huang, C."A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells". Molecular Medicine Reports 19, no. 5 (2019): 3584-3592. https://doi.org/10.3892/mmr.2019.10012
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