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Article

Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells

  • Authors:
    • Jiajun Chen
    • Yue Zhou
    • Xiangyu Zhou
    • Jia Li
    • Feng Liu
    • Qingwei Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Biotherapeutics, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, P.R. China, Department of Hepatobiliary Surgery, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Nephrology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Biomedical Engineering Unit, Institute of Frontier Medical Sciences of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 2389-2395
    |
    Published online on: July 3, 2019
       https://doi.org/10.3892/mmr.2019.10459
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Abstract

The current study investigated the effect of plasmid small interfering RNA (psiRNA)‑mediated silencing of signal transducer and activator of transcription‑3 (STAT3) on the invasion, apoptosis, and expression levels of cyclin D1, caspase‑3 and B‑cell lymphoma‑2 (Bcl‑2) in C6 glioma cells. Cell invasion was determined using a Transwell assay, while the apoptosis rate and cell cycle distribution of cells were assessed using Annexin V‑FITC/PI double staining. The expression levels of cyclin D1, caspase‑3 and Bcl‑2 proteins were measured by western blotting. Transfection with psiRNA‑STAT3 was observed to significantly decrease the number of transmembrane cells compared with the control groups (P<0.05). In addition, the proportion of cells at G0/G1 phase was increased in the psiRNA‑STAT3 group compared with the controls. Western blotting indicated that psiRNA‑STAT3 decreased the expression of cyclin D1, caspase‑3 and Bcl‑2 proteins. Taken together, psiRNA‑STAT3 inhibited the migration and invasive abilities, and induced the apoptosis of C6 glioma cells, possibly through regulation of the expression of cyclin D1, caspase‑3 and Bcl‑2 proteins.
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1 

Roci E, Cakani B, Brace G, Bushati T, Rroji A, Petrela M and Kaloshi G: Platinum-based chemotherapy in recurrent high-grade glioma patients: Retrospective study. Med Arch. 68:140–143. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Feng B, Hu P, Lu SJ, Chen JB and Ge RL: Increased argonaute 2 expression in gliomas and its association with tumor progression and poor prognosis. Asian Pac J Cancer Prev. 15:4079–4083. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Davis ME and Stoiber AM: Glioblastoma multiforme: Enhancing survival and quality of life. Clin J Oncol Nurs. 15:291–297. 2011. View Article : Google Scholar : PubMed/NCBI

4 

McNamara MG and Mason WP: Antiangiogenic therapies in glioblastoma multiforme. Expert Rev Anticancer Ther. 12:643–654. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Vaupel P: Hypoxia and aggressive tumor phenotype: Implications for therapy and prognosis. Oncologist. 13:21–26. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Mazure NM and Pouysségur J: Hypoxia-induced autophagy: Cell death or cell survival? Curr Opin Cell Biol. 22:177–180. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Sayegh ET, Kaur G, Bloch O and Parsa AT: Systematic review of protein biomarkers of invasive behavior in glioblastoma. Mol Neurobiol. 49:1212–1244. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Zhou M, Wang H, Zhou K, Luo X, Pan X, Shi B, Jiang H, Zhang J, Li K, Wang HM, et al: A novel EGFR isoform confers increased invasiveness to cancer cells. Cancer Res. 73:7056–7067. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Coussens LM, Fingleton B and Matrisian LM: Matrix metalloproteinase inhibitors and cancer: Trials and tribulations. Science. 295:2387–2392. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Friedl P and Wolf K: Plasticity of cell migration: A multiscale tuning model. J Cell Biol. 188:11–19. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Hong SS, Lee H and Kim KW: HIF-1alpha: A valid therapeutic target for tumor therapy. Cancer Res Treat. 36:343–353. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Morioka Y, Monypenny J, Matsuzaki T, Shi S, Alexander DB, Kitayama H and Noda M: The membrane-anchored metalloproteinase regulator RECK stabilizes focal adhesions and anterior-posterior polarity in fibroblasts. Oncogene. 28:1454–1464. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Doucette TA, Kong LY, Yang Y, Ferguson SD, Yang J, Wei J, Qiao W, Fuller GN, Bhat KP, Aldape K, et al: Signal transducer and activator of transcription 3 promotes angiogenesis and drives malignant progression in glioma. Neuro Oncol. 14:1136–1145. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Zhou XY, Tian L, Zhou Y, Dai L, Liu S and Zhou QW: The effect of Lipofectamine 2000 plasmid on glioma C6 cells. Zhongguo Laonianxue Zazhi. 36:3404–3406. 2016.

16 

Yuan J, Zhang F and Niu R: Multiple regulation pathways and pivotal biological functions of STAT3 in cancer. Sci Rep. 5:176632015. View Article : Google Scholar : PubMed/NCBI

17 

Yu H, Lee H, Herrmann A, Buettner R and Jove R: Revisiting STAT3 signalling in cancer: New and unexpected biological functions. Nat Rev Cancer. 14:736–746. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Geiger JL, Grandis JR and Bauman JE: The STAT3 pathway as a therapeutic target in head and neck cancer: Barriers and innovations. Oral Oncol. 56:84–92. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Tanaka S, Suto A, Iwamoto T, Kashiwakuma D, Kagami S, Suzuki K, Takatori H, Tamachi T, Hirose K, Onodera A, et al: Sox5 and c-Maf cooperatively induce Th17 cell differentiation via RORγt induction as downstream targets of Stat3. J Exp Med. 211:1857–1874. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Yang YC, Huang YT, Hsieh CW, Yang PM and Wung BS: Carbon monoxide induces heme oxygenase-1 to modulate STAT3 activation in endothelial cells via S-glutathionylation. PLoS One. 9:e1006772014. View Article : Google Scholar : PubMed/NCBI

21 

Doucette TA, Kong LY, Yang Y, Ferguson SD, Yang J, Wei J, Qiao W, Fuller GN, Bhat KP, Aldape K, Priebe W, Bogler O, Heimberger AB and Rao G.: Signal transducer and activator of transcription-3 promotes angiogenesis and drives malignant progression in glioma. Neuro Oncol. 14(9): 1136–45. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Inaba T, Matsushime H, Valentine M, Roussel MF, Sherr CJ and Look AT: Genomic organization, chromosomal localization, and independent expression of human cyclin D genes. Genomics. 13:565–574. 1992. View Article : Google Scholar : PubMed/NCBI

23 

Vansaun MN: Molecular pathways: Adiponectin and leptin signaling in cancer. Clin Cancer Res. 19:1926–1932. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Fox MM, Phoenix KN, Kopsiaftis SG and Claffey KP: AMP-activated protein kinase α 2 isoform suppression in primary breast cancer alters ampk growth control and apoptotic signaling. Genes Cancer. 4:3–14. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Zhuang Y, Chan DK, Haugrud AB and Miskimins WK: Mechanisms by which low glucose enhances the cytotoxicity of metformin to cancer cells both in vitro and in vivo. PLoS One. 9:e1084442014. View Article : Google Scholar : PubMed/NCBI

26 

Yihua Chen YH, Zhang HJ and Nan FJ: Progress in the research of Caspases inhibitor. Shengwu Jishu. 18:248–253. 2006.

27 

Sikdar S, Mukherjee A, Ghosh S and Khuda-Bukhsh AR: Condurango glycoside-rich components stimulate DNA damage-induced cell cycle arrest and ROS-mediated caspase-3 dependent apoptosis through inhibition of cell-proliferation in lung cancer, in vitro and in vivo. Environ Toxicol Pharmacol. 37:300–314. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Nahta R and Esteva FJ: Bcl-2 antisense oligonucleotides: A potential novel strategy for the treatment of breast cancer. Semin Oncol. 30:143–149. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Zhou Y, Zhou X, Li J, Liu F and Zhou Q: Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells. Mol Med Rep 20: 2389-2395, 2019.
APA
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., & Zhou, Q. (2019). Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells. Molecular Medicine Reports, 20, 2389-2395. https://doi.org/10.3892/mmr.2019.10459
MLA
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., Zhou, Q."Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells". Molecular Medicine Reports 20.3 (2019): 2389-2395.
Chicago
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., Zhou, Q."Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells". Molecular Medicine Reports 20, no. 3 (2019): 2389-2395. https://doi.org/10.3892/mmr.2019.10459
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Zhou Y, Zhou X, Li J, Liu F and Zhou Q: Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells. Mol Med Rep 20: 2389-2395, 2019.
APA
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., & Zhou, Q. (2019). Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells. Molecular Medicine Reports, 20, 2389-2395. https://doi.org/10.3892/mmr.2019.10459
MLA
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., Zhou, Q."Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells". Molecular Medicine Reports 20.3 (2019): 2389-2395.
Chicago
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., Zhou, Q."Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells". Molecular Medicine Reports 20, no. 3 (2019): 2389-2395. https://doi.org/10.3892/mmr.2019.10459
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