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Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells

  • Authors:
    • Liping Xu
    • Xuemei Lin
    • Yihua Zheng
    • Hua Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Disease, Jiangshan People's Hospital, Jiangshan, Zhejiang 324100, P.R. China, Department of Respiratory Disease, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 613-621
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    Published online on: May 22, 2019
       https://doi.org/10.3892/mmr.2019.10263
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Abstract

Radiotherapy is a useful treatment for malignant tumors, including lung carcinoma; however, non‑small cell lung carcinoma (NSCLC) is frequently insensitive to radiation. It has been reported that heat shock protein 27 (HSPB1) is a radioresistance‑associated protein in nasopharyngeal carcinoma. In the present study, the role of HSPB1 in NSCLC cells induced by irradiation was investigated. The viability of cells was determined by a Cell Counting Kit‑8 assay. The apoptotic activity, cell cycle distribution and mitochondrial membrane potential (MMP) of cells were evaluated via flow cytometry. Reverse transcription‑quantitative polymerase chain reaction and western blot analyses were employed to measure the expression of various genes and proteins. It was observed that knockdown of HSPB1 with small interfering RNA (si‑HSPB1) markedly decreased the viability of A549 NSCLC cells and induced cell cycle arrest in the G2/M phase following exposure to 6 Gy irradiation. Furthermore, it was revealed that si‑HSPB1 significantly downregulated cyclin B1 and cyclin G1 expression. Additionally, si‑HSPB1 promoted apoptosis and depolarized the MMP of cells exposed to 6 Gy irradiation. The expression levels of B‑cell lymphoma‑2 (Bcl‑2), mitochondrial cytochrome c (cyto c) and pro‑caspase‑8 were downregulated, whereas those of Bcl‑2 associated X protein (Bax), cytosolic cyto c and cleaved‑caspase‑8 were upregulated. Collectively, silencing of HSPB1 increased the radiosensitivity of NSCLC cells by reducing cell viability, depolarizing the MMP, arresting the cell cycle in the G2/M phase and promoting cell apoptosis. Therefore, HSPB1 may be a novel target for increasing radiosensitivity in the treatment of NSCLC.
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Spandidos Publications style
Xu L, Lin X, Zheng Y and Zhou H: Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells. Mol Med Rep 20: 613-621, 2019.
APA
Xu, L., Lin, X., Zheng, Y., & Zhou, H. (2019). Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells. Molecular Medicine Reports, 20, 613-621. https://doi.org/10.3892/mmr.2019.10263
MLA
Xu, L., Lin, X., Zheng, Y., Zhou, H."Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells". Molecular Medicine Reports 20.1 (2019): 613-621.
Chicago
Xu, L., Lin, X., Zheng, Y., Zhou, H."Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells". Molecular Medicine Reports 20, no. 1 (2019): 613-621. https://doi.org/10.3892/mmr.2019.10263
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Spandidos Publications style
Xu L, Lin X, Zheng Y and Zhou H: Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells. Mol Med Rep 20: 613-621, 2019.
APA
Xu, L., Lin, X., Zheng, Y., & Zhou, H. (2019). Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells. Molecular Medicine Reports, 20, 613-621. https://doi.org/10.3892/mmr.2019.10263
MLA
Xu, L., Lin, X., Zheng, Y., Zhou, H."Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells". Molecular Medicine Reports 20.1 (2019): 613-621.
Chicago
Xu, L., Lin, X., Zheng, Y., Zhou, H."Silencing of heat shock protein 27 increases the radiosensitivity of non‑small cell lung carcinoma cells". Molecular Medicine Reports 20, no. 1 (2019): 613-621. https://doi.org/10.3892/mmr.2019.10263
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