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Article Open Access

Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury

  • Authors:
    • Xi‑Meng Yi
    • Yi Chen
    • Guan‑Jun Tu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Yi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 41-48
    |
    Published online on: May 9, 2019
       https://doi.org/10.3892/mmr.2019.10217
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Abstract

The present study was designed to investigate the effect of neuregulin‑1 (NRG1) on the migration of rat bone marrow mesenchymal stem cells (BMSCs) and evaluate the role of NRG1 in the functional recovery following spinal cord injury (SCI). Firstly, the effect of NRG1 on the mRNA expression of Snail in the BMSCs was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis; secondly, the BMSCs were transfected with a Snail‑overexpression plasmid (pBabe‑puro‑Snail) and the expression levels of Snail and matrix metalloptoreinase‑2 (MMP‑2) were detected by RT‑qPCR and western blot analyses; thirdly, the cell proliferation and migration of BMSCs modified with pBabe‑puro‑Snail were detected by methyl thiazolyl tetrazolium and migration assays, respectively; finally, functional recovery of SCI was assessed using the Basso, Beattie, and Bresnahan rating scales. The results showed that NRG1 concentration‑dependently promoted the expression of Snail with a peak at 40 ng/ml and 48 h; NRG1 enhanced the promoting effect of Snail on the expression of MMP‑2; the overexpression of Snail did not enhance the cell growth of the BMSCs. The NRG1‑modified BMSCs promoted the functional recovery of SCI. These results suggested that NRG1 significantly promoted the expression of MMP‑2 by upregulating the expression of Snail, and enhanced cell migration of the BMSCs conducive to the functional recovery of SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Yi XM, Chen Y and Tu GJ: Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury. Mol Med Rep 20: 41-48, 2019.
APA
Yi, X., Chen, Y., & Tu, G. (2019). Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury. Molecular Medicine Reports, 20, 41-48. https://doi.org/10.3892/mmr.2019.10217
MLA
Yi, X., Chen, Y., Tu, G."Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury". Molecular Medicine Reports 20.1 (2019): 41-48.
Chicago
Yi, X., Chen, Y., Tu, G."Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury". Molecular Medicine Reports 20, no. 1 (2019): 41-48. https://doi.org/10.3892/mmr.2019.10217
Copy and paste a formatted citation
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Spandidos Publications style
Yi XM, Chen Y and Tu GJ: Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury. Mol Med Rep 20: 41-48, 2019.
APA
Yi, X., Chen, Y., & Tu, G. (2019). Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury. Molecular Medicine Reports, 20, 41-48. https://doi.org/10.3892/mmr.2019.10217
MLA
Yi, X., Chen, Y., Tu, G."Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury". Molecular Medicine Reports 20.1 (2019): 41-48.
Chicago
Yi, X., Chen, Y., Tu, G."Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive to functional recovery following spinal cord injury". Molecular Medicine Reports 20, no. 1 (2019): 41-48. https://doi.org/10.3892/mmr.2019.10217
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