Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2019 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2019 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells

  • Authors:
    • Maria Billert
    • Maciej Sassek
    • Tatiana Wojciechowicz
    • Mariami Jasaszwili
    • Mathias Z. Strowski
    • Krzysztof W. Nowak
    • Marek Skrzypski
  • View Affiliations / Copyright

    Affiliations: Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, 60‑637 Poznań, Poland, Department of Hepatology and Gastroenterology, Charité‑University Medicine Berlin, D‑13353 Berlin, Germany
  • Pages: 2030-2038
    |
    Published online on: June 24, 2019
       https://doi.org/10.3892/mmr.2019.10415
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Neuropeptide B (NPB) regulates food intake, body weight and energy homeostasis by interacting with NPBW1/NPBW2 in humans and NPBW1 in rodents. NPB and NPBW1 are widely expressed in the central nervous system and peripheral tissues including pancreatic islets. Although previous studies have demonstrated a prominent role for NPB and NPBW1 in controlling glucose and energy homeostasis, it remains unknown as to whether NPB modulates pancreatic β‑cell functions. Therefore, the aim of the present study was to investigate the effects of NPB on insulin expression and secretion in vitro. Furthermore, the role of NPB in the modulation of INS‑1E cell growth, viability and death was examined. Gene expression was assessed by reverse transcription‑quantitative PCR. Cell proliferation and viability were determined by BrdU or MTT tests, respectively. Apoptotic cell death was evaluated by relative quantification histone‑complexed DNA fragments (mono‑and oligonucleosomes). Insulin secretion was studied using an ELISA test. Protein phosphorylation was assessed by western blot analysis. NPB and NPBW1 mRNA was expressed in INS‑1E cells and rat pancreatic islets. In INS‑1E cells, NPB enhanced insulin 1 mRNA expression via an ERK1/2‑dependent mechanism. Furthermore, NPB stimulated insulin secretion from INS‑1E cells and rat pancreatic islets. By contrast, NPB failed to affect INS‑1E cell growth or death. We conclude that NPB may regulate insulin secretion and expression in INS‑1E cells and insulin secretion in rat pancreatic islets.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Tanaka H, Yoshida T, Miyamoto N, Motoike T, Kurosu H, Shibata K, Yamanaka A, Williams SC, Richardson JA, Tsujino N, et al: Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8. Proc Natl Acad Sci USA. 100:6251–6256. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Fujii R, Yoshida H, Fukusumi S, Habata Y, Hosoya M, Kawamata Y, Yano T, Hinuma S, Kitada C, Asami T, et al: Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7. J Biol Chem. 277:34010–34016. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Lee DK, Nguyen T, Porter CA, Cheng R, George SR and O'Dowd BF: Two related G protein-coupled receptors: The distribution of GPR7 in rat brain and the absence of GPR8 in rodents. Brain Res Mol Brain Res. 71:96–103. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Chottova Dvorakova M: Distribution and function of neuropeptides W/B signaling system. Front Physiol. 9:9812018. View Article : Google Scholar : PubMed/NCBI

5 

Yamamoto T, Saito O, Shono K and Tanabe S: Anti-hyperalgesic effects of intrathecally administered neuropeptide W-23, and neuropeptide B, in tests of inflammatory pain in rats. Brain Res. 1045:97–106. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Aikawa S, Ishii M, Yanagisawa M, Sakakibara Y and Sakurai T: Effect of neuropeptide B on feeding behavior is influenced by endogenous corticotropin-releasing factor activities. Regul Pept. 151:147–152. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Uchio N, Doi M, Matsuo M, Yamazaki F, Mizoro Y, Hondo M, Sakurai T and Okamura H: Circadian characteristics of mice depleted with GPR7. Biomed Res. 30:357–364. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Hochol A, Belloni AS, Rucinski M, Ziolkowska A, Di Liddo R, Nussdorfer GG and Malendowicz LK: Expression of neuropeptides B and W and their receptors in endocrine glands of the rat. Int J Mol Med. 18:1101–1106. 2006.PubMed/NCBI

9 

Ishii M, Fei H and Friedman JM: Targeted disruption of GPR7, the endogenous receptor for neuropeptides B and W, leads to metabolic defects and adult-onset obesity. Proc Natl Acad Sci USA. 100:10540–10545. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Kelly MA, Beuckmann CT, Williams SC, Sinton CM, Motoike T, Richardson JA, Hammer RE, Garry MG and Yanagisawa M: Neuropeptide B-deficient mice demonstrate hyperalgesia in response to inflammatory pain. Proc Natl Acad Sci USA. 102:9942–9947. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Skrzypski M, Pruszynska-Oszmalek E, Rucinski M, Szczepankiewicz D, Sassek M, Wojciechowicz T, Kaczmarek P, Kołodziejski PA, Strowski MZ, Malendowicz LK and Nowak KW: Neuropeptide B and W regulate leptin and resistin secretion, and stimulate lipolysis in isolated rat adipocytes. Regul Pept. 176:51–56. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Grzelak T, Tyszkiewicz-Nwafor M, Dutkiewicz A, Mikulska AA, Dmitrzak-Weglarz M, Slopien A, Czyzewska K and Paszynska E: Neuropeptide B and vaspin as new biomarkers in anorexia nervosa. Biomed Res Int. 2018:97275092018. View Article : Google Scholar : PubMed/NCBI

13 

Grzelak T, Wedrychowicz A, Grupinska J, Grupinska J, Pelczynska M, Sperling M, Mikulska AA, Naughton V and Czyzewska K: Neuropeptide B and neuropeptide W as new serum predictors of nutritional status and of clinical outcomes in pediatric patients with type 1 diabetes mellitus treated with the use of pens or insulin pumps. Arch Med Scie. 15:619–631. 2019. View Article : Google Scholar

14 

Fu Z, Gilbert ER and Liu D: Regulation of insulin synthesis and secretion and pancreatic Beta-cell dysfunction in diabetes. Curr Diabetes Rev. 9:25–53. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Klöppel G, Löhr M, Habich K, Oberholzer M and Heitz PU: Islet pathology and the pathogenesis of type 1 and type 2 diabetes mellitus revisited. Surv Synth Pathol Res. 4:110–125. 1985.PubMed/NCBI

16 

Porte D Jr and Kahn SE: Beta-cell dysfunction and failure in type 2 diabetes: Potential mechanisms. Diabetes. 50 (Suppl 1):S160–S163. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Sakurai T: NPBWR1 and NPBWR2: Implications in energy homeostasis, pain, and emotion. Front Endocrinol (Lausanne). 4:232013. View Article : Google Scholar : PubMed/NCBI

18 

Merglen A, Theander S, Rubi B, Chaffard G, Wollheim CB and Maechler P: Glucose sensitivity and metabolism-secretion coupling studied during two-year continuous culture in INS-1E insulinoma cells. Endocrinology. 145:667–678. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Billert M, Skrzypski M, Sassek M, Szczepankiewicz D, Wojciechowicz T, Mergler S, Strowski MZ and Nowak KW: TRPV4 regulates insulin mRNA expression and INS-1E cell death via ERK1/2 and NO-dependent mechanisms. Cell Signal. 35:242–249. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Mazzocchi G, Rebuffat P, Ziolkowska A, Rossi GP, Malendowicz LK and Nussdorfer GG: G protein receptors 7 and 8 are expressed in human adrenocortical cells, and their endogenous ligands neuropeptides B and w enhance cortisol secretion by activating adenylate cyclase- and phospholipase C-dependent signaling cascades. J Clin Endocrinol Metab. 90:3466–3471. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Khoo S, Gibson TB, Arnette D, Lawrence M, January B, McGlynn K, Vanderbilt CA, Griffen SC, German MS and Cobb MH: MAP kinases and their roles in pancreatic beta-cells. Cell Biochem Biophys 40 (3 Suppl). S191–S200. 2004. View Article : Google Scholar

22 

Favata MF, Horiuchi KY, Manos EJ, Daulerio AJ, Stradley DA, Feeser WS, Van Dyk DE, Pitts WJ, Earl RA, Hobbs F, et al: Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J Biol Chem. 273:18623–18632. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Dezaki K, Kageyama H, Seki M, Shioda S and Yada T: Neuropeptide W in the rat pancreas: Potentiation of glucose-induced insulin release and Ca2+ influx through L-type Ca2+ channels in beta-cells and localization in islets. Regul Pept. 145:153–158. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Andrali SS, Sampley ML, Vanderford NL and Ozcan S: Glucose regulation of insulin gene expression in pancreatic beta-cells. Biochem J. 415:1–10. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Seufert J, Kieffer TJ and Habener JF: Leptin inhibits insulin gene transcription and reverses hyperinsulinemia in leptin-deficient ob/ob mice. Proc Natl Acad Sci USA. 96:674–679. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Colombo M, Gregersen S, Xiao J and Hermansen K: Effects of ghrelin and other neuropeptides (CART, MCH, orexin A and B, and GLP-1) on the release of insulin from isolated rat islets. Pancreas. 27:161–166. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Skrzypski M, Khajavi N, Mergler S, Billert M, Szczepankiewicz D, Wojciechowicz T, Nowak KW and Strowski MZ: Orexin A modulates INS-1E cell proliferation and insulin secretion via extracellular signal-regulated kinase and transient receptor potential channels. J Physiol Pharmacol. 67:643–652. 2016.PubMed/NCBI

28 

Böhm SK, Grady EF and Bunnett NW: Regulatory mechanisms that modulate signalling by G-protein-coupled receptors. Biochem J. 322:1–18. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Yoon JC, Xu G, Deeney JT, Yang SN, Rhee J, Puigserver P, Levens AR, Yang R, Zhang CY, Lowell BB, et al: Suppression of beta cell energy metabolism and insulin release by PGC-1alpha. Dev Cell. 5:73–83. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Wang H, Maechler P, Antinozzi PA, Hagenfeldt KA and Wollheim CB: Hepatocyte nuclear factor 4alpha regulates the expression of pancreatic beta-cell genes implicated in glucose metabolism and nutrient-induced insulin secretion. J Biol Chem. 275:35953–35959. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Kataoka K, Han SI, Shioda S, Hirai M, Nishizawa M and Handa H: MafA is a glucose-regulated and pancreatic beta-cell-specific transcriptional activator for the insulin gene. J Biol Chem. 277:49903–49910. 2002. View Article : Google Scholar : PubMed/NCBI

32 

De Vos A, Heimberg H, Quartier E, Huypens P, Bouwens L, Pipeleers D and Schuit F: Human and rat beta cells differ in glucose transporter but not in glucokinase gene expression. J Clin Invest. 96:2489–2495. 1995. View Article : Google Scholar : PubMed/NCBI

33 

Lawrence MC, McGlynn K, Park BH and Cobb MH: ERK1/2-dependent activation of transcription factors required for acute and chronic effects of glucose on the insulin gene promoter. J Biol Chem. 280:26751–26759. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Andreis PG, Rucinski M, Neri G, Conconi MT, Petrelli L, Parnigotto PP, Malendowicz LK and Nussdorfer GG: Neuropeptides B and W enhance the growth of human adrenocortical carcinoma-derived NCI-H295 cells by exerting MAPK p42/p44-mediated proliferogenic and antiapoptotic effects. Int J Mol Med. 16:1021–1028. 2005.PubMed/NCBI

35 

Deltour L, Leduque P, Blume N, Madsen O, Dubois P, Jami J and Bucchini D: Differential expression of the two nonallelic proinsulin genes in the developing mouse embryo. Proc Natl Acad Sci USA. 90:527–531. 1993. View Article : Google Scholar : PubMed/NCBI

36 

Roderigo-Milne H, Hauge-Evans AC, Persaud SJ and Jones PM: Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets. Biochem Biophys Res Commun. 296:589–595. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Gu C, Stein GH, Pan N, Goebbels S, Hörnberg H, Nave KA, Herrera P, White P, Kaestner KH, Sussel L and Lee JE: Pancreatic beta cells require NeuroD to achieve and maintain functional maturity. Cell Metab. 11:298–310. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Nishimura W, Takahashi S and Yasuda K: MafA is critical for maintenance of the mature beta cell phenotype in mice. Diabetologia. 58:566–574. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Brezillon S, Lannoy V, Franssen JD, Le Poul E, Dupriez V, Lucchetti J, Detheux M and Parmentier M: Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8. J Biol Chem. 278:776–783. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Cnop M, Welsh N, Jonas JC, Jorns A, Lenzen S and Eizirik DL: Mechanisms of pancreatic beta-cell death in type 1 and type 2 diabetes: Many differences, few similarities. Diabetes. 54 (Suppl 2):S97–S107. 2005. View Article : Google Scholar : PubMed/NCBI

41 

Chen C, Cohrs CM, Stertmann J, Bozsak R and Speier S: Human beta cell mass and function in diabetes: Recent advances in knowledge and technologies to understand disease pathogenesis. Mol Metab. 6:943–957. 2017. View Article : Google Scholar : PubMed/NCBI

42 

Ziolkowska A, Rucinski M, Tyczewska M and Malendowicz LK: Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells. Int J Mol Med. 24:781–787. 2009.PubMed/NCBI

43 

Hochol A, Albertin G, Nussdorfer GG, Spinazzi R, Ziolkowska A, Rucinski M and Malendowicz LK: Effects of neuropeptides B and W on the secretion and growth of rat adrenocortical cells. Int J Mol Med. 14:843–847. 2004.PubMed/NCBI

44 

Steiner DJ, Kim A, Miller K and Hara M: Pancreatic islet plasticity: Interspecies comparison of islet architecture and composition. Islets. 2:135–145. 2010. View Article : Google Scholar : PubMed/NCBI

45 

Redmon JB, Towle HC and Robertson RP: Regulation of human insulin gene transcription by glucose, epinephrine, and somatostatin. Diabetes. 43:546–551. 1994. View Article : Google Scholar : PubMed/NCBI

46 

Hiriart M and Aguilar-Bryan L: Channel regulation of glucose sensing in the pancreatic beta-cell. Am J Physiol Endocrinol Metab. 295:E1298–E1306. 2008. View Article : Google Scholar : PubMed/NCBI

47 

Skrzypski M, Billert M, Mergler S, Khajavi N, Nowak KW and Strowski MZ: Role of TRPV channels in regulating various pancreatic β-cell functions: Lessons from in vitro studies. Biosci Trends. 11:9–15. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Billert M, Sassek M, Wojciechowicz T, Jasaszwili M, Strowski MZ, Nowak KW and Skrzypski M: Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells. Mol Med Rep 20: 2030-2038, 2019.
APA
Billert, M., Sassek, M., Wojciechowicz, T., Jasaszwili, M., Strowski, M.Z., Nowak, K.W., & Skrzypski, M. (2019). Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells. Molecular Medicine Reports, 20, 2030-2038. https://doi.org/10.3892/mmr.2019.10415
MLA
Billert, M., Sassek, M., Wojciechowicz, T., Jasaszwili, M., Strowski, M. Z., Nowak, K. W., Skrzypski, M."Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells". Molecular Medicine Reports 20.2 (2019): 2030-2038.
Chicago
Billert, M., Sassek, M., Wojciechowicz, T., Jasaszwili, M., Strowski, M. Z., Nowak, K. W., Skrzypski, M."Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells". Molecular Medicine Reports 20, no. 2 (2019): 2030-2038. https://doi.org/10.3892/mmr.2019.10415
Copy and paste a formatted citation
x
Spandidos Publications style
Billert M, Sassek M, Wojciechowicz T, Jasaszwili M, Strowski MZ, Nowak KW and Skrzypski M: Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells. Mol Med Rep 20: 2030-2038, 2019.
APA
Billert, M., Sassek, M., Wojciechowicz, T., Jasaszwili, M., Strowski, M.Z., Nowak, K.W., & Skrzypski, M. (2019). Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells. Molecular Medicine Reports, 20, 2030-2038. https://doi.org/10.3892/mmr.2019.10415
MLA
Billert, M., Sassek, M., Wojciechowicz, T., Jasaszwili, M., Strowski, M. Z., Nowak, K. W., Skrzypski, M."Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells". Molecular Medicine Reports 20.2 (2019): 2030-2038.
Chicago
Billert, M., Sassek, M., Wojciechowicz, T., Jasaszwili, M., Strowski, M. Z., Nowak, K. W., Skrzypski, M."Neuropeptide B stimulates insulin secretion and expression but not proliferation in rat insulin‑producing INS‑1E cells". Molecular Medicine Reports 20, no. 2 (2019): 2030-2038. https://doi.org/10.3892/mmr.2019.10415
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team