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MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40

  • Authors:
    • Hong Tan
    • Hua Yao
    • Zhenbang Lie
    • Guo Chen
    • Shuguang Lin
    • Ying Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 236-244
    |
    Published online on: May 16, 2019
       https://doi.org/10.3892/mmr.2019.10251
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Abstract

Pulmonary arterial hypertension (PAH) is a fatal and currently incurable cardiopulmonary disease. Numerous microRNAs (miRNAs) serve important roles in the development of PAH. While the expression of miR‑30a‑5p was downregulated in the lung tissue of rats in a pulmonary hypertension rat model, the expression pattern and function of miR‑30a‑5p in human PAH remain unclear. Reverse transcription quantitative polymerase chain reaction (RT‑qPCR) was used to examine miR‑30a‑5p and chitinase‑3‑like protein 1 (YKL‑40) mRNA expression levels. The expression levels of YKL‑40 and apoptosis‑associated proteins were measured by western blot analysis. Cell proliferation assays and flow cytometry analysis were performed to examine cell proliferation and apoptosis, respectively. The association between miR‑30a‑5p and YKL‑40 was determined by a luciferase reporter assay, RT‑qPCR and western blot analysis. The relative expression levels of miR‑30a‑5p in plasma were increased in patients with PAH [median=13.23 (25th percentile=6.388, 75th percentile=21.91)] compared with normal controls [median=2.25 (25th percentile=1.4, 75th percentile=3.7). The expression of miR‑30a‑5p was significantly downregulated while the protein expression of YKL‑40 was significantly upregulated in hypoxia‑induced human pulmonary artery endothelial cells (HPAECs) when compared with the hypoxia‑induced group at 0 h. miR‑30a‑5p overexpression promoted HPAEC growth and inhibited apoptosis of HPAECs under hypoxia. A miR‑30a‑5p mimic decreased the luciferase activity of a luciferase reporter construct containing YKL‑40 3’‑untranslated region and also decreased YKL‑40 protein expression. YKL‑40 overexpression partly alleviated the effects of miR‑30a‑5p upregulation on proliferation and apoptosis of HPAECs under hypoxia. In conclusion, the data indicated that miR‑30a‑5p promoted cell growth and inhibited apoptosis of HPAECs under hypoxia by targeting YKL‑40. Therefore, the miR‑30a‑5p/YKL‑40 axis may provide a potential target for the development of novel PAH therapies.
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1 

Schermuly RT, Ghofrani HA, Wilkins MR and Grimminger F: Mechanisms of disease: Pulmonary arterial hypertension. Nat Rev Cardiol. 8:443–455. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Baptista R, Meireles J, Agapito A, Castro G, da Silva AM, Shiang T, Gonçalves F, Robalo-Martins S, Nunes-Diogo A and Reis A: Pulmonary hypertension in Portugal: First data from a nationwide registry. Biomed Res Int. 2013:4895742013. View Article : Google Scholar : PubMed/NCBI

3 

Humbert M, Gerry Coghlan J and Khanna D: Early detection and management of pulmonary arterial hypertension. Eur Respir Rev. 21:306–312. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Helan M, Aravamudan B, Hartman WR, Thompson MA, Johnson BD, Pabelick CM and Prakash YS: BDNF secretion by human pulmonary artery endothelial cells in response to hypoxia. J Mol Cell Cardiol. 68:89–97. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Rupaimoole R and Slack FJ: MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 16:203–222. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Bi R, Bao C, Jiang L, Liu H, Yang Y, Mei J and Ding F: MicroRNA-27b plays a role in pulmonary arterial hypertension by modulating peroxisome proliferator-activated receptor gamma dependent Hsp90-eNOS signaling and nitric oxide production. Biochem Biophys Res Commun. 460:469–475. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Liu Y, Liu G, Zhang H and Wang J: MiRNA-199a-5p influences pulmonary artery hypertension via downregulating Smad3. Biochem Biophys Res Commun. 473:859–866. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Caruso P, MacLean MR, Khanin R, McClure J, Soon E, Southgate M, MacDonald RA, Greig JA, Robertson KE, Masson R, et al: Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline. Arterioscler Thromb Vasc Biol. 30:716–723. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Rothman AM, Chico TJ and Lawrie A: MicroRNA in pulmonary vascular disease. Prog Mol Biol Transl Sci. 124:43–63. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Wei W, Yang Y, Cai J, Cui K, Li RX, Wang H, Shang X and Wei D: MiR-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3. Cell Physiol Biochem. 39:1165–1176. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Schultz NA and Johansen JS: YKL-40-A protein in the field of translational medicine: A role as a biomarker in cancer patients? Cancers (Basel). 2:1453–1491. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Pouyafar A, Heydarabad MZ, Mahboob S, Mokhtarzadeh A and Rahbarghazi R: Angiogenic potential of YKL-40 in the dynamics of tumor niche. Biomed Pharmacother. 100:478–485. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Sun L, Wang D, Li H, et al: Significance of high YKL-40 expression regulated by mir-24 in cervical cancer progression and prognosis. Int J Clin Exp Pathol. 9:5128–5137. 2016.(In Chinese).

14 

Rathcke CN and Vestergaard H: YKL-40-an emerging biomarker in cardiovascular disease and diabetes. Cardiovasc Diabetol. 8:612009. View Article : Google Scholar : PubMed/NCBI

15 

Kastrup J: Can YKL-40 be a new inflammatory biomarker in cardiovascular disease? Immunobiology. 217:483–491. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Nojgaard C, Host NB, Christensen IJ, Poulsen SH, Egstrup K, Price PA and Johansen JS: Serum levels of YKL-40 increases in patients with acute myocardial infarction. Coron Artery Dis. 19:257–263. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Chen G, Yang T, Gu Q, Ni XH, Zhao ZH, Ye J, Meng XM, Liu ZH, He JG and Xiong CM: Elevated plasma YKL-40 as a prognostic indicator in patients with idiopathic pulmonary arterial hypertension. Respirology. 19:608–615. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Green DE, Murphy TC, Kang BY, Kleinhenz JM, Szyndralewiez C, Page P, Sutliff RL and Hart CM: The Nox4 inhibitor GKT137831 attenuates hypoxia-induced pulmonary vascular cell proliferation. Am J Respir Cell Mol Biol. 47:718–726. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Yuan T, Chen Y, Zhang H, Fang L and Du G: Salvianolic Acid A, a component of salvia miltiorrhiza, attenuates endothelial-mesenchymal transition of HPAECs Induced by Hypoxia. Am J Chin Med. 45:1185–1200. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Bienertova-Vasku J, Novak J and Vasku A: MicroRNAs in pulmonary arterial hypertension: Pathogenesis, diagnosis and treatment. J Am Soc Hypertens. 9:221–234. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Nie X, Chen Y, Tan J, Dai Y, Mao W, Qin G, Ye S, Sun J, Yang Z and Chen J: MicroRNA-221-3p promotes pulmonary artery smooth muscle cells proliferation by targeting AXIN2 during pulmonary arterial hypertension. Vascul Pharmacol. 116:24–35. 2019. View Article : Google Scholar : PubMed/NCBI

23 

Courboulin A, Paulin R, Giguere NJ, Saksouk N, Perreault T, Meloche J, Paquet ER, Biardel S, Provencher S, Côté J, et al: Role for miR-204 in human pulmonary arterial hypertension. J Exp Med. 208:535–548. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Baraniskin A, Birkenkamp-Demtroder K, Maghnouj A, Zöllner H, Munding J, Klein-Scory S, Reinacher-Schick A, Schwarte-Waldhoff I, Schmiegel W and Hahn SA: MiR-30a-5p suppresses tumor growth in colon carcinoma by targeting DTL. Carcinogenesis. 33:732–739. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Liu Y, Zhou Y, Gong X and Zhang C: MicroRNA-30a-5p inhibits the proliferation and invasion of gastric cancer cells by targeting insulin-like growth factor 1 receptor. Exp Ther Med. 14:173–180. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Chen A, Liu J, Zhu J, Wang X, Xu Z, Cui Z, Yao D, Huang Z, Xu M, Chen M, et al: FGF21 attenuates hypoxiainduced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress. Int J Mol Med. 42:1684–1694. 2018.PubMed/NCBI

27 

Yu H, Liu J, Dong Y, Xu M, Xu L, Guan H, Xia X and Wang L: Anti-hypoxic effect of dihydroartemisinin on pulmonary artery endothelial cells. Biochem Biophys Res Commun. 506:840–846. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Xu W and Erzurum SC: Endothelial cell energy metabolism, proliferation, and apoptosis in pulmonary hypertension. Compr Physiol. 1:357–372. 2011.PubMed/NCBI

29 

Fang F, Chang RM, Yu L, Lei X, Xiao S, Yang H and Yang LY: MicroRNA-188-5p suppresses tumor cell proliferation and metastasis by directly targeting FGF5 in hepatocellular carcinoma. J Hepatol. 63:874–885. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Karalilova R, Kazakova M, Batalov A and Sarafian V: Correlation between protein YKL-40 and ultrasonographic findings in active knee osteoarthritis. Med Ultrason. 1:57–63. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Tong X, Wang D, Liu S, Ma Y, Li Z, Tian P and Fan H: The YKL-40 protein is a potential biomarker for COPD: A meta-analysis and systematic review. Int J Chron Obstruct Pulmon Dis. 13:409–418. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tan H, Yao H, Lie Z, Chen G, Lin S and Zhang Y: MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40. Mol Med Rep 20: 236-244, 2019.
APA
Tan, H., Yao, H., Lie, Z., Chen, G., Lin, S., & Zhang, Y. (2019). MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40. Molecular Medicine Reports, 20, 236-244. https://doi.org/10.3892/mmr.2019.10251
MLA
Tan, H., Yao, H., Lie, Z., Chen, G., Lin, S., Zhang, Y."MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40". Molecular Medicine Reports 20.1 (2019): 236-244.
Chicago
Tan, H., Yao, H., Lie, Z., Chen, G., Lin, S., Zhang, Y."MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40". Molecular Medicine Reports 20, no. 1 (2019): 236-244. https://doi.org/10.3892/mmr.2019.10251
Copy and paste a formatted citation
x
Spandidos Publications style
Tan H, Yao H, Lie Z, Chen G, Lin S and Zhang Y: MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40. Mol Med Rep 20: 236-244, 2019.
APA
Tan, H., Yao, H., Lie, Z., Chen, G., Lin, S., & Zhang, Y. (2019). MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40. Molecular Medicine Reports, 20, 236-244. https://doi.org/10.3892/mmr.2019.10251
MLA
Tan, H., Yao, H., Lie, Z., Chen, G., Lin, S., Zhang, Y."MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40". Molecular Medicine Reports 20.1 (2019): 236-244.
Chicago
Tan, H., Yao, H., Lie, Z., Chen, G., Lin, S., Zhang, Y."MicroRNA‑30a‑5p promotes proliferation and inhibits apoptosis of human pulmonary artery endothelial cells under hypoxia by targeting YKL‑40". Molecular Medicine Reports 20, no. 1 (2019): 236-244. https://doi.org/10.3892/mmr.2019.10251
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