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Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury

  • Authors:
    • Xinghui Wang
    • Hualong Zhao
    • Jun Ni
    • Jiacheng Pan
    • Hao Hua
    • Yaxian Wang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co‑innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, P.R. China, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, Jiangsu 224051, P.R. China, Department of Rehabilitation, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Medicine, Xinglin College, Nantong University, Nantong, Jiangsu 226001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4377-4387
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    Published online on: March 28, 2019
       https://doi.org/10.3892/mmr.2019.10102
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Abstract

Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) is a molecular biological method used to assess gene expression characterized by high simplicity, effectiveness, specificity and sensitivity. The selection of a suitable reference gene for normalization is critical for the accuracy of quantitative results. Peripheral nerve injury is a common clinical disorder that affects multiple tissues and organs, including peripheral nerves, neurons and the innervated muscles. Numerous genes are differentially expressed in skeletal muscles during muscle denervation and reinnervation following peripheral nerve injury. The identification of a suitable reference gene in innervated muscles following nerve injury may improve the understanding of the alterations in gene expression in the processes of peripheral nerve repair and regeneration. Therefore, in the present study, by using a rat sciatic nerve crush model, the expression levels of various housekeeping genes were examined. In particular, the expression levels of 13 housekeeping genes, including 18S ribosomal RNA, actin β, ankyrin repeat domain 27, cyclophilin A, GAPDH, hypoxanthine phosphoribosyltransferase 1 (HPRT1), mitochondrial ribosomal protein L10, phosphoglycerate kinase 1, RPTOR independent companion of mammalian target of rapamycin complex 2, TATA‑box binding protein, ubiquitin C, UBX domain protein 11 and tyrosine 3‑monooxygenase/tryptophan 5‑monooxygenase activation protein ζ, were investigated in gastrocnemius muscles. The geNorm and NormFinder analyses suggested that the expression level of HPRT1 was particularly stable in gastrocnemius muscles following rat sciatic nerve crush injury. Therefore, HPRT1 may be used as a reference gene for the normalization of gene expression data generated by RT‑qPCR.
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View References

1 

Bustin SA: Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J Mol Endocrinol. 29:23–39. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Seol D, Choe H, Zheng H, Jang K, Ramakrishnan PS, Lim TH and Martin JA: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc. BMC Res Notes. 4:1622011. View Article : Google Scholar : PubMed/NCBI

3 

Andersen CL, Jensen JL and Orntoft TF: Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64:5245–5250. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Eisenberg E and Levanon EY: Human housekeeping genes are compact. Trends Genet. 19:362–365. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Zhu J, He F, Hu S and Yu J: On the nature of human housekeeping genes. Trends Genet. 24:481–484. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A and Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:RESEARCH00342002. View Article : Google Scholar : PubMed/NCBI

7 

Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A and Heinen E: Housekeeping genes as internal standards: Use and limits. J Biotechnol. 75:291–295. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Schmittgen TD and Zakrajsek BA: Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods. 46:69–81. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Li R, Liu Z, Pan Y, Chen L, Zhang Z and Lu L: Peripheral nerve injuries treatment: A systematic review. Cell Biochem Biophys. 68:449–54. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Gu X, Ding F and Williams DF: Neural tissue engineering options for peripheral nerve regeneration. Biomaterials. 35:6143–6156. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Yi S, Zhang H, Gong L, Wu J, Zha G, Zhou S, Gu X and Yu B: Deep sequencing and bioinformatic analysis of lesioned sciatic nerves after crush injury. PLoS One. 10:e01434912015. View Article : Google Scholar : PubMed/NCBI

12 

Gong L, Wu J, Zhou S, Wang Y, Qin J, Yu B, Gu X and Yao C: Global analysis of transcriptome in dorsal root ganglia following peripheral nerve injury in rats. Biochem Biophys Res Commun. 478:206–212. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Wang Y, Shan Q, Meng Y, Pan J and Yi S: Mrpl10 and Tbp are suitable reference genes for peripheral nerve crush injury. Int J Mol Sci. 18:E2632017. View Article : Google Scholar : PubMed/NCBI

14 

Lien SC, Cederna PS and Kuzon WM Jr: Optimizing skeletal muscle reinnervation with nerve transfer. Hand Clin. 24445–454. (vii)2008. View Article : Google Scholar : PubMed/NCBI

15 

Irintchev A and Wernig A: Denervation and reinnervation of muscle: Physiological effects. Eur Arch Otorhinolaryngol. S28–S30. 1994.PubMed/NCBI

16 

Martínez-Martos JM, Carrera-González MP, Sánchez-Agesta R, García MJ and Ramírez-Expósito MJ: Kidney aminopeptidase activities are related to renal damage in experimental breast cancer. J Clin Mol Med. 1:2018. View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–428. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Bangaru ML, Park F, Hudmon A, McCallum BJ and Hogan HQ: Quantification of gene expression after painful nerve injury: Validation of optimal reference genes. J Mol Neurosci. 46:497–504. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Bonefeld BE, Elfving B and Wegener G: Reference genes for normalization: A study of rat brain tissue. Synapse. 62:302–309. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Gambarotta G, Ronchi G, Friard O, Galletta P, Perroteau I and Geuna S: Identification and validation of suitable housekeeping genes for normalizing quantitative real-time PCR assays in injured peripheral nerves. PLoS One. 9:e1056012014. View Article : Google Scholar : PubMed/NCBI

21 

Peinnequin A, Mouret C, Birot O, Alonso A, Mathieu J, Clarençon D, Agay D, Chancerelle Y and Multon E: Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green. BMC Immunol. 5:32004. View Article : Google Scholar : PubMed/NCBI

22 

Martínez-Beamonte R, Navarro MA, Larraga A, Strunk M, Barranquero C, Acín S, Guzman MA, Iñigo P and Osada J: Selection of reference genes for gene expression studies in rats. J Biotechnol. 151:325–334. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Carvalho RF, Castan EP, Coelho CA, Lopes FS, Almeida FL, Michelin A, de Souza RW, Araújo JP Jr, Cicogna AC and Dal Pai-Silva M: Heart failure increases atrogin-1 and MuRF1 gene expression in skeletal muscle with fiber type-specific atrophy. J Mol Histol. 41:81–87. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Rho HW, Lee BC, Choi ES, Choi IJ, Lee YS and Goh SH: Identification of valid reference genes for gene expression studies of human stomach cancer by reverse transcription-qPCR. BMC Cancer. 10:2402010. View Article : Google Scholar : PubMed/NCBI

25 

Li P, Waters RE, Redfern SI, Zhang M, Mao L, Annex BH and Yan Z: Oxidative phenotype protects myofibers from pathological insults induced by chronic heart failure in mice. Am J Pathol. 170:599–608. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Latres E, Amini AR, Amini AA, Griffiths J, Martin FJ, Wei Y, Lin HC, Yancopoulos GD and Glass DJ: Insulin-like growth factor-1 (IGF-1) inversely regulates atrophy-induced genes via the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway. J Biol Chem. 280:2737–2744. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Yoshimura K, Asato H, Cederna PS, Urbanchek MG and Kuzon WM: The effect of reinnervation on force production and power output in skeletal muscle. J Surg Res. 81:201–208. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Kamath S, Venkatanarasimha N, Walsh MA and Hughes PM: MRI appearance of muscle denervation. Skeletal Radiol. 37:397–404. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Jaweed MM, Herbison GM and Ditunno JF: Denervation and reinnervation of fast and slow muscles. A histochemical study in rats. J Histochem Cytochem. 23:808–827. 1975. View Article : Google Scholar : PubMed/NCBI

30 

Carlson BM: The Biology of long-term denervated skeletal muscle. Eur J Transl Myol. 24:32932014. View Article : Google Scholar : PubMed/NCBI

31 

Willand MP, Nguyen MA, Borschel GH and Gordon T: Electrical stimulation to promote peripheral nerve regeneration. Neurorehabil Neural Repair. 30:490–496. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Batt J, Bain J, Goncalves J, Michalski B, Plant P, Fahnestock M and Woodgett J: Differential gene expression profiling of short and long term denervated muscle. FASEB J. 20:115–117. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Nicolino S, Panetto A, Raimondo S, Gambarotta G, Guzzini M, Fornaro M, Battiston B, Tos P, Geuna S and Perroteau I: Denervation and reinnervation of adult skeletal muscle modulate mRNA expression of neuregulin-1 and ErbB receptors. Microsurgery. 29:464–472. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Sun H, Qiu J, Chen Y, Yu M, Ding F and Gu X: Proteomic and bioinformatic analysis of differentially expressed proteins in denervated skeletal muscle. Int J Mol Med. 33:1586–1596. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Weng J, Zhang P, Yin X and Jiang B: The Whole transcriptome involved in denervated muscle atrophy following peripheral nerve injury. Front Mol Neurosci. 11:692018. View Article : Google Scholar : PubMed/NCBI

36 

Raffaello A, Laveder P, Romualdi C, Bean C, Toniolo L, Germinario E, Megighian A, Danieli-Betto D, Reggiani C and Lanfranchi G: Denervation in murine fast-twitch muscle: Short-term physiological changes and temporal expression profiling. Physiol Genomics. 25:60–74. 2006. View Article : Google Scholar : PubMed/NCBI

37 

Hsieh CH, Jeng SF, Wu CJ, Lu TH, Yang JC, Chen YC, Lin CJ and Rau CS: Altered expression of the microRNAS and their potential target genes in the soleus muscle after peripheral denervation and reinnervation in rats. J Trauma. 70:472–480. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Jeng SF, Rau CS, Liliang PC, Wu CJ, Lu TH, Chen YC, Lin CJ and Hsieh CH: Profiling muscle-specific microRNA expression after peripheral denervation and reinnervation in a rat model. J Neurotrauma. 26:2345–2353. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Li G, Li QS, Li WB, Wei J, Chang WK, Chen Z, Qiao HY, Jia YW, Tian JH and Liang BS: MIRNA targeted signaling pathway in the early stage of denervated fast and slow muscle atrophy. Neural Regen Res. 11:1293–1303. 2016. View Article : Google Scholar : PubMed/NCBI

40 

Sellner LN and Turbett GR: The presence of a pseudogene may affect the use of HPRT as an endogenous mRNA control in RT-PCR. Mol Cell Probes. 10:481–483. 1996. View Article : Google Scholar : PubMed/NCBI

41 

Nelissen K, Smeets K, Mulder M, Hendriks JJ and Ameloot M: Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR. J Neurosci Methods. 187:78–83. 2010. View Article : Google Scholar : PubMed/NCBI

42 

Solano ME, Thiele K, Kowal MK and Arck PC: Identification of suitable reference genes in the mouse placenta. Placenta. 39:7–15. 2016. View Article : Google Scholar : PubMed/NCBI

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Spandidos Publications style
Wang X, Zhao H, Ni J, Pan J, Hua H and Wang Y: Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury. Mol Med Rep 19: 4377-4387, 2019.
APA
Wang, X., Zhao, H., Ni, J., Pan, J., Hua, H., & Wang, Y. (2019). Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury. Molecular Medicine Reports, 19, 4377-4387. https://doi.org/10.3892/mmr.2019.10102
MLA
Wang, X., Zhao, H., Ni, J., Pan, J., Hua, H., Wang, Y."Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury". Molecular Medicine Reports 19.5 (2019): 4377-4387.
Chicago
Wang, X., Zhao, H., Ni, J., Pan, J., Hua, H., Wang, Y."Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury". Molecular Medicine Reports 19, no. 5 (2019): 4377-4387. https://doi.org/10.3892/mmr.2019.10102
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhao H, Ni J, Pan J, Hua H and Wang Y: Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury. Mol Med Rep 19: 4377-4387, 2019.
APA
Wang, X., Zhao, H., Ni, J., Pan, J., Hua, H., & Wang, Y. (2019). Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury. Molecular Medicine Reports, 19, 4377-4387. https://doi.org/10.3892/mmr.2019.10102
MLA
Wang, X., Zhao, H., Ni, J., Pan, J., Hua, H., Wang, Y."Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury". Molecular Medicine Reports 19.5 (2019): 4377-4387.
Chicago
Wang, X., Zhao, H., Ni, J., Pan, J., Hua, H., Wang, Y."Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury". Molecular Medicine Reports 19, no. 5 (2019): 4377-4387. https://doi.org/10.3892/mmr.2019.10102
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