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Article Open Access

Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway

Retraction in: /10.3892/mmr.2025.13549
  • Authors:
    • Min Chen
    • Jin‑Min Wang
    • Dong Wang
    • Rong Wu
    • Hong‑Wei Hou
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Nanjing Second Hospital, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210009, P.R. China, Department of General Surgery, Southeast University Medical School, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4841-4851
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    Published online on: April 3, 2019
       https://doi.org/10.3892/mmr.2019.10117
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Abstract

Anastomotic fibrosis is highly likely to lead to reoperation in Crohn's disease (CD) patients. Triptolide (TPL) is considered to have anti‑inflammatory and antifibrotic effects in a variety of autoimmune diseases, including CD. The present study aimed to investigate the effects of TPL on fibroblasts from strictured ileocolonic anastomosis of patients with CD and its underlying mechanism. Primary fibroblasts were obtained from strictured anastomosis tissue (SAT) samples and matched anastomosis‑adjacent normal tissue (NT) samples which were collected from 10 CD patients who underwent reoperation because of anastomotic stricture. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to measure miR‑16‑1 and heat shock protein 70 (HSP70) levels. Western blotting was conducted to determine expression of HSP70, collagen I (Col‑I), collagen III (Col‑III) and α‑smooth muscle actin (α‑SMA) proteins. Agomir‑16‑1 and antagomir‑16‑1 were used to up and downregulate the expression of miR‑16‑1, respectively. Small interfering RNA (siRNA) was employed to inhibit the expression of HSP70. A wound healing assay was performed to measure the migration of fibroblasts. Cell proliferation was evaluated by MTT and 5‑bromo‑2‑deoxyrudidine assays. Cell apoptosis was determined by caspase‑3 activity and TUNEL assays. The results demonstrated that the levels of Col‑I, Col‑III and α‑SMA were all significantly upregulated in SAT compared with NT. miR‑16‑1 levels in the SAT group were significantly compared with the NT group; conversely, the expression levels of HSP70 mRNA and protein in the SAT group were significantly lower compared with the NT group. Next, fibroblasts were treated with TPL to examine its effect on the miR‑16‑1/HSP70 pathway. The results demonstrated that the elevated expression of miR‑16‑1 in the SAT group was effectively inhibited by TPL treatment. Compared with the NT group, both the mRNA and protein levels of HSP70 were significantly downregulated in the SAT group cells, while TPL exhibited a strong promoting effect on HSP70 synthesis. Furthermore, upregulation of miR‑16‑1 reversed the effect of TPL on the miR‑16‑1/HSP70 pathway in fibroblasts from SAT. Overexpression of miR‑16‑1 significantly reversed the inhibitory effects of TPL treatment on migration, proliferation and extracellular matrix (ECM)‑associated protein expression of fibroblasts from SAT. Finally, downregulation of miR‑16‑1 caused similar effects to the fibroblasts as the TPL treatment; however, the inhibitory effects on cell biological functions induced by antagomir‑16‑1 were all significantly reversed by HSP70 silencing. The present findings indicated that TPL may be a potential therapeutic option for postoperative anastomosis fibrosis of patients with CD. The miR‑16‑1/HSP70 pathway had a substantial role in the inhibitory effects of TPL on migration, proliferation and ECM synthesis rate of fibroblasts from strictured anastomosis tissues.
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View References

1 

Yamamoto T and Keighley MR: Long-term results of strictureplasty for ileocolonic anastomotic recurrence in Crohn's disease. J Gastrointest Surg. 3:555–560. 1999. View Article : Google Scholar : PubMed/NCBI

2 

De Cruz P, Kamm MA, Hamilton AL, Ritchie KJ, Krejany EO, Gorelik A, Liew D, Prideaux L, Lawrance IC, Andrews JM, et al: Crohn's disease management after intestinal resection: A randomised trial. Lancet. 385:1406–1417. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Ananthakrishnan AN: Surgery for Crohn's disease: Look harder, act faster. Lancet. 385:1370–1371. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Froehlich F, Juillerat P, Mottet C, Felley C, Vader JP, Burnand B, Gonvers JJ and Michetti P: Obstructive fibrostenotic Crohn's disease. Digestion. 71:29–30. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Legnani PE and Kornbluth A: Therapeutic options in the management of strictures in Crohn's disease. Gastrointest Endosc Clin N Am. 12:589–603. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Pennington L, Hamilton SR, Bayless TM and Cameron JL: Surgical management of Crohn's disease. Influence of disease at margin of resection. Ann Surg. 192:311–318. 1980. View Article : Google Scholar : PubMed/NCBI

7 

Han R, Rostami-Yazdi M, Gerdes S and Mrowietz U: Triptolide in the treatment of psoriasis and other immune-mediated inflammatory diseases. Br J Clin Pharmacol. 74:424–436. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Ren J, Tao Q, Wang X, Wang Z and Li J: Efficacy of T2 in active Crohn's disease: A prospective study report. Dig Dis Sci. 52:1790–1797. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Jiang C, Fang X, Zhang H, Wang X, Li M, Jiang W, Tian F, Zhu L and Bian Z: Triptolide inhibits the growth of osteosarcoma by regulating microRNA-181a via targeting PTEN gene in vivo and vitro. Tumour Biol. 39:10104283176975562017. View Article : Google Scholar : PubMed/NCBI

10 

Zhang Z, Qu X, Ni Y, Zhang K, Dong Z, Yan X, Qin J, Sun H, Ding Y, Zhao P and Gong K: Triptolide protects rat heart against pressure overload-induced cardiac fibrosis. Int J Cardiol. 168:2498–2505. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Hou HW, Wang JM, Wang D, Wu R and Ji ZL: Triptolide exerts protective effects against fibrosis following ileocolonic anastomosis by mechanisms involving the miR-16-1/HSP70 pathway in IL-10-deficient mice. Int J Mol Med. 40:337–346. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Li F, Xu Y, Deng S, Li Z, Zou D, Yi S, Sui W, Hao M and Qiu L: MicroRNA-15a/16-1 cluster located at chromosome 13q14 is down-regulated but displays different expression pattern and prognostic significance in multiple myeloma. Oncotarget. 6:38270–38282. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Bonci D, Coppola V, Musumeci M, Addario A, D'Urso L, Collura D, Peschle C, De Maria R and Muto G: The Mir-15a/Mir-16-1 cluster controls prostate cancer progression control by targeting of multiple oncogenic activities. J Urol. 181:1882009. View Article : Google Scholar

14 

Bottoni A, Piccin D, Tagliati F, Luchin A, Zatelli MC and degli Uberti EC: miR-15a and miR-16-1 down-regulation in pituitary adenomas. J Cell Physiol. 204:280–285. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Sam S, Sam MR, Esmaeillou M and Safaralizadeh R: Effective targeting survivin, caspase-3 and MicroRNA-16-1 expression by Methyl-3-pentyl-6-methoxyprodigiosene triggers apoptosis in colorectal cancer stem-like cells. Pathol Oncol Res. 24:715–723. 2016. View Article : Google Scholar

16 

Iborra M, Bernuzzi F, Correale C, Vetrano S, Fiorino G, Beltrán B, Marabita F, Locati M, Spinelli A, Nos P, et al: Identification of serum and tissue micro-RNA expression profiles in different stages of inflammatory bowel disease. Clinical and experimental immunology. 173:250–258. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Paraskevi A, Theodoropoulos G, Papaconstantinou I, Mantzaris G, Nikiteas N and Gazouli M: Circulating MicroRNA in inflammatory bowel disease. J Crohns Colitis. 6:900–904. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Sherman MY and Gabai VL: Hsp70 in cancer: Back to the future. Oncogene. 34:4153–4161. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Murphy ME: The HSP70 family and cancer. Carcinogenesis. 34:1181–1188. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Calderwood SK and Gong J: Heat shock proteins promote cancer: It's a protection racket. Trends Biochem Sci. 41:311–323. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Wang C, Zhang Y, Guo K, Wang N, Jin H, Liu Y and Qin W: Heat shock proteins in hepatocellular carcinoma: Molecular mechanism and therapeutic potential. Int J Cancer. 138:1824–1834. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Miller SB, Mogk A and Bukau B: Spatially organized aggregation of misfolded proteins as cellular stress defense strategy. J Mol Biol. 427:1564–1574. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Sevin M, Girodon F, Garrido C and de Thonel A: HSP90 and HSP70: Implication in inflammation processes and therapeutic approaches for myeloproliferative neoplasms. Mediators Inflamm. 2015:9702422015. View Article : Google Scholar : PubMed/NCBI

24 

Mohanan V and Grimes CL: The molecular chaperone HSP70 binds to and stabilizes NOD2, an important protein involved in Crohn disease. J Biol Chem. 289:18987–18998. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Bellaye PS, Burgy O, Causse S, Garrido C and Bonniaud P: Heat shock proteins in fibrosis and wound healing: Good or evil? Pharmacol Ther. 143:119–132. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Tanaka K, Tanaka Y, Namba T, Azuma A and Mizushima T: Heat shock protein 70 protects against bleomycin-induced pulmonary fibrosis in mice. Biochem Pharmacol. 80:920–931. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Zhang Z and Cheng Y: miR-16-1 promotes the aberrant alpha-synuclein accumulation in parkinson disease via targeting heat shock protein 70. ScientificWorldJournal. 2014:9383482014.PubMed/NCBI

28 

Wang S, Wang X, Yan J, Xie X, Fan F, Zhou X, Han L and Chen J: Resveratrol inhibits proliferation of cultured rat cardiac fibroblasts: Correlated with NO-cGMP signaling pathway. Eur J Pharmacol. 567:26–35. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Olson ER, Naugle JE, Zhang X, Bomser JA and Meszaros JG: Inhibition of cardiac fibroblast proliferation and myofibroblast differentiation by resveratrol. Am J Physiol Heart Circ Physiol. 288:H1131–H1138. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Shi H, Zhang X, He Z, Wu Z, Rao L and Li Y: Metabolites of hypoxic cardiomyocytes induce the migration of cardiac fibroblasts. Cell Physiol Biochem. 41:413–421. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Wang Y, Jia L and Wu CY: Triptolide inhibits the differentiation of Th17 cells and suppresses collagen-induced arthritis. Scand J Immunol. 68:383–390. 2010. View Article : Google Scholar

32 

Zhu KJ, Shen QY, Cheng H, Mao XH, Lao LM and Hao GL: Triptolide affects the differentiation, maturation and function of human dendritic cells. Int Immunopharmacol. 5:1415–1426. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Li Y, Yu C, Zhu WM, Xie Y, Qi X, Li N and Li JS: Triptolide ameliorates IL-10-deficient mice colitis by mechanisms involving suppression of IL-6/STAT3 signaling pathway and down-regulation of IL-17. Mol Immunol. 47:2467–2474. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Wei X, Gong J, Zhu J, Wang P, Li N, Zhu W and Li J: The suppressive effect of triptolide on chronic colitis and TNF-alpha/TNFR2 signal pathway in interleukin-10 deficient mice. Clin Immunol. 129:211–218. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Yu C, Shan T, Feng A, Li Y, Zhu W, Xie Y, Li N and Li J: Triptolide ameliorates Crohn's colitis is associated with inhibition of TLRs/NF-κB signaling pathway. Fitoterapia. 82:709–715. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Cao Y, Huang X, Fan Y and Chen X: Protective effect of triptolide against glomerular mesangial cell proliferation and glomerular fibrosis in rats involves the TGF-β 1/Smad signaling pathway. Evid Based Complement Alternat Med. 2015:8140892015. View Article : Google Scholar : PubMed/NCBI

38 

Chen C, Yang S, Zhang M, Zhang Z, Hong J, Han D, Ma J, Zhang SB, Okunieff P and Zhang L: Triptolide mitigates radiation-induced pulmonary fibrosis via inhibition of axis of alveolar macrophages-NOXes-ROS-myofibroblasts. Cancer Biol Ther. 17:381–389. 2016. View Article : Google Scholar : PubMed/NCBI

39 

Liu M, Chen J, Huang Y, Ke J, Li L, Huang D and Wu W: Triptolide alleviates isoprenaline-induced cardiac remodeling in rats via TGF-β1/Smad3 and p38 MAPK signaling pathway. Pharmazie. 70:244–250. 2015.PubMed/NCBI

40 

Wang F, Fu XD, Zhou Y and Zhang Y: Down-regulation of the cyclin E1 oncogene expression by microRNA-16-1 induces cell cycle arrest in human cancer cells. BMB Rep. 42:725–730. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Acunzo M and Croce CM: Downregulation of miR-15a and miR-16-1 at 13q14 in chronic lymphocytic leukemia. Clin Chem. 62:655–656. 2016. View Article : Google Scholar : PubMed/NCBI

42 

Cai CK, Zhao GY, Tian LY, Liu L, Yan K, Ma YL, Ji ZW, Li XX, Han K, Gao J, et al: miR-15a and miR-16-1 downregulate CCND1 and induce apoptosis and cell cycle arrest in osteosarcoma. Oncol Rep. 28:1764–1770. 2012. View Article : Google Scholar : PubMed/NCBI

43 

Meng HT, Zhu L, Ni WM, You LS, Jin J and Qian WB: Triptolide inhibits the proliferation of cells from lymphocytic leukemic cell lines in association with downregulation of NF-κB activity and miR-16-1*. Acta Pharmacol Sin. 32:503–511. 2011. View Article : Google Scholar : PubMed/NCBI

44 

Klausz G, Molnár T, Nagy F, Gyulai Z, Boda K, Lonovics J and Mándi Y: Polymorphism of the heat-shock protein gene Hsp70-2, but not polymorphisms of the IL-10 and CD14 genes, is associated with the outcome of Crohn's disease. Scand J Gastroenterol. 40:1197–1204. 2009. View Article : Google Scholar

45 

Li Y, Kang X and Wang Q: HSP70 decreases receptor-dependent phosphorylation of Smad2 and blocks TGF-β-induced epithelial-mesenchymal transition. J Genet Genomics. 38:111–116. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Yun CH, Yoon SY, Nguyen TT, Cho HY, Kim TH, Kim ST, Kim BC, Hong YS, Kim SJ and Lee HJ: Geldanamycin inhibits TGF-beta signaling through induction of Hsp70. Arch Biochem Biophys. 495:8–13. 2010. View Article : Google Scholar : PubMed/NCBI

47 

Fujibayashi T, Hashimoto N, Jijiwa M, Hasegawa Y, Kojima T and Ishiguro N: Protective effect of geranylgeranylacetone, an inducer of heat shock protein 70, against drug-induced lung injury/fibrosis in an animal model. BMC Pulm Med. 9:452009. View Article : Google Scholar : PubMed/NCBI

48 

He W, Zhuang Y, Wang L, Qi L, Chen B, Wang M, Shao D and Chen J: Geranylgeranylacetone attenuates hepatic fibrosis by increasing the expression of heat shock protein 70. Mol Med Rep. 12:4895–4900. 2015. View Article : Google Scholar : PubMed/NCBI

49 

Mao H, Li Z, Zhou Y, Li Z, Zhuang S, An X, Zhang B, Chen W, Nie J, Wang Z, et al: HSP72 attenuates renal tubular cell apoptosis and interstitial fibrosis in obstructive nephropathy. Am J Physiol Renal Physiol. 295:F202–F214. 2008. View Article : Google Scholar : PubMed/NCBI

50 

Zhou Y, Mao H, Li S, Cao S, Li Z, Zhuang S, Fan J, Dong X, Borkan SC, Wang Y and Yu X: HSP72 inhibits Smad3 activation and nuclear translocation in renal epithelial-to-mesenchymal transition. J Am Soc Nephrol. 21:598–609. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen M, Wang JM, Wang D, Wu R and Hou HW: Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549. Mol Med Rep 19: 4841-4851, 2019.
APA
Chen, M., Wang, J., Wang, D., Wu, R., & Hou, H. (2019). Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549. Molecular Medicine Reports, 19, 4841-4851. https://doi.org/10.3892/mmr.2019.10117
MLA
Chen, M., Wang, J., Wang, D., Wu, R., Hou, H."Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549". Molecular Medicine Reports 19.6 (2019): 4841-4851.
Chicago
Chen, M., Wang, J., Wang, D., Wu, R., Hou, H."Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549". Molecular Medicine Reports 19, no. 6 (2019): 4841-4851. https://doi.org/10.3892/mmr.2019.10117
Copy and paste a formatted citation
x
Spandidos Publications style
Chen M, Wang JM, Wang D, Wu R and Hou HW: Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549. Mol Med Rep 19: 4841-4851, 2019.
APA
Chen, M., Wang, J., Wang, D., Wu, R., & Hou, H. (2019). Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549. Molecular Medicine Reports, 19, 4841-4851. https://doi.org/10.3892/mmr.2019.10117
MLA
Chen, M., Wang, J., Wang, D., Wu, R., Hou, H."Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549". Molecular Medicine Reports 19.6 (2019): 4841-4851.
Chicago
Chen, M., Wang, J., Wang, D., Wu, R., Hou, H."Triptolide inhibits migration and proliferation of fibroblasts from ileocolonic anastomosis of patients with Crohn's disease via regulating the miR‑16‑1/HSP70 pathway Retraction in /10.3892/mmr.2025.13549". Molecular Medicine Reports 19, no. 6 (2019): 4841-4851. https://doi.org/10.3892/mmr.2019.10117
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