CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma

Ma,Chao; Nie,Xing‑Guo; Wang,Yan‑Li; Liu,Xiang‑Hua; Liang,Xue; Zhou,Qing‑Lan; Wu,Da‑Peng

doi:10.3892/mmr.2019.10104

May-2019 Volume 19 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-2019 Volume 19 Issue 5

Full Size Image

Article Open Access

CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma

Authors:
- Chao Ma
- Xing‑Guo Nie
- Yan‑Li Wang
- Xiang‑Hua Liu
- Xue Liang
- Qing‑Lan Zhou
- Da‑Peng Wu
View Affiliations / Copyright

Affiliations: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China, Department of Operating Theatre, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China, Department of Central Sterile Supply, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China

Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 4205-4212
|
Published online on: March 28, 2019

https://doi.org/10.3892/mmr.2019.10104
Expand metrics +

Abstract

CBX3, namely chromobox protein homolog 3, a member of the heterochomatin protein 1 (HP1) family, has been shown to be associated with the tumorigenesis of various types of cancer. The aim of the present study was to assess the biological role and the clinicopathological importance of CBX3 in osteosarcoma. The Oncomine database was utilized to determine the CBX3 expression in sarcoma patients. A retrospective cohort study was conducted to evaluate the prognostic value of CBX3 expression. In addition, correlations between the clinicopathological features of the osteosarcoma patients and CBX3 expression were assessed and involved recurrence, distant metastasis, lymph node metastasis, response to chemotherapy, pathological differentiation, clinical stage, anatomic location, tumor size and age. To investigate the function of CBX3 in osteosarcoma, a small interfering RNA for CBX3 was designed and this was used for the transfection of osteosarcoma MG63 cells. Then, the effects of CBX3 on proliferation, cell cycle distribution and apoptosis of osteosarcoma cells were investigated via CCK‑8 assay and cell cycle assay and cell apoptosis analysis, respectively. Based on our findings, upregulation of CBX3 expression was noted both in osteosarcoma and also other sarcoma types, which included pleomorphic liposarcoma, myxofibrosarcoma, myxoid/round cell liposarcoma and dedifferentiated liposarcoma. In addition, based on the retrospective cohort study, CBX3 expression was associated with the disease‑free survival (DFS) and overall survival (OS) of the osteosarcoma patients and a large tumor size, high distant metastasis rate and high clinical stage rate. In addition, the proliferation ability was blocked by the knockdown of CBX3 through the application of CBX3 siRNA, and CBX3 knockdown also led to increased apoptosis and cell cycle arrest at G0 and G1 phases in osteosarcoma cells. CBX3 is highly expressed in human osteosarcoma tissues. Meanwhile, high CBX3 is a predictor of the poor prognosis of osteosarcoma patients. To conclude, the growth of osteosarcoma can be promoted by CBX3, which may be used as an independent potential prognostic biomarker for patients suffering from osteosarcoma.

View Figures

View References

1	Zhang GY, Zhang JF, Hu XM, Luo ZP and Ma YZ: Clinical significance of long non-coding RNA EWSAT1 as a novel prognostic biomarker in osteosarcoma. Eur Rev Med Pharmacol Sci. 21:5337–5341. 2017.PubMed/NCBI
2	Fan J, Mei J, Zhang MZ, Yuan F, Li SZ, Yu GR, Chen LH, Tang Q and Xian CJ: Clinicopathological significance of glucose transporter protein-1 overexpression in human osteosarcoma. Oncol Lett. 14:2439–2445. 2017. View Article : Google Scholar : PubMed/NCBI
3	Yoshida A, Fujiwara T, Uotani K, Morita T, Kiyono M, Yokoo S, Hasei J, Nakata E, Kunisada T and Ozaki T: Clinical and functional significance of intracellular and extracellular microRNA-25-3p in osteosarcoma. Acta Med Okayama. 72:165–174. 2018.PubMed/NCBI
4	Longhi A, Errani C, De Paolis M, Mercuri M and Bacci G: Primary bone osteosarcoma in the pediatric age: State of the art. Cancer Treat Rev. 32:423–436. 2006. View Article : Google Scholar : PubMed/NCBI
5	Chou AJ, Geller DS and Gorlick R: Therapy for osteosarcoma: Where do we go from here? Paediatr Drugs. 10:315–327. 2008. View Article : Google Scholar : PubMed/NCBI
6	Fan Y, Li H, Liang X and Xiang Z: CBX3 promotes colon cancer cell proliferation by CDK6 kinase-independent function during cell cycle. Oncotarget. 8:19934–19946. 2017.PubMed/NCBI
7	Canzio D, Larson A and Narlikar GJ: Mechanisms of functional promiscuity by HP1 proteins. Trends Cell Biol. 24:377–386. 2014. View Article : Google Scholar : PubMed/NCBI
8	Esteller M: Cancer epigenomics: DNA methylomes and histone-modification maps. Nat Rev Genet. 8:286–298. 2007. View Article : Google Scholar : PubMed/NCBI
9	Chen LY, Cheng CS, Qu C, Wang P, Chen H, Meng ZQ and Chen Z: CBX3 promotes proliferation and regulates glycolysis via suppressing FBP1 in pancreatic cancer. Biochem Biophys Res Commun. 500:691–697. 2018. View Article : Google Scholar : PubMed/NCBI
10	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
11	Dialynas GK, Vitalini MW and Wallrath LL: Linking heterochromatin protein 1 (HP1) to cancer progression. Mutat Res. 647:13–20. 2008. View Article : Google Scholar : PubMed/NCBI
12	Zhang H, Chen W, Fu X, Su X and Yang A: CBX3 promotes tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma. Gene. 654:49–56. 2018. View Article : Google Scholar : PubMed/NCBI
13	Chang SC, Lai YC, Chen YC, Wang NK, Wang WS and Lai JI: CBX3/heterochromatin protein 1 gamma is significantly upregulated in patients with non-small cell lung cancer. Asia Pac J Clin Oncol. Nov 10–2017.(Epub ahead of print). doi: 10.1111/ajco.12820.
14	Slezak J, Truong M, Huang W and Jarrard D: HP1γ expression is elevated in prostate cancer and is superior to Gleason score as a predictor of biochemical recurrence after radical prostatectomy. BMC Cancer. 13:1482013. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles