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miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways

  • Authors:
    • Jia Liu
    • Linggai Cao
    • Na Zhao
    • Yue Feng
    • Ze Yu
    • Yuhua Li
    • Chunbo Teng
    • Jifan Hu
    • Tao Li
  • View Affiliations / Copyright

    Affiliations: Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China, Department of Basic Science, Stanford University Medical School, Palo Alto Veterans Institute for Research, Palo Alto, CA 94305, USA
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 33-40
    |
    Published online on: May 9, 2019
       https://doi.org/10.3892/mmr.2019.10215
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Abstract

The aim of the present study was to explore the underlying mechanism of microRNA‑338‑3p (miR‑338‑3p) in lung cancer cell (A549) invasion and proliferation. A microarray assay showed that miR‑338‑3p upregulated 216 and downregulated 147 genes in A549 cells, and the differentially expressed genes were enriched for several signaling pathways, including AKT and β‑catenin signaling pathways. Western blotting results showed that phosphorylated (p)‑AKT at Ser473 and at Thr308 and p‑β‑catenin at Ser552 were downregulated. Inhibiting AKT signaling pathway decreased β‑catenin signaling pathway. Overexpression of β‑catenin promoted the invasion of A549 cells. In addition, miR‑338‑3p showed inhibitory effect on the primary tumor developed from KrasG12D mice. Together, the data of the present study support that miR‑338‑3p inhibits lung cancer cell invasion and proliferation by downregulating AKT/β‑catenin signaling pathway. The present findings supported the potential use of miR‑338‑3p in the treatment of lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Cao L, Zhao N, Feng Y, Yu Z, Li Y, Teng C, Hu J and Li T: miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways. Mol Med Rep 20: 33-40, 2019.
APA
Liu, J., Cao, L., Zhao, N., Feng, Y., Yu, Z., Li, Y. ... Li, T. (2019). miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways. Molecular Medicine Reports, 20, 33-40. https://doi.org/10.3892/mmr.2019.10215
MLA
Liu, J., Cao, L., Zhao, N., Feng, Y., Yu, Z., Li, Y., Teng, C., Hu, J., Li, T."miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways". Molecular Medicine Reports 20.1 (2019): 33-40.
Chicago
Liu, J., Cao, L., Zhao, N., Feng, Y., Yu, Z., Li, Y., Teng, C., Hu, J., Li, T."miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways". Molecular Medicine Reports 20, no. 1 (2019): 33-40. https://doi.org/10.3892/mmr.2019.10215
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Cao L, Zhao N, Feng Y, Yu Z, Li Y, Teng C, Hu J and Li T: miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways. Mol Med Rep 20: 33-40, 2019.
APA
Liu, J., Cao, L., Zhao, N., Feng, Y., Yu, Z., Li, Y. ... Li, T. (2019). miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways. Molecular Medicine Reports, 20, 33-40. https://doi.org/10.3892/mmr.2019.10215
MLA
Liu, J., Cao, L., Zhao, N., Feng, Y., Yu, Z., Li, Y., Teng, C., Hu, J., Li, T."miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways". Molecular Medicine Reports 20.1 (2019): 33-40.
Chicago
Liu, J., Cao, L., Zhao, N., Feng, Y., Yu, Z., Li, Y., Teng, C., Hu, J., Li, T."miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways". Molecular Medicine Reports 20, no. 1 (2019): 33-40. https://doi.org/10.3892/mmr.2019.10215
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