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Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress

  • Authors:
    • Xiaohui Fu
    • Yimiao Feng
    • Bingyi Shao
    • Yanzhen Zhang
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    Affiliations: Department of General Dentistry, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Orthodontics, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Operative Dentistry and Endodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, P.R. China
    Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3649-3657
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    Published online on: March 14, 2019
       https://doi.org/10.3892/mmr.2019.10027
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Abstract

Periodontal ligament stem cells (PDLSCs) are promising stem cells sources for regenerative medicine, particularly clinical periodontal ligament repair. It is critical to maintain high quality and a large quantity of PDLSCs for clinical usage. However, how PDLSCs respond to environmental stimuli, including reactive oxygen species (ROS), is poorly understood. The aim of the present study was to investigate how PDLSCs react to oxidative stress and the underlying mechanisms. Hydrogen peroxide‑induced oxidative stress was used to mimic a ROS increase in rat PDLSCs. The expression levels of Creb were detected under oxidative stress to examine the role that Creb serves in PDLSCs under oxidative stress. The present results demonstrated that the expression of Creb was reduced in a dose‑dependent manner in response to the H2O2 stimulus. Overexpressing Creb significantly reduced the ROS levels and protein expression levels of apoptotic genes in PDLSCs. The phosphorylation of the ERK pathway is indispensable in the activation of Creb‑induced protection. Our results revealed a protective role of Creb in ROS‑induced apoptosis, and validated the ERK/Creb/apoptosis regulator Bcl‑2 pathway works as an anti‑apoptotic signaling in PDLSCs. These findings will facilitate the in vitro culturing of PDLSCs for clinical usage and promote stem cell based therapy for periodontal tissue regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Fu X, Feng Y, Shao B and Zhang Y: Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress. Mol Med Rep 19: 3649-3657, 2019.
APA
Fu, X., Feng, Y., Shao, B., & Zhang, Y. (2019). Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress. Molecular Medicine Reports, 19, 3649-3657. https://doi.org/10.3892/mmr.2019.10027
MLA
Fu, X., Feng, Y., Shao, B., Zhang, Y."Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress". Molecular Medicine Reports 19.5 (2019): 3649-3657.
Chicago
Fu, X., Feng, Y., Shao, B., Zhang, Y."Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress". Molecular Medicine Reports 19, no. 5 (2019): 3649-3657. https://doi.org/10.3892/mmr.2019.10027
Copy and paste a formatted citation
x
Spandidos Publications style
Fu X, Feng Y, Shao B and Zhang Y: Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress. Mol Med Rep 19: 3649-3657, 2019.
APA
Fu, X., Feng, Y., Shao, B., & Zhang, Y. (2019). Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress. Molecular Medicine Reports, 19, 3649-3657. https://doi.org/10.3892/mmr.2019.10027
MLA
Fu, X., Feng, Y., Shao, B., Zhang, Y."Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress". Molecular Medicine Reports 19.5 (2019): 3649-3657.
Chicago
Fu, X., Feng, Y., Shao, B., Zhang, Y."Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress". Molecular Medicine Reports 19, no. 5 (2019): 3649-3657. https://doi.org/10.3892/mmr.2019.10027
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