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Article Open Access

MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways

  • Authors:
    • Huixiao Dong
    • Benliang Cui
    • Xiuzhen Hao
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Shandong Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China, Department of the Third Surgery, Shandong Jining Traditional Chinese Medicine Hospital, Jining, Shandong 272000, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 735-744
    |
    Published online on: May 22, 2019
       https://doi.org/10.3892/mmr.2019.10269
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Abstract

The present study aimed to ascertain the potential roles and mechanisms of action of micro (mi)RNA‑22 in ischemic stroke. The results indicated that miRNA‑22 expression was downregulated in ischemic stroke rats model, compared with a control group. The downregulation of miRNA‑22 upregulated the expression of inflammatory factors [including tumor necrosis factor‑α, interleukin (IL)‑1β, IL‑6 and IL‑18]. It could also induce the expression of macrophage inflammatory protein (MIP‑2), prostaglandin E2 (PGE2), cyclooxygenase‑2 (COX‑2) and inducible NO synthase (iNOS) in the in vitro model. By contrast, the overexpression of miRNA‑22 downregulated the expression of inflammatory factors, and suppressed the expression of MIP‑2, PGE2, COX‑2 and iNOS in the in vitro model. The downregulation of miRNA‑22 induced the protein expression of nuclear factor (NF)‑κB and phosphorylated‑p38 (p‑p38) mitogen‑activated protein kinase (MAPK) in the in vitro model. By comparison, the overexpression of miRNA‑22 suppressed the protein expression of NF‑κB and p‑p38 in the in vitro model. Typically, LY2228820, the p38 inhibitor (3 nM) would mitigate the pro‑inflammatory effects of anti‑miRNA‑22 in the in vitro model. These results suggested that miRNA‑22 can alleviate ischemic stroke‑induced inflammation in rats model or vitro model through p38 MAPK/NF‑κB pathway suppression.
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Copy and paste a formatted citation
Spandidos Publications style
Dong H, Cui B and Hao X: MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways. Mol Med Rep 20: 735-744, 2019.
APA
Dong, H., Cui, B., & Hao, X. (2019). MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways. Molecular Medicine Reports, 20, 735-744. https://doi.org/10.3892/mmr.2019.10269
MLA
Dong, H., Cui, B., Hao, X."MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways". Molecular Medicine Reports 20.1 (2019): 735-744.
Chicago
Dong, H., Cui, B., Hao, X."MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways". Molecular Medicine Reports 20, no. 1 (2019): 735-744. https://doi.org/10.3892/mmr.2019.10269
Copy and paste a formatted citation
x
Spandidos Publications style
Dong H, Cui B and Hao X: MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways. Mol Med Rep 20: 735-744, 2019.
APA
Dong, H., Cui, B., & Hao, X. (2019). MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways. Molecular Medicine Reports, 20, 735-744. https://doi.org/10.3892/mmr.2019.10269
MLA
Dong, H., Cui, B., Hao, X."MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways". Molecular Medicine Reports 20.1 (2019): 735-744.
Chicago
Dong, H., Cui, B., Hao, X."MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways". Molecular Medicine Reports 20, no. 1 (2019): 735-744. https://doi.org/10.3892/mmr.2019.10269
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