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Article

Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53

  • Authors:
    • Xueyan Fu
    • Yawei Wang
    • Gang Wu
    • Wanchuan Zhang
    • Shaolin Xu
    • Wenqing Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Geriatric Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 4998-5006
    |
    Published online on: April 12, 2019
       https://doi.org/10.3892/mmr.2019.10159
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Abstract

Emerging evidence suggests that long noncoding RNAs (lncRNAs) serve a key role in malignant transformation, tumor progression and metastasis. Increased expression of lncRNA p53 upregulated regulator of P53 levels (PURPL) has been reported to promote tumorigenicity in colorectal cancer; however, the role and potential mechanisms of PURPL in the development of liver cancer remain unclear. We employed reverse transcription‑quantitative polymerase chain reaction to detect PURPL and p53 mRNA expression in liver cancer tissues and cell lines. Cell Counting Kit‑8 and colony‑forming assays were used to examine the cell proliferation; whereas, flow cytometry was applied to detect apoptosis and cell cycle progression. p53 expression was detected by western blotting. The results revealed that PURPL was significantly upregulated in liver cancer tissues compared with in paracancerous tissues, and was associated with tumor differentiation stage and tumor size. PURPL was also upregulated in various liver cancer cell lines. Silencing of PURPL inhibited liver cancer cells proliferation, blocked cell cycle progression, and promoted apoptosis. Most importantly, PURPL expression was negatively correlated with p53 mRNA expression. In summary, lncRNA‑PURPL was proposed to promote cell proliferation in liver cancer by regulating the p53 gene. As such, it could serve as a potential therapeutic target for the diagnosis and treatment of liver cancer.
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1 

Shi X, Zhu HR, Liu TT, Shen XZ and Zhu JM: The Hippo pathway in hepatocellular carcinoma: Non-coding RNAs in action. Cancer Lett. 400:175–182. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Fujiwara N, Friedman SL, Goossens N and Hoshida Y: Risk factors and prevention of hepatocellular carcinoma in the era of precision medicine. J Hepatol. 68:526–549. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Ponting CP, Oliver PL and Reik W: Evolution and functions of long noncoding RNAs. Cell. 136:629–641. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Ma M, Cai B, Jiang L, Abdalla BA, Li Z, Nie Q and Zhang X: lncRNA-Six1 Is a target of miR-1611 that functions as a ceRNA to regulate six1 protein expression and fiber type switching in chicken myogenesis. Cells. 7(pii): E2432018. View Article : Google Scholar : PubMed/NCBI

6 

Grelet S, Link LA, Howley B, Obellianne C, Palanisamy V, Gangaraju VK, Diehl JA and Howe PH: A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. Nat Cell Biol. 19:1105–1115. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Ferrè F, Colantoni A and Helmer-Citterich M: Revealing protein-lncRNA interaction. Brief Bioinform. 17:106–116. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Zhu Y, Bian Y, Zhang Q, Hu J, Li L, Yang M, Qian H, Yu L, Liu B and Qian X: Construction and analysis of dysregulated lncRNA-associated ceRNA network in colorectal cancer. J Cell Biochem. Dec 7–2018.(Epub ahead of print).

9 

Jiang N, Wang X, Xie X, Liao Y, Liu N, Liu J, Miao N, Shen J and Peng T: lncRNA DANCR promotes tumor progression and cancer stemness features in osteosarcoma by upregulating AXL via miR-33a-5p inhibition. Cancer Lett. 405:46–55. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Li W, Jia G, Qu Y, Du Q and Liu B and Liu B: Long non-coding RNA (LncRNA) HOXA11-AS promotes breast cancer invasion and metastasis by regulating epithelial-mesenchymal transition. Med Sci Monit. 23:3393–3403. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Hu B, Cai H, Zheng R, Yang S, Zhou Z and Tu J: Long non-coding RNA 657 suppresses hepatocellular carcinoma cell growth by acting as a molecular sponge of miR-106a-5p to regulate PTEN expression. Int J Biochem Cell Biol. 92:34–42. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Mineo M, Ricklefs F, Rooj AK, Lyons SM, Ivanov P, Ansari KI, Nakano I, Chiocca EA, Godlewski J and Bronisz A: The long non-coding RNA HIF1A-AS2 facilitates the maintenance of mesenchymal glioblastoma stem-like cells in hypoxic niches. Cell Rep. 15:2500–2509. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Zhang HY, Zheng FS, Yang W and Lu JB: The long non-coding RNA MIAT regulates zinc finger E-box binding homeobox 1 expression by sponging miR-150 and promoteing cell invasion in non-small-cell lung cancer. Gene. 633:61–65. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Li XL, Subramanian M, Jones MF, Chaudhary R, Singh DK, Zong X, Gryder B, Sindri S, Mo M, Schetter A, et al: Long noncoding RNA PURPL suppresses basal p53 levels and promotes tumorigenicity in colorectal cancer. Cell Rep. 20:2408–2423. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Zhang T, Liu W, Meng W, Zhao H, Yang Q, Gu SJ, Xiao CC, Jia CC and Fu BS: Downregulation of miR-542-3p promotes cancer metastasis through activating TGF-β/Smad signaling in hepatocellular carcinoma. Onco Targets Ther. 11:1929–1939. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Ma Y, Yang Y, Wang F, Moyer MP, Wei Q, Zhang P, Yang Z, Liu W, Zhang H, Chen N, et al: Long non-coding RNA CCAL regulates colorectal cancer progression by activating Wnt/β-catenin signalling pathway via suppression of activator protein 2α. Gut. 65:1494–1504. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Wu G, Zheng K, Xia S, Wang Y, Meng X, Qin X and Cheng Y: MicroRNA-655-3p functions as a tumor suppressor by regulating ADAM10 and β-catenin pathway in Hepatocellular Carcinoma. J Exp Clin Cancer Res. 35:892016. View Article : Google Scholar : PubMed/NCBI

19 

Lohitesh K, Chowdhury R and Mukherjee S: Resistance a major hindrance to chemotherapy in hepatocellular carcinoma: An insight. Cancer Cell Int. 18:442018. View Article : Google Scholar : PubMed/NCBI

20 

Wang Y, Chen F, Zhao M, Yang Z, Li J, Zhang S, Zhang W, Ye L and Zhang X: The long noncoding RNA HULC promotes liver cancer by increasing the expression of the HMGA2 oncogene via sequestration of the microRNA-186. J Biol Chem. 292:15395–15407. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Zhang J: Long noncoding RNA TSLNC8 is a tumor suppressor that inactivates the interleukin-6/STAT3 signaling pathway. Hepatology. 67:171–187. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Liu Y, Yang Y, Wang T, Wang L, Wang X, Li T, Shi Y and Wang Y: Long non-coding RNA CCAL promotes hepatocellular carcinoma progression by regulating AP-2α and Wnt/β-catenin pathway. Int J Biol Macromol. 109:424–434. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Vogelstein B, Lane D and Levine AJ: Surfing the p53 network. Nature. 408:307–310. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Vousden KH and Lu X: Live or let die: The cell's response to p53. Nat Rev Cancer. 2:594–604. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Zhu H, Wang J, Yin J, Lu B, Yang Q, Wan Y and Jia C: Downregulation of PRAME suppresses proliferation and promotes apoptosis in hepatocellular carcinoma through the activation of P53 mediated pathway. Cell Physiol Biochem. 45:1121–1135. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Meng X, Franklin DA, Dong J and Zhang Y: MDM2-p53 pathway in hepatocellular carcinoma. Cancer Res. 74:7161–7167. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Su P, Wang F, Qi B, Wang T and Zhang S: P53 Regulation-association long non-coding RNA (LncRNA PRAL) inhibits cell proliferation by regulation of P53 in human lung cancer. Med Sci Monit. 23:1751–1758. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Pollutri D, Gramantieri L, Bolondi L and Fornari F: TP53/MicroRNA interplay in hepatocellular carcinoma. Int J Mol Sci. 17(pii): E20292016. View Article : Google Scholar : PubMed/NCBI

29 

Liu J, Rao J, Lou X, Zhai J, Ni Z and Wang X: Upregulated TRIM11 exerts its oncogenic effects in hepatocellular carcinoma through inhibition of P53. Cell Physiol Biochem. 44:255–266. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Zhou CC, Yang F, Yuan SX, Ma JZ, Liu F, Yuan JH, Bi FR, Lin KY, Yin JH, Cao GW, et al: Systemic genome screening identifies the outcome associated focal loss of long noncoding RNA PRAL in hepatocellular carcinoma. Hepatology. 63:850–863. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Ren Y, Shang J, Li J, Liu W, Zhang Z, Yuan J and Yang M: The long noncoding RNA PCAT-1 links the microRNA miR-215 to oncogene CRKL-mediated signaling in hepatocellular carcinoma. J Biol Chem. 292:17939–17949. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Zhang M, Wang W, Li T, Yu X, Zhu Y, Ding F, Li D and Yang T: Long noncoding RNA SNHG1 predicts a poor prognosis and promotes hepatocellular carcinoma tumorigenesis. Biomed Pharmacother. 80:73–79. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fu X, Wang Y, Wu G, Zhang W, Xu S and Wang W: Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53. Mol Med Rep 19: 4998-5006, 2019.
APA
Fu, X., Wang, Y., Wu, G., Zhang, W., Xu, S., & Wang, W. (2019). Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53. Molecular Medicine Reports, 19, 4998-5006. https://doi.org/10.3892/mmr.2019.10159
MLA
Fu, X., Wang, Y., Wu, G., Zhang, W., Xu, S., Wang, W."Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53". Molecular Medicine Reports 19.6 (2019): 4998-5006.
Chicago
Fu, X., Wang, Y., Wu, G., Zhang, W., Xu, S., Wang, W."Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53". Molecular Medicine Reports 19, no. 6 (2019): 4998-5006. https://doi.org/10.3892/mmr.2019.10159
Copy and paste a formatted citation
x
Spandidos Publications style
Fu X, Wang Y, Wu G, Zhang W, Xu S and Wang W: Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53. Mol Med Rep 19: 4998-5006, 2019.
APA
Fu, X., Wang, Y., Wu, G., Zhang, W., Xu, S., & Wang, W. (2019). Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53. Molecular Medicine Reports, 19, 4998-5006. https://doi.org/10.3892/mmr.2019.10159
MLA
Fu, X., Wang, Y., Wu, G., Zhang, W., Xu, S., Wang, W."Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53". Molecular Medicine Reports 19.6 (2019): 4998-5006.
Chicago
Fu, X., Wang, Y., Wu, G., Zhang, W., Xu, S., Wang, W."Long noncoding RNA PURPL promotes cell proliferation in liver cancer by regulating p53". Molecular Medicine Reports 19, no. 6 (2019): 4998-5006. https://doi.org/10.3892/mmr.2019.10159
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