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Article Open Access

Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts

  • Authors:
    • Yuwei Gao
    • Qiguang Zang
    • Hongquan Song
    • Songbin Fu
    • Wenjing Sun
    • Wei Zhang
    • Xiaotong Wang
    • Yong Li
    • Xiaohui Jiao
  • View Affiliations / Copyright

    Affiliations: Department of Oral Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Oral Maxillofacial Surgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 513-528
    |
    Published online on: May 22, 2019
       https://doi.org/10.3892/mmr.2019.10261
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Abstract

Non‑syndromic orofacial clefts (NSOC), which include cleft lip with or without cleft palate (CL/P) and cleft palate only (CPO), are common congenital birth defects in humans. Accumulating evidence indicates that long non‑coding RNAs (lncRNAs) and microRNAs (miRNAs or miRs) play important roles in NSOC; however, the potential regulatory associations between them remain largely unknown. In this study, we performed next‑generation RNA sequencing (RNA‑seq) to identify transcriptome profiles, including mRNAs, lncRNAs and miRNAs, in patients with CL/P and CPO. A total of 36 lncRNAs, 1,341 mRNAs and 60 miRNAs were found to be differentially expressed in the CL/P group compared to the control group, and 57 lncRNAs, 1,255 mRNAs and 162 miRNAs were found to be differentially expressed in the CPO group compared to the control group. Subsequently, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to validate the expression of selected lncRNAs, miRNAs and mRNAs. In addition, bioinformatics methods were employed to explore the potential functions of ncRNAs and to construct lncRNA‑miRNA‑mRNA regulatory networks. To the best of our knowledge, this is the first study to comprehensively analyze regulated non‑coding RNAs (ncRNAs) in CL/P and CPO, providing a novel perspective on the etiology of NSOC and laying the foundation for future research into the potential regulatory mechanisms of ncRNAs and mRNAs in NSOC.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, Zang Q, Song H, Fu S, Sun W, Zhang W, Wang X, Li Y and Jiao X: Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts. Mol Med Rep 20: 513-528, 2019.
APA
Gao, Y., Zang, Q., Song, H., Fu, S., Sun, W., Zhang, W. ... Jiao, X. (2019). Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts. Molecular Medicine Reports, 20, 513-528. https://doi.org/10.3892/mmr.2019.10261
MLA
Gao, Y., Zang, Q., Song, H., Fu, S., Sun, W., Zhang, W., Wang, X., Li, Y., Jiao, X."Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts". Molecular Medicine Reports 20.1 (2019): 513-528.
Chicago
Gao, Y., Zang, Q., Song, H., Fu, S., Sun, W., Zhang, W., Wang, X., Li, Y., Jiao, X."Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts". Molecular Medicine Reports 20, no. 1 (2019): 513-528. https://doi.org/10.3892/mmr.2019.10261
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, Zang Q, Song H, Fu S, Sun W, Zhang W, Wang X, Li Y and Jiao X: Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts. Mol Med Rep 20: 513-528, 2019.
APA
Gao, Y., Zang, Q., Song, H., Fu, S., Sun, W., Zhang, W. ... Jiao, X. (2019). Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts. Molecular Medicine Reports, 20, 513-528. https://doi.org/10.3892/mmr.2019.10261
MLA
Gao, Y., Zang, Q., Song, H., Fu, S., Sun, W., Zhang, W., Wang, X., Li, Y., Jiao, X."Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts". Molecular Medicine Reports 20.1 (2019): 513-528.
Chicago
Gao, Y., Zang, Q., Song, H., Fu, S., Sun, W., Zhang, W., Wang, X., Li, Y., Jiao, X."Comprehensive analysis of differentially expressed profiles of non‑coding RNAs in peripheral blood and ceRNA regulatory networks in non‑syndromic orofacial clefts". Molecular Medicine Reports 20, no. 1 (2019): 513-528. https://doi.org/10.3892/mmr.2019.10261
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