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Article

Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients

  • Authors:
    • Noriaki Nagai
    • Yu Mano
    • Hiroko Otake
    • Teppei Shibata
    • Eri Kubo
    • Hiroshi Sasaki
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Kindai University, Higashi‑Osaka, Osaka 577‑8502, Japan, Department of Ophthalmology, Kanazawa Medical University, Kanazawa, Ishikawa 920‑0293, Japan
  • Pages: 5464-5472
    |
    Published online on: May 2, 2019
       https://doi.org/10.3892/mmr.2019.10214
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Abstract

We previously reported that the collapse of ATP production via mitochondrial damage causes ATPase dysfunction, resulting in the onset or progression of lens opacification in cataracts in model rats. In the present study, it was investigated whether the mRNA expression levels of the three subtypes of mitochondrial cytochrome c oxidase (MTCO)1, 2 and 3 and ATP content change with the type and severity of cataracts in human lens. Samples of lens epithelium were collected from Japanese patients during cataract surgery, and the type and severity of the cataracts (grade) were determined according to the WHO classification [cortical (COR), nuclear (NUC), posterior subcapsular (PSC) opacification]. The MTCO1‑3 mRNA expression levels in patients with grade‑1 COR, NUC and PSC opacification were significantly enhanced compared with those of normal patients. The enhanced MTCO1‑3 mRNA levels subsequently decreased in patients with COR, and the MTCO1‑3 mRNA levels and ATP levels in patients with grade‑3 COR were similar to those in normal patients. However, the mRNA expression levels of MTCO3 in patients with grade 3‑NUC opacification and MTCO1‑3 in patients with grade‑3 PSC opacification, along with the ATP content, were significantly lower than in patients without cataracts. In conclusion, it was revealed that ATP production in lens epithelium is enhanced in early‑stage cataracts (grade‑1) in Japanese patients with COR, NUC and PSC opacification. In addition, in severe cataracts (grade‑3), ATP production and content are strongly decreased in Japanese patients with PSC opacification. ATP depletion in human lens epithelium with PSC opacification may promote lens opacification by ATPase dysfunction.
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Copy and paste a formatted citation
Spandidos Publications style
Nagai N, Mano Y, Otake H, Shibata T, Kubo E and Sasaki H: Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients. Mol Med Rep 19: 5464-5472, 2019.
APA
Nagai, N., Mano, Y., Otake, H., Shibata, T., Kubo, E., & Sasaki, H. (2019). Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients. Molecular Medicine Reports, 19, 5464-5472. https://doi.org/10.3892/mmr.2019.10214
MLA
Nagai, N., Mano, Y., Otake, H., Shibata, T., Kubo, E., Sasaki, H."Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients". Molecular Medicine Reports 19.6 (2019): 5464-5472.
Chicago
Nagai, N., Mano, Y., Otake, H., Shibata, T., Kubo, E., Sasaki, H."Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients". Molecular Medicine Reports 19, no. 6 (2019): 5464-5472. https://doi.org/10.3892/mmr.2019.10214
Copy and paste a formatted citation
x
Spandidos Publications style
Nagai N, Mano Y, Otake H, Shibata T, Kubo E and Sasaki H: Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients. Mol Med Rep 19: 5464-5472, 2019.
APA
Nagai, N., Mano, Y., Otake, H., Shibata, T., Kubo, E., & Sasaki, H. (2019). Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients. Molecular Medicine Reports, 19, 5464-5472. https://doi.org/10.3892/mmr.2019.10214
MLA
Nagai, N., Mano, Y., Otake, H., Shibata, T., Kubo, E., Sasaki, H."Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients". Molecular Medicine Reports 19.6 (2019): 5464-5472.
Chicago
Nagai, N., Mano, Y., Otake, H., Shibata, T., Kubo, E., Sasaki, H."Changes in mitochondrial cytochrome c oxidase mRNA levels with cataract severity in lens epithelia of Japanese patients". Molecular Medicine Reports 19, no. 6 (2019): 5464-5472. https://doi.org/10.3892/mmr.2019.10214
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