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Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase

  • Authors:
    • Xiangjun Zhou
    • Mengmou Hu
    • Zhenghui Ge
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Danyang People's Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, P.R. China, Department of Clinical Laboratory, Danyang People's Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2151-2158
    |
    Published online on: June 27, 2019
       https://doi.org/10.3892/mmr.2019.10436
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Abstract

Gastric cancer (GC) remains a leading cause of cancer‑associated mortality globally. Emerging evidence suggests that microRNAs (miRs) function as oncogenes or tumor suppressors, contributing to various aspects of cancer progression, including invasion and metastasis. In the present study, the specific role of miR‑299‑3p in the invasion of GC cells was investigated. The expression level of miR‑299‑3p was measured using reverse transcription‑quantitative PCR and in situ hybridization in human GC tissues. Effects of miR‑299‑3p on GC cell invasion were determined by Transwell assay. Bioinformatics and luciferase reporter assays were performed to identify and verify the downstream effectors of miR‑299‑3p. miR‑299‑3p expression analysis in clinical GC samples revealed a significant downregulation of miR‑299‑3p compared with non‑tumor tissues. Inhibition of miR‑299‑3p promoted the invasive abilities of GC cells, whereas its overexpression significantly suppressed cell invasion. Bioinformatics and luciferase reporter assays identified heparanase (HPSE) as a direct target of miR‑299‑3p, the ectopic expression of which reversed the impairment in cell invasion induced by miR‑299‑3p upregulation. Furthermore, HPSE expression was negatively associated with miR‑299‑3p levels in human GC tissues. Overall, the present study indicated that miR‑299‑3p functions as a tumor suppressor by directly targeting HPSE, highlighting its potential as a target for the treatment of GC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou X, Hu M and Ge Z: Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase. Mol Med Rep 20: 2151-2158, 2019.
APA
Zhou, X., Hu, M., & Ge, Z. (2019). Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase. Molecular Medicine Reports, 20, 2151-2158. https://doi.org/10.3892/mmr.2019.10436
MLA
Zhou, X., Hu, M., Ge, Z."Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase". Molecular Medicine Reports 20.3 (2019): 2151-2158.
Chicago
Zhou, X., Hu, M., Ge, Z."Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase". Molecular Medicine Reports 20, no. 3 (2019): 2151-2158. https://doi.org/10.3892/mmr.2019.10436
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou X, Hu M and Ge Z: Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase. Mol Med Rep 20: 2151-2158, 2019.
APA
Zhou, X., Hu, M., & Ge, Z. (2019). Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase. Molecular Medicine Reports, 20, 2151-2158. https://doi.org/10.3892/mmr.2019.10436
MLA
Zhou, X., Hu, M., Ge, Z."Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase". Molecular Medicine Reports 20.3 (2019): 2151-2158.
Chicago
Zhou, X., Hu, M., Ge, Z."Tumor‑suppressive miR‑299‑3p inhibits gastric cancer cell invasion by targeting heparanase". Molecular Medicine Reports 20, no. 3 (2019): 2151-2158. https://doi.org/10.3892/mmr.2019.10436
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