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Article

Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice

  • Authors:
    • Shao‑Qin Ge
    • Yin‑Ling Wu
    • Jin‑Liang Zhu
    • Yuan Tian
    • Shu‑Song Wang
    • Zhang‑Quan Gao
  • View Affiliations / Copyright

    Affiliations: The Institute of Traditional Chinese Medicine of Hebei University Health Science Centre, Baoding, Hebei 071002, P.R. China, The Medical Comprehensive Laboratory Center of Hebei University Health Science Centre, Baoding, Hebei 071002, P.R. China, The Centre for Reproductive Medicine of Peking University Third Hospital, Beijing 100191, P.R. China, Key Laboratory of Family Planning and Healthy Birth, National Health and Family Planning Commission Hebei Research Institute for Family Planning, Shijiazhuang, Hebei 050071, P.R. China
  • Pages: 4401-4406
    |
    Published online on: March 21, 2019
       https://doi.org/10.3892/mmr.2019.10072
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Abstract

The objective of the present study was to investigate the effects of for chlorfenuron (FCF) interference with the septin protein on early stage embryos in mice. The 1‑cell embryos were collected and divided into an FCF interference group and a control group. The FCF interference group was cultured in FCF media and the control group was cultured in dimethyl sulphoxide media at 37˚C with 5% CO2 until the desired phase was achieved. Septin2 protein expression was detected using immunofluorescence and western blot analysis. Blastocyst α‑tubulin was stained by immunofluorescence to observe the alterations in spindles and microtubules. The rate of early embryo development into blastocysts was significantly reduced following FCF treatment (P<0.05). In the control group, septin2 was observed with a confocal microscope; septin2 was expressed in embryos at all stages and mainly in the blastomeres from the 2‑cell stage onwards, with the expression concentrated in the nuclei of the blastomeres as identified by strong fluorescence. In the FCF interference group, septin2 was weakly expressed in the nuclei of blastomeres at the 2‑ and 4‑cell stages, and in the granulated blastomeres at the 4‑ and 8‑cell stages. Expression was barely observed in and following the morula. Granulation was observed starting from the 4‑ and 8‑cell stages. Compared with the control group, the FCF interference group exhibited irregular microtubules, abnormal spindle morphology and disordered chromosome arrangement in the blastocysts. The septin2 protein was expressed throughout the early stage embryo from the 2‑cell stage to the blastocyst and localized in the nuclei of blastomeres. When the septin protein experienced interference by the FCF inhibitor, septin2 protein expression was reduced, which simultaneously resulted in abnormal embryonic development, uneven cytoplasmic division, various sizes and a reduced number of blastomeres, granulation in the blastomeres, disordered blastocyst microtubule distribution, spindle shape alterations and an abnormality of chromosome arrangement.
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Copy and paste a formatted citation
Spandidos Publications style
Ge SQ, Wu YL, Zhu JL, Tian Y, Wang SS and Gao ZQ: Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice. Mol Med Rep 19: 4401-4406, 2019.
APA
Ge, S., Wu, Y., Zhu, J., Tian, Y., Wang, S., & Gao, Z. (2019). Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice. Molecular Medicine Reports, 19, 4401-4406. https://doi.org/10.3892/mmr.2019.10072
MLA
Ge, S., Wu, Y., Zhu, J., Tian, Y., Wang, S., Gao, Z."Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice". Molecular Medicine Reports 19.5 (2019): 4401-4406.
Chicago
Ge, S., Wu, Y., Zhu, J., Tian, Y., Wang, S., Gao, Z."Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice". Molecular Medicine Reports 19, no. 5 (2019): 4401-4406. https://doi.org/10.3892/mmr.2019.10072
Copy and paste a formatted citation
x
Spandidos Publications style
Ge SQ, Wu YL, Zhu JL, Tian Y, Wang SS and Gao ZQ: Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice. Mol Med Rep 19: 4401-4406, 2019.
APA
Ge, S., Wu, Y., Zhu, J., Tian, Y., Wang, S., & Gao, Z. (2019). Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice. Molecular Medicine Reports, 19, 4401-4406. https://doi.org/10.3892/mmr.2019.10072
MLA
Ge, S., Wu, Y., Zhu, J., Tian, Y., Wang, S., Gao, Z."Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice". Molecular Medicine Reports 19.5 (2019): 4401-4406.
Chicago
Ge, S., Wu, Y., Zhu, J., Tian, Y., Wang, S., Gao, Z."Preliminary investigation of the effects of an FCF inhibitor interference with Septin in the early stage embryos in mice". Molecular Medicine Reports 19, no. 5 (2019): 4401-4406. https://doi.org/10.3892/mmr.2019.10072
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