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Article

Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5

  • Authors:
    • Fengying Gao
    • Rong Chen
    • Yaofeng Xi
    • Qiujian Zhao
    • Haifeng Gao
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Burn, Baoji Center Hospital, Baoji, Shaanxi 721008, P.R. China, Clinical Laboratory, Baoji Center Hospital, Baoji, Shaanxi 721008, P.R. China
  • Pages: 3756-3766
    |
    Published online on: March 14, 2019
       https://doi.org/10.3892/mmr.2019.10028
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Abstract

The present study aimed to identify the involvement of the dysregulation of the metastasis‑associated lung adenocarcinoma transcript‑1 (MALAT1)/microRNA (miR)‑214/Toll‑like receptor (TLR)5 signaling pathway in the development of post‑burn sepsis. THP‑1 cells were used in the present study, in addition to 8‑10 week‑old mice. ELISA analysis was performed to examine the expression levels of inflammation‑associated factors. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blotting were performed to analyze the influence of burns or burns with infection on the production of MALAT1, miR‑214 and TLR5. Commonly‑used software and a luciferase assay was used to confirm the target gene of miR‑214. RT‑qPCR analysis and western blotting were performed to elucidate the effects of lipopolysaccharide (LPS), miR‑214 and MALAT1 on the expression of miR‑214, TLR5, tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑10. Burn injury increased TLR5, TNF‑α, IL‑6 and IL‑10 expression levels, which were abolished by treatment with MALAT1. miR‑214 directly targeted TLR5 by binding to the TLR5 3' untranslated region (ITR), and the luciferase activity of the wild‑type, and not the mutant, TLR5 3'UTR was reduced following transfection with miR‑214. In cells not treated with LPS, MALAT1 and anti‑miR‑214 significantly enhanced TLR5, TNF‑α, IL‑6 and IL‑10 expression, and repressed miR‑214 production; whereas, miR‑214 and MALAT1 short hairpin (sh)RNA decreased TLR5, TNF‑α, IL‑6 and IL‑10 expression levels, and increased miR‑214 expression. In cells treated with LPS, LPS reduced miR‑214 expression and increased TLR5, TNF‑α, IL‑6 and IL‑10 expression compared with LPS‑untreated cells, and the effects of MALAT1, anti‑miR‑214, miR‑214 and MALAT1 shRNA on TLR5, TNF‑α, IL‑6 and IL‑10 were the same as in LPS‑untreated cell. The results of the present study indicated the association between the dysregulation of MALAT1/miR‑214/TLR5 and the risk of post‑burn sepsis.
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1 

Murray CK: Infections in burns. J Trauma. 62:S732007. View Article : Google Scholar : PubMed/NCBI

2 

Pruitt BA Jr, McManus AT, Kim SH and Goodwin CW: Burn wound infections: Current status. World J Surg. 22:135–145. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Appelgren P, Bjornhagen V, Bragderyd K, Jonsson CE and Ransjo U: A prospective study of infections in burn patients. Burns. 28:39–46. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Branski LK, Al-Mousawi A, Rivero H, Jeschke MG, Sanford AP and Herndon DN: Emerging infections in burns. Surgical Infections. 10:389–397. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Williams FN, Herndon DN, Hawkins HK, Lee JO, Cox RA, Kulp GA, Finnerty CC, Chinkes DL and Jeschke MG: The leading causes of death after burn injury in a single pediatric burn center. Crit Care. 13:R1832009. View Article : Google Scholar : PubMed/NCBI

6 

Mann EA, Baun MM, Meininger JC and Wade CE: Comparison of mortality associated with sepsis in the burn, trauma, and general intensive care unit patient: A systematic review of the literature. Shock. 37:4–16. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, Maeda N, Oyama R, Ravasi T, Lenhard B, Wells C, et al RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group), : The transcriptional landscape of the mammalian genome. Science. 309:1559–1563. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Kim VN, Han J and Siomi MC: Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol. 10:126–139. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Mercer TR, Dinger ME and Mattick JS: Long non-coding RNAs: Insights into functions. Nat Rev Genet. 10:155–159. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Fabian MR, Sonenberg N and Filipowicz W: Regulation of mRNA translation and stability by microRNAs. Annu Rev Biochem. 79:351–379. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Gutschner T, Hammerle M and Diederichs S: MALAT1-A paradigm for long noncoding RNA function in cancer. J Mol Med (Berl). 91:791–801. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Yan B, Tao ZF, Li XM, Zhang H, Yao J and Jiang Q: Aberrant expression of long noncoding RNAs in early diabetic retinopathy. Invest Ophthalmol Vis Sci. 55:941–951. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Puthanveetil P, Chen S, Feng B, Gautam A and Chakrabarti S: Long non-coding RNA MALAT1 regulates hyperglycaemia induced inflammatory process in the endothelial cells. J Cell Mol Med. 19:1418–1425. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Silva SC, Baggio-Zappia GL, Brunialti MK, Assuncao MS, Azevedo LC, Machado FR and Salomao R: Evaluation of toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomes. Braz J Med Biol Res. 47:384–393. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Zhuo Y, Zeng Q, Zhang P, Li G, Xie Q and Cheng Y: Functional polymorphism of lncRNA MALAT1 contributes to pulmonary arterial hypertension susceptibility in Chinese people. Clin Chem Lab Med. 55:38–46. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Lahiri R, Derwa Y, Bashir Z, Giles E, Torrance HD, Owen HC, O'Dwyer MJ, O'Brien A, Stagg AJ, Bhattacharya S, et al: Systemic inflammatory response syndrome after major abdominal surgery predicted by early upregulation of TLR4 and TLR5. Ann Surg. 263:1028–1037. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Liu Y, Zhou Q, Wang Y, Liu Z, Dong M, Wang Y, Li X and Hu D: Negative pressure wound therapy decreases mortality in a murine model of burn-wound sepsis involving pseudomonas aeruginosa infection. PLoS One. 9:e904942014. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Iwagaki A, Porro M and Pollack M: Influence of synthetic antiendotoxin peptides on lipopolysaccharide (LPS) recognition and LPS-induced proinflammatory cytokine responses by cells expressing membrane-bound CD14. Infect Immun. 68:1655–1663. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Zhao G, Su Z, Song D, Mao Y and Mao X: The long noncoding RNA MALAT1 regulates the lipopolysaccharide-induced inflammatory response through its interaction with NF-kappaB. FEBS Lett. 590:2884–2895. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Hu L, Wu Y, Tan D, Meng H, Wang K, Bai Y and Yang K: Up-regulation of long noncoding RNA MALAT1 contributes to proliferation and metastasis in esophageal squamous cell carcinoma. J Exp Clin Cancer Res. 34:72015. View Article : Google Scholar : PubMed/NCBI

22 

Li LM, Hou DX, Guo YL, Yang JW, Liu Y, Zhang CY and Zen K: Role of microRNA-214-targeting phosphatase and tensin homolog in advanced glycation end product-induced apoptosis delay in monocytes. J Immunol. 186:2552–2560. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Jindra PT, Bagley J, Godwin JG and Iacomini J: Costimulation- dependent expression of microRNA-214 increases the ability of T cells to proliferate by targeting Pten. J Immunol. 185:990–997. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Zhao L, Liu YW, Yang T, Gan L, Yang N, Dai SS and He F: The mutual regulation between miR-214 and A2AR signaling plays an important role in inflammatory response. Cell Signal. 27:2026–2034. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Gonzales JN, Gorshkov B, Varn MN, Zemskova MA, Zemskov EA, Sridhar S, Lucas R and Verin AD: Protective effect of adenosine receptors against lipopolysaccharide-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol. 306:L497–L507. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Honko AN and Mizel SB: Effects of flagellin on innate and adaptive immunity. Immunol Res. 33:83–101. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Moors MA, Li L and Mizel SB: Activation of interleukin-1 receptor-associated kinase by gram-negative flagellin. Infect Immun. 69:4424–4429. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Gohda J, Matsumura T and Inoue J: Cutting edge: TNFR- associated factor (TRAF) 6 is essential for MyD88-dependent pathway but not toll/IL-1 receptor domain-containing adaptor-inducing IFN-beta (TRIF)-dependent pathway in TLR signaling. J Immunol. 173:2913–2917. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Means TK, Hayashi F, Smith KD, Aderem A and Luster AD: The Toll-like receptor 5 stimulus bacterial flagellin induces maturation and chemokine production in human dendritic cells. J Immunol. 170:5165–5175. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Mizel SB and Bates JT: Flagellin as an adjuvant: Cellular mechanisms and potential. J Immunol. 185:5677–5682. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Salamone GV, Petracca Y, Fuxman Bass JI, Rumbo M, Nahmod KA, Gabelloni ML, Vermeulen ME, Matteo MJ, Geffner JR and Trevani AS: Flagellin delays spontaneous human neutrophil apoptosis. Lab Invest. 90:1049–1059. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Bone RC, Sibbald WJ and Sprung CL: The ACCP-SCCM consensus conference on sepsis and organ failure. Chest. 101:1481–1483. 1992. View Article : Google Scholar : PubMed/NCBI

33 

Boomer JS, To K, Chang KC, Takasu O, Osborne DF, Walton AH, Bricker TL, Jarman SD II, Kreisel D, Krupnick AS, et al: Immunosuppression in patients who die of sepsis and multiple organ failure. JAMA. 306:2594–2605. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Salomao R, Brunialti MK, Rapozo MM, Baggio-Zappia GL, Galanos C and Freudenberg M: Bacterial sensing, cell signaling, and modulation of the immune response during sepsis. Shock. 38:227–242. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Janeway CA Jr and Medzhitov R: Innate immune recognition. Annu Rev Immunol. 20:197–216. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Vijay-Kumar M, Sanders CJ, Taylor RT, Kumar A, Aitken JD, Sitaraman SV, Neish AS, Uematsu S, Akira S, Williams IR and Gewirtz AT: Deletion of TLR5 results in spontaneous colitis in mice. J Clin Invest. 117:3909–3921. 2007.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gao F, Chen R, Xi Y, Zhao Q and Gao H: Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5. Mol Med Rep 19: 3756-3766, 2019.
APA
Gao, F., Chen, R., Xi, Y., Zhao, Q., & Gao, H. (2019). Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5. Molecular Medicine Reports, 19, 3756-3766. https://doi.org/10.3892/mmr.2019.10028
MLA
Gao, F., Chen, R., Xi, Y., Zhao, Q., Gao, H."Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5". Molecular Medicine Reports 19.5 (2019): 3756-3766.
Chicago
Gao, F., Chen, R., Xi, Y., Zhao, Q., Gao, H."Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5". Molecular Medicine Reports 19, no. 5 (2019): 3756-3766. https://doi.org/10.3892/mmr.2019.10028
Copy and paste a formatted citation
x
Spandidos Publications style
Gao F, Chen R, Xi Y, Zhao Q and Gao H: Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5. Mol Med Rep 19: 3756-3766, 2019.
APA
Gao, F., Chen, R., Xi, Y., Zhao, Q., & Gao, H. (2019). Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5. Molecular Medicine Reports, 19, 3756-3766. https://doi.org/10.3892/mmr.2019.10028
MLA
Gao, F., Chen, R., Xi, Y., Zhao, Q., Gao, H."Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5". Molecular Medicine Reports 19.5 (2019): 3756-3766.
Chicago
Gao, F., Chen, R., Xi, Y., Zhao, Q., Gao, H."Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5". Molecular Medicine Reports 19, no. 5 (2019): 3756-3766. https://doi.org/10.3892/mmr.2019.10028
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