Zinc finger E‑box binding homeobox 2 functions as an oncogene 
in human laryngeal squamous cell carcinoma

Li,Qun; Ma,Liang; Wu,Zhenhua; Wang,Guoli; Huang,Qi; Shen,Zhisen; Yu,Rui

doi:10.3892/mmr.2019.10126

Zinc finger E‑box binding homeobox 2 functions as an oncogene in human laryngeal squamous cell carcinoma

Authors:
- Qun Li
- Liang Ma
- Zhenhua Wu
- Guoli Wang
- Qi Huang
- Zhisen Shen
- Rui Yu
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Affiliations: Department of Otorhinolaryngology, Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, Zhejiang 315040, P.R. China, Department of Biochemistry, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center, Lihuili Eastern Hospital, Ningbo, Zhejiang 315030, P.R. China
Published online on: April 4, 2019 https://doi.org/10.3892/mmr.2019.10126
Pages: 4545-4552
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Zinc finger E‑box binding homeobox 2 (ZEB2) is a member of the Zfh1 family of two‑handed zinc finger/homeodomain proteins. To date, the role of ZEB2 in human laryngeal carcinoma has not been clearly defined. In the present study, the level of ZEB2 expression in laryngeal squamous cell carcinoma (LSCC) tissues and adjacent normal tissues was evaluated using reverse transcription‑quantitative polymerase chain reaction. The effects of ZEB2 on the growth, migration, invasion, cell cycle distribution and apoptosis of laryngeal cancer cells were also explored using MTT, Transwell and flow cytometry assays. It was identified that ZEB2 was upregulated in LSCC tissues compared with normal tissues. Silencing of ZEB2 inhibited the viability, migration and invasion of LSCC cells. It was also observed that ZEB2 silencing induced cell cycle arrest and apoptosis in LSCC cells. Furthermore, ZEB2 silencing inhibited the process of epithelial‑mesenchymal transition. Overall, the results indicated that ZEB2 promotes the progression of LSCC and that it may be a potential target for the treatment of this type of cancer.

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