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Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice

  • Authors:
    • Nancy Machorro‑Rojas
    • Teresita Sainz‑Espuñes
    • Marycarmen Godínez‑Victoria
    • Jorge Ismael Castañeda‑Sánchez
    • Rafael Campos‑Rodríguez
    • Judith Pacheco‑Yepez
    • Maria Elisa Drago‑Serrano
  • View Affiliations / Copyright

    Affiliations: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico City 04960, Mexico, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City 11340, Mexico
    Copyright: © Machorro‑Rojas et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Pages: 2083-2090
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    Published online on: June 27, 2019
       https://doi.org/10.3892/mmr.2019.10437
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Abstract

The intestinal epithelium is a monolayer of cells arranged side‑by‑side and connected by tight junction (TJ) proteins expressed at the apical extreme of the paracellular membrane. This layer prevents stress‑induced inflammatory responses, thus helping to maintain gut barrier function and gut homeostasis. The aim of the present study was to evaluate the effects of chronic immobilization stress on the colonic expression of various parameters of homeostasis. A total of two groups of female BALB/c mice (n=6) were included: A stressed group (short‑term immobilization for 2 h/day for 4 consecutive days) and an unstressed (control) group. Colon samples were obtained to detect neutrophils and goblet cells by optical microscopy, TJ protein expression (occludin, and claudin ‑2, ‑4, ‑7, ‑12 and ‑15) by western blotting, mRNA levels of TJ genes and proinflammatory cytokines [tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, ‑6 and ‑8] by reverse transcription‑quantitative PCR, fecal lactoferrin by ELISA and the number of colony‑forming units of aerobic bacteria. Compared with goblet cells in control mice, goblet cells were enlarged and reduced in number in stressed mice, whereas neutrophil cellularity was unaltered. Stressed mice exhibited reduced mRNA expression for all evaluated TJ mRNAs, with the exception of claudin‑7, which was upregulated. Protein levels of occludin and all claudins (with the exception of claudin‑12) were decreased in stressed mice. Fecal lactoferrin, proinflammatory cytokine mRNA levels and aerobic bacterial counts were all increased in the stressed group. These results indicated that immobilization stress induced proinflammatory and potential remodeling effects in the colon by decreasing TJ protein expression. The present study may be a useful reference for therapies aiming to regulate the effects of stress on intestinal inflammatory dysfunction.
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View References

1 

Zhang K, Hornef MW and Dupont A: The intestinal epithelium as guardian of gut barrier integrity. Cell Microbiol. 17:1561–1569. 2015. View Article : Google Scholar : PubMed/NCBI

2 

France MM and Turner JR: The mucosal barrier at a glance. J Cell Sci. 130:307–314. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Turner JR, Buschmann MM, Romero-Calvo I, Sailer A and Shen L: The role of molecular remodeling in differential regulation of tight junction permeability. Semin Cell Dev Biol. 36:204–212. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Fujita H, Chiba H, Yokozaki H, Sakai N, Sugimoto K, Wada T, Kojima T, Yamashita T and Sawada N: Differential expression and subcellular localization of claudin-7, −8, −12, −13, and −15 along the mouse intestine. J Histochem Cytochem. 54:933–944. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Holmes JL, Van Itallie CM, Rasmussen JE and Anderson JM: Claudin profiling in the mouse during postnatal intestinal development and along the gastrointestinal tract reveals complex expression patterns. Gene Expr Patterns. 6:581–588. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Inai T, Sengoku A, Guan X, Hirose E, Iida H and Shibata Y: Heterogeneity in expression and subcellular localization of tight junction proteins, claudin-10 and −15, examined by RT-PCR and immunofluorescence microscopy. Arch Histol Cytol. 68:349–360. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Faderl M, Noti M, Corazza N and Mueller C: Keeping bugs in check: The mucus layer as a critical component in maintaining intestinal homeostasis. IUBMB Life. 67:275–285. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Bailey MT, Dowd SE, Parry NM, Galley JD, Schauer DB and Lyte M: Stressor exposure disrupts commensal microbial populations in the intestines and leads to increased colonization by Citrobacter rodentium. Infect Immun. 78:1509–1519. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Cameron HL and Perdue MH: Stress impairs murine intestinal barrier function: Improvement by glucagon-like peptide-2. J Pharmacol Exp Ther. 314:214–220. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Reber SO, Peters S, Slattery DA, Hofmann C, Schölmerich J, Neumann ID and Obermeier F: Mucosal immunosuppression and epithelial barrier defects are key events in murine psychosocial stress-induced colitis. Brain Behav Immun. 25:1153–1161. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Söderholm JD, Yang PC, Ceponis P, Vohra A, Riddell R, Sherman PM and Perdue MH: Chronic stress induces mast cell-dependent bacterial adherence and initiates mucosal inflammation in rat intestine. Gastroenterology. 123:1099–1108. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Santos J, Yang PC, Söderholm JD, Benjamin M and Perdue MH: Role of mast cells in chronic stress induced colonic epithelial barrier dysfunction in the rat. Gut. 48:630–636. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Santos J, Yates D, Guilarte M, Vicario M, Alonso C and Perdue MH: Stress neuropeptides evoke epithelial responses via mast cell activation in the rat colon. Psychoneuroendocrinology. 33:1248–1256. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Vicario M, Guilarte M, Alonso C, Yang P, Martínez C, Ramos L, Lobo B, González A, Guilà M, Pigrau M, et al: Chronological assessment of mast cell-mediated gut dysfunction and mucosal inflammation in a rat model of chronic psychosocial stress. Brain Behav Immun. 24:1166–1175. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Vicario M, Alonso C, Guilarte M, Serra J, Martínez C, González-Castro AM, Lobo B, Antolín M, Andreu AL, García-Arumí E, et al: Chronic psychosocial stress induces reversible mitochondrial damage and corticotropin-releasing factor receptor type-1 upregulation in the rat intestine and IBS-like gut dysfunction. Psychoneuroendocrinology. 37:65–77. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Zheng G, Wu SP, Hu Y, Smith DE, Wiley JW and Hong S: Corticosterone mediates stress-related increased intestinal permeability in a region-specific manner. Neurogastroenterol Motil. 25:e127–e139. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Hattay P, Prusator DK, Tran L and Greenwood-Van Meerveld B: Psychological stress-induced colonic barrier dysfunction: Role of immune-mediated mechanisms. Neurogastroenterol Motil. 29:2017. View Article : Google Scholar : PubMed/NCBI

18 

Lauffer A, Vanuytsel T, Vanormelingen C, Vanheel H, Salim Rasoel S, Tóth J, Tack J, Fornari F and Farré R: Subacute stress and chronic stress interact to decrease intestinal barrier function in rats. Stress. 19:225–234. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Nébot-Vivinus M, Harkat C, Bzioueche H, Cartier C, Plichon-Dainese R, Moussa L, Eutamene H, Pishvaie D, Holowacz S, Seyrig C, et al: Multispecies probiotic protects gut barrier function in experimental models. World J Gastroenterol. 20:6832–6843. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Official Mexican Standard NOM-062-ZOO-1999, . Technical specifications for the production, care and use of laboratory animals. Secretary of agriculture, livestock, rural development, fisheries and Food (SAGARPA). Official Gazette, Mexican Federal Government. June 18–2001.

21 

Bhatia N, Maiti PP, Choudhary A, Tuli A, Masih D, Khan U, Ara T and Jaggi AS: Animal models in the study of stress: A review. NSHM J Pharm Healthcare Manage. 2:42–50. 2011.

22 

Uni Z, Smirnov A and Sklan D: Pre- and posthatch development of goblet cells in the broiler small intestine: Effect of delayed access to feed. Poult Sci. 82:320–327. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Logsdon LK and Mecsas J: A non-invasive quantitative assay to measure murine intestinal inflammation using the neutrophil marker lactoferrin. J Immunol Methods. 313:183–190. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Xiao Y and Isaacs SN: Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)-not all BSAs are alike. J Immunol Methods. 384:148–151. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Rozen S and Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 132:365–386. 2000.PubMed/NCBI

27 

Kyoko OO, Kono H, Ishimaru K, Miyake K, Kubota T, Ogawa H, Okumura K, Shibata S and Nakao A: Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: Implications in intestinal permeability and susceptibility to colitis. PLoS One. 9:e980162014. View Article : Google Scholar : PubMed/NCBI

28 

Liévin-L Moal V and Servin AL: The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: Mucins, antimicrobial peptides, and microbiota. Clin Microbiol Rev. 19:315–337. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Siddiqui I, Majid H and Abid S: Update on clinical and research application of fecal biomarkers for gastrointestinal diseases. World J Gastrointest Pharmacol Ther. 8:39–46. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Sandrini SM, Shergill R, Woodward J, Muralikuttan R, Haigh RD, Lyte M and Freestone PP: Elucidation of the mechanism by which catecholamine stress hormones liberate iron from the innate immune defense proteins transferrin and lactoferrin. J Bacteriol. 192:587–594. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Al-Sadi R, Khatib K, Guo S, Ye D, Youssef M and Ma T: Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier. Am J Physiol Gastrointest Liver Physiol. 300:G1054–G1064. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Ponferrada A, Caso JR, Alou L, Colón A, Sevillano D, Moro MA, Lizasoain I, Menchén P, Gómez-Lus ML, Lorenzo P, et al: The role of PPARgamma on restoration of colonic homeostasis after experimental stress-induced inflammation and dysfunction. Gastroenterology. 132:1791–1803. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Iraha A, Chinen H, Hokama A, Yonashiro T, Kinjo T, Kishimoto K, Nakamoto M, Hirata T, Kinjo N, Higa F, et al: Fucoidan enhances intestinal barrier function by upregulating the expression of claudin-1. World J Gastroenterol. 19:5500–5507. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Nevado R, Forcén R, Layunta E, Murillo MD and Grasa L: Neomycin and bacitracin reduce the intestinal permeability in mice and increase the expression of some tight-junction proteins. Rev Esp Enferm Dig. 107:672–676. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Koh SJ, Kim JW, Kim BG, Lee KL and Kim JS: Restraint stress induces and exacerbates intestinal inflammation in interleukin-10 deficient mice. World J Gastroenterol. 21:8580–8587. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Kim JJ and Khan WI: Goblet cells and mucins: Role in innate defense in enteric infections. Pathogens. 2:55–70. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Bourke CH, Harrell CS and Neigh GN: Stress-induced sex differences: Adaptations mediated by the glucocorticoid receptor. Horm Behav. 62:210–218. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Grishina I, Fenton A and Sankaran-Walters S: Gender differences, aging and hormonal status in mucosal injury and repair. Aging Dis. 5:160–169. 2014.PubMed/NCBI

39 

Andrews C, McLean MH and Durum SK: Cytokine tuning of intestinal epithelial function. Front Immunol. 9:12702018. View Article : Google Scholar : PubMed/NCBI

40 

Cakir B, Bozkurt A, Ercan F and Yeğen BC: The anti-inflammatory effect of leptin on experimental colitis: Involvement of endogenous glucocorticoids. Peptides. 25:95–104. 2004. View Article : Google Scholar : PubMed/NCBI

41 

Rijnierse A, Koster AS, Nijkamp FP and Kraneveld AD: TNF-alpha is crucial for the development of mast cell-dependent colitis in mice. Am J Physiol Gastrointest Liver Physiol. 291:G969–G976. 2006. View Article : Google Scholar : PubMed/NCBI

42 

Camilleri M, Sellin JH and Barrett KE: Pathophysiology, evaluation, and management of chronic watery diarrhea. Gastroenterology. 152:515–532.e2. 2017. View Article : Google Scholar : PubMed/NCBI

43 

Lapointe TK and Buret AG: Interleukin-18 facilitates neutrophil transmigration via myosin light chain kinase-dependent disruption of occludin, without altering epithelial permeability. Am J Physiol Gastrointest Liver Physiol. 302:G343–G351. 2012. View Article : Google Scholar : PubMed/NCBI

44 

Ananthakrishnan AN: Environmental triggers for inflammatory bowel disease. Curr Gastroenterol Rep. 15:3022013. View Article : Google Scholar : PubMed/NCBI

45 

Konturek PC, Brzozowski T and Konturek SJ: Stress and the gut: Pathophysiology, clinical consequences, diagnostic approach and treatment options. J Physiol Pharmacol. 62:591–599. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Machorro‑Rojas N, Sainz‑Espuñes T, Godínez‑Victoria M, Castañeda‑Sánchez JI, Campos‑Rodríguez R, Pacheco‑Yepez J and Drago‑Serrano ME: Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. Mol Med Rep 20: 2083-2090, 2019.
APA
Machorro‑Rojas, N., Sainz‑Espuñes, T., Godínez‑Victoria, M., Castañeda‑Sánchez, J.I., Campos‑Rodríguez, R., Pacheco‑Yepez, J., & Drago‑Serrano, M.E. (2019). Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. Molecular Medicine Reports, 20, 2083-2090. https://doi.org/10.3892/mmr.2019.10437
MLA
Machorro‑Rojas, N., Sainz‑Espuñes, T., Godínez‑Victoria, M., Castañeda‑Sánchez, J. I., Campos‑Rodríguez, R., Pacheco‑Yepez, J., Drago‑Serrano, M. E."Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice". Molecular Medicine Reports 20.3 (2019): 2083-2090.
Chicago
Machorro‑Rojas, N., Sainz‑Espuñes, T., Godínez‑Victoria, M., Castañeda‑Sánchez, J. I., Campos‑Rodríguez, R., Pacheco‑Yepez, J., Drago‑Serrano, M. E."Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice". Molecular Medicine Reports 20, no. 3 (2019): 2083-2090. https://doi.org/10.3892/mmr.2019.10437
Copy and paste a formatted citation
x
Spandidos Publications style
Machorro‑Rojas N, Sainz‑Espuñes T, Godínez‑Victoria M, Castañeda‑Sánchez JI, Campos‑Rodríguez R, Pacheco‑Yepez J and Drago‑Serrano ME: Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. Mol Med Rep 20: 2083-2090, 2019.
APA
Machorro‑Rojas, N., Sainz‑Espuñes, T., Godínez‑Victoria, M., Castañeda‑Sánchez, J.I., Campos‑Rodríguez, R., Pacheco‑Yepez, J., & Drago‑Serrano, M.E. (2019). Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. Molecular Medicine Reports, 20, 2083-2090. https://doi.org/10.3892/mmr.2019.10437
MLA
Machorro‑Rojas, N., Sainz‑Espuñes, T., Godínez‑Victoria, M., Castañeda‑Sánchez, J. I., Campos‑Rodríguez, R., Pacheco‑Yepez, J., Drago‑Serrano, M. E."Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice". Molecular Medicine Reports 20.3 (2019): 2083-2090.
Chicago
Machorro‑Rojas, N., Sainz‑Espuñes, T., Godínez‑Victoria, M., Castañeda‑Sánchez, J. I., Campos‑Rodríguez, R., Pacheco‑Yepez, J., Drago‑Serrano, M. E."Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice". Molecular Medicine Reports 20, no. 3 (2019): 2083-2090. https://doi.org/10.3892/mmr.2019.10437
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