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Article Open Access

miR‑148 family members are putative biomarkers for sepsis

  • Authors:
    • Lei Dong
    • Hongwei Li
    • Shunli Zhang
    • Guanzheng Yang
  • View Affiliations / Copyright

    Affiliations: Intensive Care Unit, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Pediatrics, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Respiratory Medicine, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5133-5141
    |
    Published online on: April 19, 2019
       https://doi.org/10.3892/mmr.2019.10174
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Abstract

Sepsis is a type of systemic inflammatory response caused by infection. The present study aimed to identify novel targets for the treatment of sepsis. We conducted bioinformatic analysis of the microarray Gene Expression Omnibus dataset GSE12624, which includes data on 34 patients with sepsis and 36 healthy individuals without sepsis. Differentially expressed genes (DEGs) in sepsis patients were identified using Bayesian methods included in the limma package in R. Correlations among the expression values of DEGs were analyzed using the weighted gene co‑expression network analysis (WGCNA) to construct a co‑expression network. Subsequently, the generated co‑expression network was visualized using Cytoscape 3.3 software. Additionally, a protein‑protein interaction (PPI) network was constructed based on all the DEGs using STRING. Finally, the integrated regulatory network was constructed based on DEGs, microRNAs (miRNAs) and transcription factors (TFs). A total of 407 DEGs were identified in the sepsis samples, including 227 upregulated DEGs and 180 downregulated DEGs. WGCNA grouped the DEGs into 13 co‑expressed modules. Additionally, MAP3K8 and RPS6KA5 in the MEyellow module were enriched in the MAPK and TNF signaling pathways. In addition, the PPI network comprised 48 nodes and 112 edges, which included the pairs MAP3K8‑RPS6KA5, MAP3K8‑IL10, RPS6KA5‑EXOSC4 and EXOSC4‑EXOSC5. Lastly, the TF‑miRNA‑target DEG regulatory network was constructed based on eight TFs (NF‑κB), seven miRNAs (miR152, miR‑148A/B), and 52 TF‑miRNA‑target gene triplets (17 upregulated genes, including MAP3K8, and 10 downregulated genes, including RPS6KA5). Our analysis showed that the members of the miR‑148 family (miR‑148A/B and miR‑152) are candidate biomarkers for sepsis.
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1 

Rhodes A, Evans LE, Alhazzani W, Levy MM, Antonelli M, Ferrer R, Kumar A, Sevransky JE, Sprung CL, Nunnally ME, et al: Surviving sepsis campaign: International guidelines for management of sepsis and septic shock: 2016. Crit Care Med. 45:486–552. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Tsai D, Stewart P, Goud R, Gourley S, Hewagama S, Krishnaswamy S, Wallis SC, Lipman J and Roberts JA: Total and unbound ceftriaxone pharmacokinetics in critically ill Australian Indigenous patients with severe sepsis. Int J Antimicrob Agents. 48:748–752. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Fleischmann C, Scherag A, Adhikari NK, Hartog CS, Tsaganos T, Schlattmann P, Angus DC and Reinhart K; International Forum of Acute Care Trialists, : Assessment of global incidence and mortality of hospital-treated sepsis.current estimates and limitations. Am J Respir Crit Care Med. 193:259–272. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Cheng W, Wang S, Shen C, Zhao D, Li D and Shang Y: Epidemiology of hospitalized burns patients in china: A Systematic Review. Burn Open. 2:8–16. 2017. View Article : Google Scholar

5 

Mitra P, Guha D, Nag SS, Mondal BC and Dasgupta S: Role of plasma fibrinogen in diagnosis and prediction of short term outcome in neonatal sepsis. Indian J Hematol Blood Transfus. 33:195–199. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Kang S, Tanaka T, Masuda K and Kishimoto T: Implications of IL-6 Targeting Therapy for Sepsis. Immunotherapy (Los Angel). 3:1382017. View Article : Google Scholar

7 

Kurt AN, Aygun AD, Godekmerdan A, Kurt A, Dogan Y and Yilmaz E: Serum IL-1beta, IL-6, IL-8, and TNF-alpha levels in early diagnosis and management of neonatal sepsis. Mediators Inflamm. 2007:313972007. View Article : Google Scholar : PubMed/NCBI

8 

Thimmulappa RK, Lee H, Rangasamy T, Reddy SP, Yamamoto M, Kensler TW and Biswal S: Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis. J Clin Invest. 116:984–995. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Schuetz P, Birkhahn R, Sherwin R, Jones AE, Singer A, Kline JA, Runyon MS, Self WH, Courtney DM, Nowak RM, et al: Serial procalcitonin predicts mortality in severe sepsis patients: Results from the multicenter procalcitonin monitoring sepsis (MOSES) Study. Crit Care Med. 45:781–789. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Riedel S: Procalcitonin and the role of biomarkers in the diagnosis and management of sepsis. Diagn Microbiol Infect Dis. 73:221–227. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Wacker C, Prkno A, Brunkhorst FM and Schlattmann P: Procalcitonin as a diagnostic marker for sepsis: A systematic review and meta-analysis. Lancet Infect Dis. 13:426–435. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Wu X, Yang J, Yu L and Long D: Plasma miRNA-223 correlates with risk, inflammatory markers as well as prognosis in sepsis patients. Medicine (Baltimore). 97:e113522018. View Article : Google Scholar : PubMed/NCBI

13 

Bao YYX and Chen Z: The early diagnostic value of microRNA-223 for patients with complication of sepsis after ureteroscopic lithotrity. Chin J Integr Tradit West Med Intensive Crit Care. 24:465–468. 2017.(In Chinese).

14 

Tili E, Michaille JJ, Cimino A, Costinean S, Dumitru CD, Adair B, Fabbri M, Alder H, Liu CG, Calin GA and Croce CM: Modulation of miR-155 and miR-125b levels following lipopolysaccharide/TNF-alpha stimulation and their possible roles in regulating the response to endotoxin shock. J Immunol. 179:5082–5089. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Menges T, König IR, Hossain H, Little S, Tchatalbachev S, Thierer F, Hackstein H, Franjkovic I, Colaris T, Martens F, et al: Sepsis syndrome and death in trauma patients are associated with variation in the gene encoding tumor necrosis factor. Crit Care Med. 36:1456–1462, e1-e6. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Langfelder P and Horvath S: WGCNA: An R package for weighted correlation network analysis. BMC Bioinformatics. 9:5592008. View Article : Google Scholar : PubMed/NCBI

17 

Huang W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J, Simonovic M, Roth A, Santos A, Tsafou KP, et al: STRING v10: Protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res. 43(D1): D447–D452. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Bandettini WP, Kellman P, Mancini C, Booker OJ, Vasu S, Leung SW, Wilson JR, Shanbhag SM, Chen MY and Arai AE: MultiContrast Delayed Enhancement (MCODE) improves detection of subendocardial myocardial infarction by late gadolinium enhancement cardiovascular magnetic resonance: A clinical validation study. J Cardiovasc Magn Reson. 14:832012. View Article : Google Scholar : PubMed/NCBI

21 

Mielke LA, Elkins KL, Wei L, Starr R, Tsichlis PN, O'Shea JJ and Watford WT: Tumor progression locus 2 (Map3k8) is critical for host defense against Listeria monocytogenes and IL-1 beta production. J Immunol. 183:7984–7993. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Leon LR, White AA and Kluger MJ: Role of IL-6 and TNF in thermoregulation and survival during sepsis in mice. Am J Physiol. 275:R269–R277. 1998.PubMed/NCBI

23 

Stuber F, Udalova IA, Book M, Drutskaya LN, Kuprash DV, Turetskaya RL, Schade FU and Nedospasov SA: −308 tumor necrosis factor (TNF) polymorphism is not associated with survival in severe sepsis and is unrelated to lipopolysaccharide inducibility of the human TNF promoter. J Inflamm. 46:42–50. 1995-1996.

24 

Riedemann NC, Guo RF and Ward PA: Novel strategies for the treatment of sepsis. Nat Med. 9:517–524. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Oberholzer C, Oberholzer A, Bahjat FR, Minter RM, Tannahill CL, Abouhamze A, LaFace D, Hutchins B, Clare-Salzler MJ and Moldawer LL: Targeted adenovirus-induced expression of IL-10 decreases thymic apoptosis and improves survival in murine sepsis. Proc Natl Acad Sci USA. 98:11503–11508. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Filgueiras LR Jr, Martins JO, Serezani CH, Capelozzi VL, Montes MBA and Jancar S: Sepsis-induced acute lung injury (ALI) is milder in diabetic rats and correlates with impaired NFkB activation. PLoS One. 7:e449872012. View Article : Google Scholar : PubMed/NCBI

27 

Li Q and Verma IM: NF-kappaB regulation in the immune system. Nat Rev Immunol. 2:725–734. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Karin M: Nuclear factor-kappaB in cancer development and progression. Nature. 441:431–436. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Jiang C, Yu L, Tu Q, Zhao Y, Zhang H and Zhao S: Assignment of a member of the ribosomal protein S6 kinase family, RPS6KA5, to human chromosome 14q31-->q32.1 by radiation hybrid mapping. Cytogenet Cell Genet. 87:261–262. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Funding AT, Johansen C, Kragballe K, Otkjaer K, Jensen UB, Madsen MW, Fjording MS, Finnemann J, Skak-Nielsen T, Paludan SR and Iversen L: Mitogen- and stress-activated protein kinase 1 is activated in lesional psoriatic epidermis and regulates the expression of pro-inflammatory cytokines. J Invest Dermatol. 126:1784–1791. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Liu X, Zhan Z, Xu L, Ma F, Li D, Guo Z, Li N and Cao X: MicroRNA-148/152 impair innate response and antigen presentation of TLR-triggered dendritic cells by targeting CaMKIIα. J Immunol. 185:7244–7251. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Aslam R, Speck ER, Kim M, Crow AR, Bang KW, Nestel FP, Ni H, Lazarus AH, Freedman J and Semple JW: Platelet Toll-like receptor expression modulates lipopolysaccharide-induced thrombocytopenia and tumor necrosis factor-alpha production in vivo. Blood. 107:637–641. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Eidson LN, Inoue K, Young LJ, Tansey MG and Murphy AZ: Toll-like receptor 4 mediates morphine-induced neuroinflammation and tolerance via soluble tumor necrosis factor signaling. Neuropsychopharmacology. 42:661–670. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Holst B, Szakmany T, Raby AC, Hamlyn V, Durno K, Hall JE and Labéta MO: Soluble Toll-like receptor 2 is a biomarker for sepsis in critically ill patients with multi-organ failure within 12 h of ICU admission. Intensive Care Med Exp. 5:22017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Dong L, Li H, Zhang S and Yang G: miR‑148 family members are putative biomarkers for sepsis. Mol Med Rep 19: 5133-5141, 2019.
APA
Dong, L., Li, H., Zhang, S., & Yang, G. (2019). miR‑148 family members are putative biomarkers for sepsis. Molecular Medicine Reports, 19, 5133-5141. https://doi.org/10.3892/mmr.2019.10174
MLA
Dong, L., Li, H., Zhang, S., Yang, G."miR‑148 family members are putative biomarkers for sepsis". Molecular Medicine Reports 19.6 (2019): 5133-5141.
Chicago
Dong, L., Li, H., Zhang, S., Yang, G."miR‑148 family members are putative biomarkers for sepsis". Molecular Medicine Reports 19, no. 6 (2019): 5133-5141. https://doi.org/10.3892/mmr.2019.10174
Copy and paste a formatted citation
x
Spandidos Publications style
Dong L, Li H, Zhang S and Yang G: miR‑148 family members are putative biomarkers for sepsis. Mol Med Rep 19: 5133-5141, 2019.
APA
Dong, L., Li, H., Zhang, S., & Yang, G. (2019). miR‑148 family members are putative biomarkers for sepsis. Molecular Medicine Reports, 19, 5133-5141. https://doi.org/10.3892/mmr.2019.10174
MLA
Dong, L., Li, H., Zhang, S., Yang, G."miR‑148 family members are putative biomarkers for sepsis". Molecular Medicine Reports 19.6 (2019): 5133-5141.
Chicago
Dong, L., Li, H., Zhang, S., Yang, G."miR‑148 family members are putative biomarkers for sepsis". Molecular Medicine Reports 19, no. 6 (2019): 5133-5141. https://doi.org/10.3892/mmr.2019.10174
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