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miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase

  • Authors:
    • Chunli Xu
    • Chen Wang
    • Qiuyu Meng
    • Yuming Gu
    • Qiwei Wang
    • Wenjie Xu
    • Ying Han
    • Yong Qin
    • Jiao Li
    • Song Jia
    • Jie Xu
    • Yixin Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China, School of Life Science and Technology, School of Medicine, Tongji University, Shanghai 200092, P.R. China, Teaching Laboratory Center of Medicine and Life Science, School of Medicine, Tongji University, Shanghai 200092, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1725-1735
    |
    Published online on: June 25, 2019
       https://doi.org/10.3892/mmr.2019.10421
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Abstract

MicroRNAs (miRNAs) have been found to play important regulatory roles in certain neurodegenerative diseases. The aim of the present study was to investigate the effect of miRNA‑153 (miR‑153) on the neural differentiation of HT‑22 cells. Overexpression of miR‑153 induced the differentiation of HT‑22 cells, increasing the number of protrusions and branches, reducing the S phase distribution of the cell cycle, and attenuating the cell proliferation rate as determined using the Cell Counting Kit‑8 assay. Furthermore, miR‑153 increased the expression of neuron‑specific γ‑enolase (NSE), neuronal nuclei (NeuN), and N‑ethylmaleimide‑sensitive fusion attachment protein 23 (SNAP23) and SNAP25 at the transcriptional and protein level by PCR and western blot analysis. Moreover, miR‑153 caused obvious upregulation of peroxiredoxin 5 (PRX5), which has been found to protect neural cells from death and apoptosis. miR‑153 promoted neural differentiation and protected neural cells by upregulating the neuron markers γ‑enolase, neuronal nuclei, and the functional proteins SNAP23, SNAP25 and PRX5. Therefore, miR‑153 may be a potential target for the treatment of certain neurodegenerative diseases.
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1 

Choi PS, Zakhary L, Choi WY, Caron S, Alvarez-Saavedra E, Miska EA, McManus M, Harfe B, Giraldez AJ, Horvitz RH, et al: Members of the miRNA-200 family regulate olfactory neurogenesis. Neuron. 57:41–55. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Makeyev EV, Zhang J, Carrasco MA and Maniatis T: The MicroRNA miR-124 promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing. Mol Cell. 27:435–448. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Kim J, Inoue K, Ishii J, Vanti WB, Voronov SV, Murchison E, Hannon G and Abeliovich A: A MicroRNA feedback circuit in midbrain dopamine neurons. Science. 317:1220–1224. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Haramati S, Chapnik E, Sztainberg Y, Eilam R, Zwang R, Gershoni N, McGlinn E, Heiser PW, Wills AM, Wirguin I, et al: miRNA malfunction causes spinal motor neuron disease. Proc Natl Acad Sci USA. 107:13111–13116. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Harraz MM, Dawson TM and Dawson VL: MicroRNAs in Parkinson's disease. J Chem Neuroanat. 42:127–130. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Morgado AL, Rodrigues CM and Sola S: MicroRNA-145 regulates neural stem cell differentiation through the Sox2-Lin28/let-7 signaling pathway. Stem Cells. 34:1386–1395. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Cheng LC, Pastrana E, Tavazoie M and Doetsch F: miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche. Nat Neurosci. 12:399–408. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Shibata M, Nakao H, Kiyonari H, Abe T and Aizawa S: MicroRNA-9 regulates neurogenesis in mouse telencephalon by targeting multiple transcription factors. J Neurosci. 31:3407–3422. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Doxakis E: Post-transcriptional regulation of alpha-synuclein expression by mir-7 and mir-153. J Biol Chem. 285:12726–12734. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Wei C, Thatcher EJ, Olena AF, Cha DJ, Perdigoto AL, Marshall AF, Carter BD, Broadie K and Patton JG: miR-153 regulates SNAP-25, synaptic transmission, and neuronal development. PLoS One. 8:e570802013. View Article : Google Scholar : PubMed/NCBI

11 

Tsuyama J, Bunt J, Richards LJ, Iwanari H, Mochizuki Y, Hamakubo T, Shimazaki T and Okano H: MicroRNA-153 regulates the acquisition of gliogenic competence by neural stem cells. Stem Cell Reports. 5:365–377. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Zylbersztejn K and Galli T: Vesicular traffic in cell navigation. FEBS J. 278:4497–4505. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Kim B, Park J, Chang KT and Lee DS: Peroxiredoxin 5 prevents amyloid-beta oligomer-induced neuronal cell death by inhibiting ERK-Drp1-mediated mitochondrial fragmentation. Free Radic Biol Med. 90:184–194. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

O'Brien RJ and Wong PC: Amyloid precursor protein processing and Alzheimer's disease. Annu Rev Neurosci. 34:185–204. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Qiu L, Tan EK and Zeng L: microRNAs and neurodegenerative diseases. Adv Exp Med Biol. 888:85–105. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Leggio L, Vivarelli S, L'Episcopo F, Tirolo C, Caniglia S, Testa N, Marchetti B and Iraci N: microRNAs in Parkinson's disease: From pathogenesis to novel diagnostic and therapeutic approaches. Int J Mol Sci. 18:E26982017. View Article : Google Scholar : PubMed/NCBI

18 

Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E and Ambros V: Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation. Genome Biol. 5:R132004. View Article : Google Scholar : PubMed/NCBI

19 

Bak M, Silahtaroglu A, Moller M, Christensen M, Rath MF, Skryabin B, Tommerup N and Kauppinen S: MicroRNA expression in the adult mouse central nervous system. RNA. 14:432–444. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, et al: A mammalian microRNA expression atlas based on small RNA library sequencing. Cell. 129:1401–1414. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Liu J, Li L and Suo WZ: HT22 hippocampal neuronal cell line possesses functional cholinergic properties. Life Sci. 84:267–271. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Lei T, Zhang P, Zhang X, Xiao X, Zhang J, Qiu T, Dai Q, Zhang Y, Min L, Li Q, et al: Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation. Nat Commun. 9:18762018. View Article : Google Scholar : PubMed/NCBI

23 

Juan HC, Lin Y, Chen HR and Fann MJ: Cdk12 is essential for embryonic development and the maintenance of genomic stability. Cell Death Differ. 23:1038–1048. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Chen HR, Juan HC, Wong YH, Tsai JW and Fann MJ: Cdk12 regulates neurogenesis and late-arising neuronal migration in the developing cerebral cortex. Cereb Cortex. 27:2289–2302. 2017.PubMed/NCBI

25 

Isgro MA, Bottoni P and Scatena R: Neuron-specific enolase as a biomarker: Biochemical and clinical aspects. Adv Exp Med Biol. 867:125–143. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Corradini I, Verderio C, Sala M, Wilson MC and Matteoli M: SNAP-25 in neuropsychiatric disorders. Ann N Y Acad Sci. 1152:93–99. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Scarr E, Gray L, Keriakous D, Robinson PJ and Dean B: Increased levels of SNAP-25 and synaptophysin in the dorsolateral prefrontal cortex in bipolar I disorder. Bipolar Disord. 8:133–143. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M and Li JY: Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease. J Neurochem. 103:115–123. 2007.PubMed/NCBI

29 

Bereczki E, Francis PT, Howlett D, Pereira JB, Hoglund K, Bogstedt A, Cedazo-Minguez A, Baek JH, Hortobagyi T, Attems J, et al: Synaptic proteins predict cognitive decline in Alzheimer's disease and Lewy body dementia. Alzheimers Dement. 12:1149–1158. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu C, Wang C, Meng Q, Gu Y, Wang Q, Xu W, Han Y, Qin Y, Li J, Jia S, Jia S, et al: miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase. Mol Med Rep 20: 1725-1735, 2019.
APA
Xu, C., Wang, C., Meng, Q., Gu, Y., Wang, Q., Xu, W. ... Zhou, Y. (2019). miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase. Molecular Medicine Reports, 20, 1725-1735. https://doi.org/10.3892/mmr.2019.10421
MLA
Xu, C., Wang, C., Meng, Q., Gu, Y., Wang, Q., Xu, W., Han, Y., Qin, Y., Li, J., Jia, S., Xu, J., Zhou, Y."miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase". Molecular Medicine Reports 20.2 (2019): 1725-1735.
Chicago
Xu, C., Wang, C., Meng, Q., Gu, Y., Wang, Q., Xu, W., Han, Y., Qin, Y., Li, J., Jia, S., Xu, J., Zhou, Y."miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase". Molecular Medicine Reports 20, no. 2 (2019): 1725-1735. https://doi.org/10.3892/mmr.2019.10421
Copy and paste a formatted citation
x
Spandidos Publications style
Xu C, Wang C, Meng Q, Gu Y, Wang Q, Xu W, Han Y, Qin Y, Li J, Jia S, Jia S, et al: miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase. Mol Med Rep 20: 1725-1735, 2019.
APA
Xu, C., Wang, C., Meng, Q., Gu, Y., Wang, Q., Xu, W. ... Zhou, Y. (2019). miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase. Molecular Medicine Reports, 20, 1725-1735. https://doi.org/10.3892/mmr.2019.10421
MLA
Xu, C., Wang, C., Meng, Q., Gu, Y., Wang, Q., Xu, W., Han, Y., Qin, Y., Li, J., Jia, S., Xu, J., Zhou, Y."miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase". Molecular Medicine Reports 20.2 (2019): 1725-1735.
Chicago
Xu, C., Wang, C., Meng, Q., Gu, Y., Wang, Q., Xu, W., Han, Y., Qin, Y., Li, J., Jia, S., Xu, J., Zhou, Y."miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase". Molecular Medicine Reports 20, no. 2 (2019): 1725-1735. https://doi.org/10.3892/mmr.2019.10421
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