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Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells

  • Authors:
    • Feng Yu
    • Ke Ye
    • Yunfeng Hu
    • Jincheng Li
    • Yonglei An
    • Dawei Qu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Automotive Simulation and Control, Jilin University, Changchun, Jilin 130011, P.R. China, Department of Gastrointestinal Colorectal Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4326-4334
    |
    Published online on: March 26, 2019
       https://doi.org/10.3892/mmr.2019.10086
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Abstract

Long‑term exposure to vehicle exhaust gas may lead to various age‑associated disorders, including cardiovascular disease and cancer. Polycyclic aromatic hydrocarbons (PAHs) belong to an important class of carcinogens, which are released into the environment by vehicles and are detectable at high levels in Chinese urban areas. However, whether vehicle exhaust gas (EG), and in particular the PAHs derived from EG, are able to induce cell senescence remains unclear. In the present study, vehicle EG and pure PAHs were used as pollution sources to investigate the effects of long‑term exposure to PAH on the cellular processes occurring in mouse lung fibroblast cells (mLFCs). Using cell proliferation and apoptosis assays, it was demonstrated that benzopyrene (BaP) suppressed the proliferation of mLFCs, and benzanthracene (BaA) and BaP induced cell apoptosis. Molecular analysis suggested that long‑term exposure to BaA and BaP was able to increase the protein expression levels of p53, p21 and the apoptotic factors involved in the caspase cascade, including caspase‑3 and ‑9. Notably, the present study suggested that PAH exposure was able to promote cell senescence in mLFCs by activating the ATM serine/threonine kinase/H2A histone family member X pathway. The present study may provide novel insights into the underlying mechanism of vehicle EG and PAHs in promoting the development of age‑associated diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Yu F, Ye K, Hu Y, Li J, An Y and Qu D: Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells. Mol Med Rep 19: 4326-4334, 2019.
APA
Yu, F., Ye, K., Hu, Y., Li, J., An, Y., & Qu, D. (2019). Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells. Molecular Medicine Reports, 19, 4326-4334. https://doi.org/10.3892/mmr.2019.10086
MLA
Yu, F., Ye, K., Hu, Y., Li, J., An, Y., Qu, D."Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells". Molecular Medicine Reports 19.5 (2019): 4326-4334.
Chicago
Yu, F., Ye, K., Hu, Y., Li, J., An, Y., Qu, D."Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells". Molecular Medicine Reports 19, no. 5 (2019): 4326-4334. https://doi.org/10.3892/mmr.2019.10086
Copy and paste a formatted citation
x
Spandidos Publications style
Yu F, Ye K, Hu Y, Li J, An Y and Qu D: Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells. Mol Med Rep 19: 4326-4334, 2019.
APA
Yu, F., Ye, K., Hu, Y., Li, J., An, Y., & Qu, D. (2019). Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells. Molecular Medicine Reports, 19, 4326-4334. https://doi.org/10.3892/mmr.2019.10086
MLA
Yu, F., Ye, K., Hu, Y., Li, J., An, Y., Qu, D."Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells". Molecular Medicine Reports 19.5 (2019): 4326-4334.
Chicago
Yu, F., Ye, K., Hu, Y., Li, J., An, Y., Qu, D."Exposure to polycyclic aromatic hydrocarbons derived from vehicle exhaust gas induces premature senescence in mouse lung fibroblast cells". Molecular Medicine Reports 19, no. 5 (2019): 4326-4334. https://doi.org/10.3892/mmr.2019.10086
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