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Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species

  • Authors:
    • Ying Pi
    • Haifeng Liang
    • Qiang Yu
    • Yukun Yin
    • Haixia Xu
    • Yutian Lei
    • Zhongyu Han
    • Jing Tian
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Human Anatomy, Basic Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Pi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4129-4136
    |
    Published online on: March 22, 2019
       https://doi.org/10.3892/mmr.2019.10079
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Abstract

Bone homeostasis is a dynamic balance maintained by bone formation and resorption. An increase in the number and activity of osteoclasts leads to excessive bone resorption, which in turn results in bone disease, including osteoporosis. Therefore, inhibiting the differentiation and activity of osteoclasts is important for maintaining bone mass. Several studies have revealed that the use of a low‑frequency pulsed electromagnetic field (PEMF) is an effective method to treat osteoporosis. However, its exact mechanism remains to be fully clarified. Therefore, the present study was designed to examine the effects that PEMF exerts on receptor activator of nuclear factor‑κB ligand (RANKL)‑induced osteoclastogenesis and intracellular reactive oxygen species (ROS) production in RAW264.7 cells. The viability of cells was determined using a Cell Counting Kit‑8 assay, and gene and protein expression were investigated via reverse transcription‑quantitative polymerase chain reaction and western blot analyses. Furthermore, microscopy was performed to detect the levels of intracellular ROS and tartrate‑resistant acid phosphatase (TRAP). Following the culture of RAW264.7 cells with RANKL (50 ng/ml) for 4 days (3 h/day) under PEMF (75 Hz, 1 mt) exposure, it was observed that PEMF had an inhibitory effect on RANKL‑induced osteoclastic differentiation. Multinucleated osteoclast formation, the activity of TRAP and the expression of osteoclastogenesis‑associated genes, including cathepsin K, nuclear factor of activated T cells cytoplasmic 1 and TRAP, were significantly reduced by PEMF. Furthermore, PEMF effectively decreased the generation of intracellular ROS during osteoclastic differentiation. In addition, the results demonstrated that ROS are the key factor in osteoclast differentiation and formation. Reducing intracellular ROS with diphenylene‑iodonium chloride significantly inhibited RANKL‑induced osteoclast differentiation. Taken together, the results of the present study demonstrated that PEMF may inhibit RANKL‑induced osteoclastogenesis by scavenging intracellular ROS. These results may provide the groundwork for future PEMF clinical applications in osteoclast‑associated bone disease.
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1 

Lemaire V, Tobin FL, Greller LD, Cho CR and Suva LJ: Modeling the interactions between osteoblast and osteoclast activities in bone remodeling. J Theor Biol. 229:293–309. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Bloemen V, Schoenmaker T, de Vries TJ and Everts V: Direct cell-cell contact between periodontal ligament fibroblasts and osteoclast precursors synergistically increases the expression of genes related to osteoclastogenesis. J Cell Physiol. 222:565–573. 2010.PubMed/NCBI

3 

Boyle WJ, Simonet WS and Lacey DL: Osteoclast differentiation and activation. Nature. 423:337–342. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Reid IR: Short-term and long-term effects of osteoporosis therapies. Nat Rev Endocrinol. 11:418–428. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Harvey N, Dennison E and Cooper C: Osteoporosis: Impact on health and economics. Nat Rev Rheumatol. 6:99–105. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Adami HO, Persson I, Hoover R, Schairer C and Bergkvist L: Risk of cancer in women receiving hormone replacement therapy. Int J Cancer. 44:833–839. 1989. View Article : Google Scholar : PubMed/NCBI

7 

Lewiecki EM: Treatment of osteoporosis with denosumab. Maturitas. 66:182–186. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Jing D, Li F, Jiang M, Cai J, Wu Y, Xie K, Wu X, Tang C, Liu J, Guo W, et al: Pulsed electromagnetic fields improve bone microstructure and strength in ovariectomized rats through a Wnt/Lrp5/β-catenin signaling-associated mechanism. PLoS One. 8:e793772013. View Article : Google Scholar : PubMed/NCBI

9 

Hannemann PF, Mommers EH, Schots JP, Brink PR and Poeze M: The effects of low-intensity pulsed ultrasound and pulsed electromagnetic fields bone growth stimulation in acute fractures: A systematic review and meta-analysis of randomized controlled trials. Arch Orthop Trauma Surg. 134:1093–1106. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Chang K, Chang WH, Tsai MT and Shih C: Pulsed electromagnetic fields accelerate apoptotic rate in osteoclasts. Connect Tissue Res. 47:222–228. 2006. View Article : Google Scholar : PubMed/NCBI

11 

He Z, Selvamurugan N, Warshaw J and Partridge NC: Pulsed electromagnetic fields inhibit human osteoclast formation and gene expression via osteoblasts. Bone. 106:194–203. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Zhou J, Liao Y, Zeng Y, Xie H, Fu C and Li N: Effect of intervention initiation timing of pulsed electromagnetic field on ovariectomy-induced osteoporosis in rats. Bioelectromagnetics. 38:456–465. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Assiotis A, Sachinis NP and Chalidis BE: Pulsed electromagnetic fields for the treatment of tibial delayed unions and nonunions. A prospective clinical study and review of the literature. J Orthop Surg Res. 7:242012. View Article : Google Scholar : PubMed/NCBI

14 

Kim H, Kim IY, Lee SY and Jeong D: Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts. FEBS Lett. 580:5661–5665. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Domazetovic V, Marcucci G, Iantomasi T, Brandi ML and Vincenzini MT: Oxidative stress in bone remodeling: Role of antioxidants. Clin Cases Miner Bone Metab. 14:209–216. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Davies KJ: Oxidative stress: The paradox of aerobic life. Biochem Soc Symp. 61:1–31. 1995. View Article : Google Scholar : PubMed/NCBI

17 

Forman HJ, Fukuto JM and Torres M: Redox signaling: Thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers. Am J Physiol Cell Physiol. 287:C246–C256. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Lander HM: An essential role for free radicals and derived species in signal transduction. FASEB J. 11:118–124. 1997. View Article : Google Scholar : PubMed/NCBI

19 

Callaway DA and Jiang JX: Reactive oxygen species and oxidative stress in osteoclastogenesis, skeletal aging and bone diseases. J Bone Miner Metab. 33:359–370. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Lean JM, Davies JT, Fuller K, Jagger CJ, Kirstein B, Partington GA, Urry ZL and Chambers TJ: A crucial role for thiol antioxidants in estrogen-deficiency bone loss. J Clin Invest. 112:915–923. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Moon HJ, Ko WK, Han SW, Kim DS, Hwang YS, Park HK and Kwon IK: Antioxidants, like coenzyme Q10, selenite, and curcumin, inhibited osteoclast differentiation by suppressing reactive oxygen species generation. Biochem Biophys Res Commun. 418:247–253. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Moon HJ, Kim SE, Yun YP, Hwang YS, Bang JB, Park JH and Kwon IK: Simvastatin inhibits osteoclast differentiation by scavenging reactive oxygen species. Exp Mol Med. 43:605–612. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Garrett IR, Boyce BF, Oreffo RO, Bonewald L, Poser J and Mundy GR: Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo. J Clin Invest. 85:632–639. 1990. View Article : Google Scholar : PubMed/NCBI

24 

Ishii KA, Fumoto T, Iwai K, Takeshita S, Ito M, Shimohata N, Aburatani H, Taketani S, Lelliott CJ, Vidal-Puig A and Ikeda K: Coordination of PGC-1beta and iron uptake in mitochondrial biogenesis and osteoclast activation. Nat Med. 15:259–266. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Bartell SM, Kim HN, Ambrogini E, Han L, Iyer S, Serra Ucer S, Rabinovitch P, Jilka RL, Weinstein RS, Zhao H, et al: FoxO proteins restrain osteoclastogenesis and bone resorption by attenuating H2O2 accumulation. Nat Commun. 5:37732014. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Lee SH, Ding Y, Yan XT, Kim YH and Jang HD: Scopoletin and scopolin isolated from Artemisia iwayomogi suppress differentiation of osteoclastic macrophage RAW 264.7 cells by scavenging reactive oxygen species. J Nat Prod. 76:615–620. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Teitelbaum SL and Ross FP: Genetic regulation of osteoclast development and function. Nat Rev Genet. 4:638–649. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Tucker JJ, Cirone JM, Morris TR, Nuss CA, Huegel J, Waldorff EI, Zhang N, Ryaby JT and Soslowsky LJ: Pulsed electromagnetic field therapy improves tendon-to-bone healing in a rat rotator cuff repair model. J Orthop Res. 35:902–909. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Osti L, Buono AD and Maffulli N: Pulsed electromagnetic fields after rotator cuff repair: A randomized, controlled study. Orthopedics. 38:e223–e228. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Veronesi F, Torricelli P, Giavaresi G, Sartori M, Cavani F, Setti S, Cadossi M, Ongaro A and Fini M: In vivo effect of two different pulsed electromagnetic field frequencies on osteoarthritis. J Orthop Res. 32:677–685. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Hartig M, Joos U and Wiesmann HP: Capacitively coupled electric fields accelerate proliferation of osteoblast-like primary cells and increase bone extracellular matrix formation in vitro. Eur Biophys J. 29:499–506. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Lohmann CH, Schwartz Z, Liu Y, Guerkov H, Dean DD, Simon B and Boyan BD: Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects differentiation and local factor production. J Orthop Res. 18:637–646. 2000. View Article : Google Scholar : PubMed/NCBI

34 

He J, Zhang Y, Chen J, Zheng S, Huang H and Dong X: Effects of pulsed electromagnetic fields on the expression of NFATc1 and CAII in mouse osteoclast-like cells. Aging Clin Exp Res. 27:13–19. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Jing D, Cai J, Wu Y, Shen G, Li F, Xu Q, Xie K, Tang C, Liu J, Guo W, et al: Pulsed electromagnetic fields partially preserve bone mass, microarchitecture, and strength by promoting bone formation in hindlimb-suspended rats. J Bone Miner Res. 29:2250–2261. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Wang P, Liu J, Yang Y, Zhai M, Shao X, Yan Z, Zhang X, Wu Y, Cao L, Sui B, et al: Differential intensity-dependent effects of pulsed electromagnetic fields on RANKL-induced osteoclast formation, apoptosis, and bone resorbing ability in RAW264.7 cells. Bioelectromagnetics. 38:602–612. 2017. View Article : Google Scholar : PubMed/NCBI

37 

Negishi-Koga T and Takayanagi H: Ca2+-NFATc1 signaling is an essential axis of osteoclast differentiation. Immunol Rev. 231:241–256. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Pall ML: Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects. J Cell Mol Med. 17:958–965. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Cornacchione M, Pellegrini M, Fassina L, Mognaschi ME, Di Siena S, Gimmelli R, Ambrosino P, Soldovieri MV, Taglialatela M, Gianfrilli D, et al: β-Adrenergic response is counteracted by extremely-low-frequency pulsed electromagnetic fields in beating cardiomyocytes. J Mol Cell Cardiol. 98:146–158. 2016. View Article : Google Scholar : PubMed/NCBI

40 

Lee NK, Choi YG, Baik JY, Han SY, Jeong DW, Bae YS, Kim N and Lee SY: A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation. Blood. 106:852–859. 2005. View Article : Google Scholar : PubMed/NCBI

41 

Decuypere JP, Monaco G, Missiaen L, De Smedt H, Parys JB and Bultynck G: IP(3) receptors, mitochondria, and Ca signaling: Implications for aging. J Aging Res. 2011:9201782011. View Article : Google Scholar : PubMed/NCBI

42 

Kim H, Lee YD, Kim HJ, Lee ZH and Kim HH: SOD2 and Sirt3 control osteoclastogenesis by regulating mitochondrial ROS. J Bone Miner Res. 32:397–406. 2017. View Article : Google Scholar : PubMed/NCBI

43 

Huh JE, Shin JH, Jang ES, Park SJ, Park DR, Ko R, Seo DH, Kim HS, Lee SH, Choi Y, et al: Sirtuin 3 (SIRT3) maintains bone homeostasis by regulating AMPK-PGC-1β axis in mice. Sci Rep. 6:225112016. View Article : Google Scholar : PubMed/NCBI

44 

Sendur OF, Turan Y, Tastaban E and Serter M: Antioxidant status in patients with osteoporosis: A controlled study. Joint Bone Spine. 76:514–518. 2009. View Article : Google Scholar : PubMed/NCBI

45 

Chen Y, Sun J, Dou C, Li N, Kang F, Wang Y, Cao Z, Yang X and Dong S: Alliin attenuated RANKL-induced osteoclastogenesis by scavenging reactive oxygen species through inhibiting Nox1. Int J Mol Sci. 17(pii): E15162016. View Article : Google Scholar : PubMed/NCBI

46 

Rao LG, Krishnadev N, Banasikowska K and Rao AV: Lycopene I-effect on osteoclasts: Lycopene inhibits basal and parathyroid hormone-stimulated osteoclast formation and mineral resorption mediated by reactive oxygen species in rat bone marrow cultures. J Med Food. 6:69–78. 2003. View Article : Google Scholar : PubMed/NCBI

47 

Falone S, Grossi MR, Cinque B, D'Angelo B, Tettamanti E, Cimini A, Di Ilio C and Amicarelli F: Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells. Int J Biochem Cell Biol. 39:2093–2106. 2007. View Article : Google Scholar : PubMed/NCBI

48 

Simkó M: Cell type specific redox status is responsible for diverse electromagnetic field effects. Curr Med Chem. 14:1141–1152. 2007. View Article : Google Scholar : PubMed/NCBI

49 

Mannerling AC, Simko M, Mild KH and Mattsson MO: Effects of 50-Hz magnetic field exposure on superoxide radical anion formation and HSP70 induction in human K562 cells. Radiat Environ Biophys. 49:731–741. 2010. View Article : Google Scholar : PubMed/NCBI

50 

Osera C, Fassina L, Amadio M, Venturini L, Buoso E, Magenes G, Govoni S, Ricevuti G and Pascale A: Cytoprotective response induced by electromagnetic stimulation on SH-SY5Y human neuroblastoma cell line. Tissue Eng Part A. 17:2573–2582. 2011. View Article : Google Scholar : PubMed/NCBI

51 

Ehnert S, Fentz AK, Schreiner A, Birk J, Wilbrand B, Ziegler P, Reumann MK, Wang H, Falldorf K and Nussler AK: Extremely low frequency pulsed electromagnetic fields cause antioxidative defense mechanisms in human osteoblasts via induction of •O2− and H2O2. Sci Rep. 7:145442017. View Article : Google Scholar : PubMed/NCBI

52 

Falone S, Marchesi N, Osera C, Fassina L, Comincini S, Amadio M and Pascale A: Pulsed electromagnetic field (PEMF) prevents pro-oxidant effects of H2O2 in SK-N-BE(2) human neuroblastoma cells. Int J Radiat Biol. 92:281–286. 2016. View Article : Google Scholar : PubMed/NCBI

53 

Suda T, Takahashi F and Takahashi N: Bone effects of vitamin D-discrepancies between in vivo and in vitro studies. Arch Biochem Biophys. 523:22–29. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pi Y, Liang H, Yu Q, Yin Y, Xu H, Lei Y, Han Z and Tian J: Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species. Mol Med Rep 19: 4129-4136, 2019.
APA
Pi, Y., Liang, H., Yu, Q., Yin, Y., Xu, H., Lei, Y. ... Tian, J. (2019). Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species. Molecular Medicine Reports, 19, 4129-4136. https://doi.org/10.3892/mmr.2019.10079
MLA
Pi, Y., Liang, H., Yu, Q., Yin, Y., Xu, H., Lei, Y., Han, Z., Tian, J."Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species". Molecular Medicine Reports 19.5 (2019): 4129-4136.
Chicago
Pi, Y., Liang, H., Yu, Q., Yin, Y., Xu, H., Lei, Y., Han, Z., Tian, J."Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species". Molecular Medicine Reports 19, no. 5 (2019): 4129-4136. https://doi.org/10.3892/mmr.2019.10079
Copy and paste a formatted citation
x
Spandidos Publications style
Pi Y, Liang H, Yu Q, Yin Y, Xu H, Lei Y, Han Z and Tian J: Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species. Mol Med Rep 19: 4129-4136, 2019.
APA
Pi, Y., Liang, H., Yu, Q., Yin, Y., Xu, H., Lei, Y. ... Tian, J. (2019). Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species. Molecular Medicine Reports, 19, 4129-4136. https://doi.org/10.3892/mmr.2019.10079
MLA
Pi, Y., Liang, H., Yu, Q., Yin, Y., Xu, H., Lei, Y., Han, Z., Tian, J."Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species". Molecular Medicine Reports 19.5 (2019): 4129-4136.
Chicago
Pi, Y., Liang, H., Yu, Q., Yin, Y., Xu, H., Lei, Y., Han, Z., Tian, J."Low‑frequency pulsed electromagnetic field inhibits RANKL‑induced osteoclastic differentiation in RAW264.7 cells by scavenging reactive oxygen species". Molecular Medicine Reports 19, no. 5 (2019): 4129-4136. https://doi.org/10.3892/mmr.2019.10079
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