Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2019 Volume 19 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2019 Volume 19 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration

  • Authors:
    • Dingyun Li
    • Tao Zhang
    • Jiajun Lai
    • Jian Zhang
    • Ting Wang
    • Yafei Ling
    • Shengquan He
    • Zhiwei Hu
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Yue Bei People's Hospital, Shaoguan, Guangdong 512026, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4213-4221
    |
    Published online on: March 27, 2019
       https://doi.org/10.3892/mmr.2019.10090
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the function of microRNA‑25 (miR‑25) in human colon cancer cell viability and migration in addition to the underlying possible mechanisms. miR‑25 expression was upregulated in patients with colon cancer compared with the control group. Reverse transcription‑quantitative polymerase chain reaction and gene chip technology were used to analyze the alterations of miR‑25 in patients with colon cancer. Cell viability and cell migration were analyzed using MTT and wound healing assays, respectively, apoptosis was analyzed using flow cytometry, and western blot analysis was conducted to determine the protein expression of ataxin‑3 (ATXN3), apoptosis regulator Bax (Bax) and cyclin D1. Overexpression of miR‑25 increased cell viability and migration, decreased apoptosis, decreased caspase‑3/9 activity level in addition to decreased Bax protein expression, and increased cyclin D1 protein expression in colon cancer cells. Furthermore, miR‑25 was demonstrated to target ATXN3 and suppress ATXN3 protein expression. Downregulation of miR‑25 induced apoptosis of colon cancer cells via increased expression ATXN3. Small interfering‑ATXN3 inhibited the anti‑cancer effects of miR‑25 downregulation in colon cancer. Collectively, the present results demonstrated that miR‑25 promoted human colon cancer cell viability and migration by regulating ATXN3 expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Guo J, Tao W, Tang D and Zhang J: Th17/regulatory T cell imbalance in sepsis patients with multiple organ dysfunction syndrome: attenuated by high-volume hemofiltration. Int J Artif Organs. 40:607–614. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Zivkovic AR, Tourelle KM, Brenner T, Weigand MA, Hofer S and Schmidt K: Reduced serum cholinesterase activity indicates splenic modulation of the sterile inflammation. J Surg Res. 220:275–283. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Wang X, Luo B, Lu Y, Pang D, Zheng J, Mo J, Huang H and Feng J: The triggering receptor expressed by myeloid cells-1 activates TLR4-MyD88-NF-κB-dependent signaling to aggravate ventilation-induced lung inflammation and injury in mice. Cell Tissue Res. 374:137–148. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Li JZ, Wang ZL, Xu WH, Li Q, Gao L and Wang ZM: MicroRNA-495 regulates migration and invasion in prostate cancer cells via targeting Akt and mTOR signaling. Cancer Invest. 34:181–188. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Wang H, Li XT, Wu C, Wu ZW, Li YY, Yang TQ, Chen GL, Xie XS, Huang YL, Du ZW and Zhou YX: miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro. Onco Targets Ther. 8:3211–3218. 2015.PubMed/NCBI

6 

Zhang D, Sun G, Zhang H, Tian J and Li Y: Long non-coding RNA ANRIL indicates a poor prognosis of cervical cancer and promotes carcinogenesis via PI3K/Akt pathways. Biomed Pharmacother. 85:511–516. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Yao C, Shi X, Zhang Z, Zhou S, Qian T, Wang Y, Ding F, Gu X and Yu B: Hypoxia-induced upregulation of miR-132 promotes schwann cell migration after sciatic nerve injury by targeting PRKAG3. Mol Neurobiol. 53:5129–5139. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Leinders M, Üçeyler N, Pritchard RA, Sommer C and Sorkin LS: Increased miR-132-3p expression is associated with chronic neuropathic pain. Exp Neurol. 283:276–286. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Xu T, Pang Q, Wang Y and Yan X: Betulinic acid induces apoptosis by regulating PI3K/Akt signaling and mitochondrial pathways in human cervical cancer cells. Int J Mol Med. 40:1669–1678. 2017.PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Yadav N and Chandra H: Suppression of inflammatory and infection responses in lung macrophages by eucalyptus oil and its constituent 1,8-cineole: Role of pattern recognition receptors TREM-1 and NLRP3, the MAP kinase regulator MKP-1, and NFκB. PLoS One. 12:e01882322017. View Article : Google Scholar : PubMed/NCBI

12 

van Kampen JGM, van Hooij O, Jansen CF, Smit FP, van Noort PI, Schultz I, Schaapveld RQJ, Schalken JA and Verhaegh GW: miRNA-520f Reverses epithelial-to-mesenchymal transition by targeting ADAM9 and TGFBR2. Cancer Res. 77:2008–2017. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Liu XJ, Zheng XP, Zhang R, Guo YL and Wang JH: Combinatorial effects of miR-20a and miR-29b on neuronal apoptosis induced by spinal cord injury. Int J Clin Exp Pathol. 8:3811–3818. 2015.PubMed/NCBI

14 

He J, Qi H, Chen F and Cao C: MicroRNA-25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a. Oncol Lett. 14:6097–6102. 2017.PubMed/NCBI

15 

Li W, Lu M, Zhang Y, Xia D, Chen Z, Wang L, Yin N and Wang Z: Puerarin attenuates the daunorubicin-induced apoptosis of H9c2 cells by activating the PI3K/Akt signaling pathway via the inhibition of Ca2+ influx. Int J Mol Med. 40:1889–1894. 2017.PubMed/NCBI

16 

Qiu ZK, Zhong DS, He JL, Liu X, Chen JS and Nie H: The anxiolytic-like effects of puerarin are associated with the changes of monoaminergic neurotransmitters and biosynthesis of allopregnanolone in the brain. Metab Brain Dis. 33:167–175. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Feng T, Zheng L, Liu F, Xu X, Mao S, Wang X, Liu J, Lu Y, Zhao W, Yu X and Tang W: Growth factor progranulin promotes tumorigenesis of cervical cancer via PI3K/Akt/mTOR signaling pathway. Oncotarget. 7:58381–58395. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Huang F, Zhang L, Long Z, Chen Z, Hou X, Wang C, Peng H, Wang J, Li J, Duan R, et al: miR-25 alleviates polyQ-mediated cytotoxicity by silencing ATXN3. FEBS Lett. 588:4791–4798. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Bahadori M, Baharara J and Amini E: Anticancer properties of chrysin on colon cancer cells, in vitro and in vivo with modulation of caspase-3, −9, Bax and Sall4. Iran J Biotechnol. 14:177–184. 2016. View Article : Google Scholar : PubMed/NCBI

20 

LeBlanc H, Lawrence D, Varfolomeev E, Totpal K, Morlan J, Schow P, Fong S, Schwall R, Sinicropi D and Ashkenazi A: Tumor-cell resistance to death receptor-induced apoptosis through mutational inactivation of the proapoptotic Bcl-2 homolog Bax. Nat Med. 8:274–281. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Zhang H, Zuo Z, Lu X, Wang L, Wang H and Zhu Z: MiR-25 regulates apoptosis by targeting Bim in human ovarian cancer. Oncol Rep. 27:594–598. 2012.PubMed/NCBI

22 

Wang J, Li XM, Bai Z, Chi BX, Wei Y and Chen X: Curcumol induces cell cycle arrest in colon cancer cells via reactive oxygen species and Akt/GSK3β/cyclin D1 pathway. J Ethnopharmacol. 210:1–9. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Li C, Peng W, Song X, Wang Q and Wang W: Anticancer effect of icaritin inhibits cell growth of colon cancer through reactive oxygen species, Bcl-2 and cyclin D1/E signaling. Oncol Lett. 12:3537–3542. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Huo J, Zhang Y, Li R, Wang Y, Wu J and Zhang D: Upregulated MicroRNA-25 mediates the migration of melanoma cells by targeting DKK3 through the WNT/β-catenin pathway. Int J Mol Sci. 17(pii): E11242016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li D, Zhang T, Lai J, Zhang J, Wang T, Ling Y, He S and Hu Z: MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration. Mol Med Rep 19: 4213-4221, 2019.
APA
Li, D., Zhang, T., Lai, J., Zhang, J., Wang, T., Ling, Y. ... Hu, Z. (2019). MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration. Molecular Medicine Reports, 19, 4213-4221. https://doi.org/10.3892/mmr.2019.10090
MLA
Li, D., Zhang, T., Lai, J., Zhang, J., Wang, T., Ling, Y., He, S., Hu, Z."MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration". Molecular Medicine Reports 19.5 (2019): 4213-4221.
Chicago
Li, D., Zhang, T., Lai, J., Zhang, J., Wang, T., Ling, Y., He, S., Hu, Z."MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration". Molecular Medicine Reports 19, no. 5 (2019): 4213-4221. https://doi.org/10.3892/mmr.2019.10090
Copy and paste a formatted citation
x
Spandidos Publications style
Li D, Zhang T, Lai J, Zhang J, Wang T, Ling Y, He S and Hu Z: MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration. Mol Med Rep 19: 4213-4221, 2019.
APA
Li, D., Zhang, T., Lai, J., Zhang, J., Wang, T., Ling, Y. ... Hu, Z. (2019). MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration. Molecular Medicine Reports, 19, 4213-4221. https://doi.org/10.3892/mmr.2019.10090
MLA
Li, D., Zhang, T., Lai, J., Zhang, J., Wang, T., Ling, Y., He, S., Hu, Z."MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration". Molecular Medicine Reports 19.5 (2019): 4213-4221.
Chicago
Li, D., Zhang, T., Lai, J., Zhang, J., Wang, T., Ling, Y., He, S., Hu, Z."MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration". Molecular Medicine Reports 19, no. 5 (2019): 4213-4221. https://doi.org/10.3892/mmr.2019.10090
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team