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Article Open Access

Substance P enhances BMSC osteogenic differentiation via autophagic activation

  • Authors:
    • Wen Geng
    • Huimin Shi
    • Ximin Zhang
    • Wei Tan
    • Yuan Cao
    • Rongcheng Mei
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China, Department of Ophthalmology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China
    Copyright: © Geng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 664-670
    |
    Published online on: May 21, 2019
       https://doi.org/10.3892/mmr.2019.10257
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Abstract

Bone mesenchymal stem cells (BMSCs) are the most commonly investigated progenitor cells in bone tissue engineering for treating severe bone defects. Strategies for regulating BMSC differentiation fate have received wide attention, in which redox homeostasis plays an important role due to the change in energy metabolism during stem cell differentiation. In the present study, it was observed that autophagic activity was induced along with BMSC osteogenic differentiation and subsequently regulated reactive oxygen species (ROS) generation and the level of osteogenesis. Furthermore, it was also observed that neuropeptide substance P (SP) administration could enhance the autophagic activity in rat BMSCs via the AMPK and mTOR pathways, as well as decreasing ROS generation and promoting osteogenic differentiation. Inhibition of autophagic activity by 3‑MA reversed the effects of SP on ROS and osteogenic levels. The present results indicated that autophagic activity participated in the regulation of differentiation fate of BMSCs and SP could promote osteogenic differentiation by activating autophagy, providing a more precise biological mechanism for its application in bone tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Geng W, Shi H, Zhang X, Tan W, Cao Y and Mei R: Substance P enhances BMSC osteogenic differentiation via autophagic activation. Mol Med Rep 20: 664-670, 2019.
APA
Geng, W., Shi, H., Zhang, X., Tan, W., Cao, Y., & Mei, R. (2019). Substance P enhances BMSC osteogenic differentiation via autophagic activation. Molecular Medicine Reports, 20, 664-670. https://doi.org/10.3892/mmr.2019.10257
MLA
Geng, W., Shi, H., Zhang, X., Tan, W., Cao, Y., Mei, R."Substance P enhances BMSC osteogenic differentiation via autophagic activation". Molecular Medicine Reports 20.1 (2019): 664-670.
Chicago
Geng, W., Shi, H., Zhang, X., Tan, W., Cao, Y., Mei, R."Substance P enhances BMSC osteogenic differentiation via autophagic activation". Molecular Medicine Reports 20, no. 1 (2019): 664-670. https://doi.org/10.3892/mmr.2019.10257
Copy and paste a formatted citation
x
Spandidos Publications style
Geng W, Shi H, Zhang X, Tan W, Cao Y and Mei R: Substance P enhances BMSC osteogenic differentiation via autophagic activation. Mol Med Rep 20: 664-670, 2019.
APA
Geng, W., Shi, H., Zhang, X., Tan, W., Cao, Y., & Mei, R. (2019). Substance P enhances BMSC osteogenic differentiation via autophagic activation. Molecular Medicine Reports, 20, 664-670. https://doi.org/10.3892/mmr.2019.10257
MLA
Geng, W., Shi, H., Zhang, X., Tan, W., Cao, Y., Mei, R."Substance P enhances BMSC osteogenic differentiation via autophagic activation". Molecular Medicine Reports 20.1 (2019): 664-670.
Chicago
Geng, W., Shi, H., Zhang, X., Tan, W., Cao, Y., Mei, R."Substance P enhances BMSC osteogenic differentiation via autophagic activation". Molecular Medicine Reports 20, no. 1 (2019): 664-670. https://doi.org/10.3892/mmr.2019.10257
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