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Article Open Access

miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1

  • Authors:
    • Bin Cao
    • Sheng Tan
    • Huijuan Tang
    • Yihui Chen
    • Peng Shu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210005, P.R. China, Department of Cardio‑Thoracic Surgery, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China, Department of Oncology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu 210005, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3604-3614
    |
    Published online on: March 14, 2019
       https://doi.org/10.3892/mmr.2019.10022
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Abstract

An increasing number of microRNA (miRNA) have been demonstrated to serve as molecular biomarkers for tumor cell progression. miR‑512‑5p was revealed as oncogenic regulator in several types of cancer. However, whether and how miR‑512‑5p regulates non‑small cell lung cancer (NSCLC) remains unclear. In the present study, the expression of miR‑512‑5p was detected in NSCLC tissues and cell lines. Then, the proliferation, migration, invasion and apoptosis in NSCLC A549 and H1299 cell lines were detected when miR‑512‑5p was overexpressed. Furthermore, the underlying mechanism was identified. The level of miR‑512‑5p was decreased in NSCLC tissues and in NSCLC cells compared with adjacent normal tissues and normal lung tissue cell lines. miR‑512‑5p mimics inhibited the cell proliferation, migration, invasion and induced apoptosis in A549 and H1299 cells. In addition, a luciferase reporter assay suggested that overexpression of miR‑512‑5p may decrease the expression of the E26 transformation specific‑1 (ETS1) gene; it was subsequently verified that downregulation of the ETS1 gene inhibited cell proliferation and migration and induced cell apoptosis in A549 and H1299 cells, and ETS1 small interfering RNA in the presence of an miR‑512‑5p inhibitor reversed the effect. The data described in the present study suggest that miR‑512‑5p may be a tumor suppressor and a potential treatment target in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Cao B, Tan S, Tang H, Chen Y and Shu P: miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1. Mol Med Rep 19: 3604-3614, 2019.
APA
Cao, B., Tan, S., Tang, H., Chen, Y., & Shu, P. (2019). miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1. Molecular Medicine Reports, 19, 3604-3614. https://doi.org/10.3892/mmr.2019.10022
MLA
Cao, B., Tan, S., Tang, H., Chen, Y., Shu, P."miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1". Molecular Medicine Reports 19.5 (2019): 3604-3614.
Chicago
Cao, B., Tan, S., Tang, H., Chen, Y., Shu, P."miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1". Molecular Medicine Reports 19, no. 5 (2019): 3604-3614. https://doi.org/10.3892/mmr.2019.10022
Copy and paste a formatted citation
x
Spandidos Publications style
Cao B, Tan S, Tang H, Chen Y and Shu P: miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1. Mol Med Rep 19: 3604-3614, 2019.
APA
Cao, B., Tan, S., Tang, H., Chen, Y., & Shu, P. (2019). miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1. Molecular Medicine Reports, 19, 3604-3614. https://doi.org/10.3892/mmr.2019.10022
MLA
Cao, B., Tan, S., Tang, H., Chen, Y., Shu, P."miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1". Molecular Medicine Reports 19.5 (2019): 3604-3614.
Chicago
Cao, B., Tan, S., Tang, H., Chen, Y., Shu, P."miR‑512‑5p suppresses proliferation, migration and invasion, and induces apoptosis in non‑small cell lung cancer cells by targeting ETS1". Molecular Medicine Reports 19, no. 5 (2019): 3604-3614. https://doi.org/10.3892/mmr.2019.10022
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