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Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40

  • Authors:
    • Ming Yu
    • Demei Zang
    • Yuhao Xu
    • Jie Meng
    • Shengnan Qian
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2135-2142
    |
    Published online on: July 9, 2019
       https://doi.org/10.3892/mmr.2019.10483
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Abstract

Advanced glycation end products (AGEs) are important pathogenic substances involved in diabetes mellitus (DM) and its complications. AGEs also serve important roles in promoting the development of Alzheimer's disease (AD). Macrophage migration inhibitory factor (MIF), an inflammatory stimulant and a pathogenic factor involved in DM, was previously reported to be present at increased levels in the cerebrospinal fluid of patients with AD and mild cognitive impairment compared with age‑matched healthy controls. By investigating the association between AGEs and MIF, and the effects of neuroinflammation on AD, the present study aimed to increase understanding of the specific molecular mechanisms involved in the pathogenesis of DM and AD, and the connection between these diseases. PC12 cells were cultured in vitro; the levels of MIF mRNA and protein were determined using reverse transcription‑quantitative (RT‑q)PCR and western blot analyses. The optimal concentrations of AGEs and amyloid β 1‑40 (Aβ1‑40) were also determined in the cell model of AD using Cell Counting Kit‑8 and MTT assays. Cell numbers and morphological changes were observed following the treatment of Aβ1‑40‑stimulated PC12 cells with AGEs and the MIF inhibitor (S,R)‑3‑(4‑hydroxyphenyl)‑4,5‑dihydro‑5‑isoxazole acetic acid methyl ester (ISO‑1). The mRNA expression levels of interleukin (IL)‑1β, IL‑6, tumor necrosis factor‑α (TNF‑α) and MIF were determined via RT‑qPCR analysis. The results showed that the levels of MIF mRNA and protein were significantly increased in cells treated with AGEs compared with the control group. In the AD model group, the inhibition of PC12 cell growth was significantly increased, and the mRNA expression levels of IL‑1β, IL‑6, TNF‑α and MIF were also increased. Compared with treatment with AGEs alone, the combination of AGEs treatment with ISO‑1 significantly improved the survival rate and resulted in the reduced expression of inflammatory mediators in the AD cell model. Thus, ISO‑1 reduced AGEs‑mediated damage in the AD cell model. This may be a consequence of AGEs‑mediated MIF expression promoting neuritis in the AD cell model, whereas ISO‑1 decreased the expression of neuroinflammatory mediators.
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View References

1 

Alzheimer's Association: 2017 Alzheimer's disease facts and figures. Alzheimer's Dementia. 13:325–373. 2017. View Article : Google Scholar

2 

Mushtaq G, Khan JA, Kumosani TA and Kamal MA: Alzheimer's disease and type 2 diabetes via chronic inflammatory mechanisms. Saudi J Biol Sci. 22:4–13. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Prince M, Bryce R, Albanese E, Wimo A, Ribeiro W and Ferri CP: The global prevalence of dementia: A systematic review and meta analysis. Alzheimers Dement. 9:63–75. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Rawlings AM, Sharrett AR, Schneider AL, Coresh J, Albert M, Couper D, Griswold M, Gottesman RF, Wagenknecht LE, Windham BG and Selvin E: Diabetes in midlife and cognitive change over 20 years: A cohort study. Ann Intern Med. 161:785–793. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Ohara T, Doi Y, Ninomiya T, Hirakawa Y, Hata J, Iwaki T, Kanba S and Kiyohara Y: Glucose tolerance status and risk of dementia in the community: The hisayama study. Neurology. 77:1126–1134. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Kandimalla R, Thirumala V and Reddy PH: Is Alzheimer's disease a type 3 diabetes? A critical appraisal. Biochim Biophys Acta Mol Basis Dis. 1863:1078–1089. 2017. View Article : Google Scholar : PubMed/NCBI

7 

de la Monte SM and Wands JR: Alzheimer's disease is type 3 diabetes-evidence reviewed. J Diabetes Sci Technol. 2:1101–1113. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Steen E, Terry BM, Rivera EJ, Cannon JL, Neely TR, Tavares R, Xu XJ, Wands JR and de la Monte SM: Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer's disease-is this type 3 diabetes? J Alzheimers Dis. 7:63–80. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Rivera EJ, Goldin A, Fulmer N, Tavares R, Wands JR and de la Monte SM: Insulin and insulin-like growth factor expression and function deteriorate with progressionof Alzheimer's disease: Link to brain reductions in acet ylcholine. J Alzheimers Dis. 8:247–268. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Goh SY and Cooper ME: Clinical review: The role of advanced glycation end products in progression and complications of diabetes. J Clin Endocrinol Metab. 93:1143–1152. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Tanaka K, Yamaguchi T, Kanazawa I and Sugimoto T: Effects of high glucose and advanced glycation end products on the expressions of sclerostin and RANKL as well as apoptosis in osteocyte-like MLO-Y4-A2 cells. Biochem Biophys Res Commun. 461:193–199. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Chang CC, Chen CY, Chang GD, Chen TH, Chen WL, Wen HC, Huang C and Chang CH: Hyperglycemia and advanced glycation end products (AGEs) suppress the differentiation of 3T3-L1 preadipocytes. Oncotarget. 8:55039–55050. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Lin JA, Wu CH, Lu CC, Hsia SM and Yen GC: Glycative stress from advanced glycation end products (AGEs) and dicarbonyls: An emerging biological factor in cancer onset and progression. Mol Nutr Food Res. 60:1850–1864. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Sato T, Shimogaito N, Wu X, Kikuchi S, Yamagishi S and Takeuchi M: Toxic advanced glycation end products (TAGE) theory in Alzheimer's disease. Am J A1zheimers Dis Other Demen 2l. 197–208. 2006. View Article : Google Scholar

15 

Vitek MP, Bhattacharya K, Glendening JM, Stopa E, Vlassara H, Bucala R, Manogue K and Cerami A: Advanced glycation end products contribute to amyloidosis in alzheimer disease. Proc Natl Acad Sci USA. 91:4766–4770. 1994. View Article : Google Scholar : PubMed/NCBI

16 

Lue H, Kleemann R, Calandra T, Roger T and Bernhagen J: Macrophage migration inhibitory factor (MIF): Mechanisms of action and role in disease. Microbes Infection. 4:449–460. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Grieb G, Merk M, Bernhagen J and Bucala R: Macrophage migration inhibitory factor(MIF): A promising biomarker. Drug News Perspect. 23:257–264. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Abed Y, Dabideen D, Aljabari B, Valster A, Messmer D, Ochani M, Tanovic M, Ochani K, Bacher M, Nicoletti F, et al: ISO-1 binding to the tautomerase activesite of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J Biol Chem. 280:36541–36544. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Vujicic M, Senerovic L, Nikolic I, Saksida T, Stosic-Grujicic S and Stojanovic I: The critical role of macrophage migration inhibitory factor in insulin activity. Cytokine. 69:39–46. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Bacher M, Deuster O, Aljabari B, Egensperger R, Neff F, Jessen F, Popp J, Noelker C, Reese JP, Al-Abed Y and Dode R: The role of macrophage migration inhibitory factor in Alzheimer's disease. Mol Med. 16:116–121. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Oyama R, Yamamoto H and Titani K: Glutamine synthetase, hemoglobin alpha-chain, and macrophage migration inhibitory factor binding to amyloid beta-protein: Their identification in rat brain by a novel affinity chromatography and in Alzheimer's disease brain by immunoprecipitation. Biochim Biophys Acta. 1479:91–102. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Li SQ, Yu Y, Han JZ, Wang D, Liu J, Qian F, Fan GH, Bucala R and Ye RD: Deficiency of macrophage migration inhibitory factor attenuates tau hyperphosphorylation in mouse models of Alzheimer's disease. J Neuroinflammation. 12:1–11. 2015. View Article : Google Scholar

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Luo XD, Xu B, Zhou J and Tian GP: Protective effect of secretory factors of bone marrow mesenchymal stem cells on beta-amyloid 1–40 induced apoptosis in PC12 cells. J Clin Rehabilitat Tissue Eng Res. 13:7937–7941. 2009.

25 

Kanehisa M, Furumichi M, Tanabe M, Sato Y and Morishima K: KEGG: New perspectives on genomes, pathways, diseases and drugs. Nucleic Acids Res. 45:D353–D361. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Benigni F, Atsunmi T, Calandra T, Metz C, Echtenacher B, Peng T and Bucala R: The proinflammatory mediator macrophage migration inhibitory factor induces glucose cataboilsm in muscle. J Clin Invest. 106:1291–1300. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Blennow K, de Leon MJ and Zetterberg H: Alzheimer's disease. Lancet. 368:387–403. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Nagae T, Araki K, Shimoda Y, Sue LI, Beach TG and Konishi Y: Cytokines and cytokine receptors involved in the pathogenesis of Alzheimer's disease. J Clin Cell Immunol. 7:4412016. View Article : Google Scholar : PubMed/NCBI

29 

Heneka MT, Carson MJ, EI Khoury J, Landreth GE, Brosseron F, Feinstein DL, Jacobs AH, Wyss-Coray T, Vitorica J, Ransohoff RM, et al: Neuroinflammation in Alzheimer's disease. Lancet Neurol. 14:388–405. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Azizi G and Mirshafiey A: The potential role of proinflammatory and antiinflammatory cytokines in Alzheimer disease pathogenesis. Immunopharmacol Immunotoxicol. 34:881–895. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Azizi G, Navabi SS, Al-Shukaili A, Seyedzadeh MH, Yazdani R and Mirshafiey A: The role of inflammatory mediators in the pathogenesis of Alzheimer's disease. Sultan Qaboos Univ Med J. 15:e305–e316. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Azizi G, Khannazer N and Mirshafiey A: The potential role of chemokines in Alzheimer's disease pathogenesis. Am J Alzheimers Dis Other Demen. 29:415–425. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Zhang Y, Gu R, Jia J, Hou T, Zheng LT and Zhen X: Inhibition of macrophage migration inhibitory factor (MIF) tautomerase activity suppresses microglia-mediated inflammatory responses. Clin Exp Pharmacol Physiol. 43:1134–1144. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu M, Zang D, Xu Y, Meng J and Qian S: Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40. Mol Med Rep 20: 2135-2142, 2019.
APA
Yu, M., Zang, D., Xu, Y., Meng, J., & Qian, S. (2019). Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40. Molecular Medicine Reports, 20, 2135-2142. https://doi.org/10.3892/mmr.2019.10483
MLA
Yu, M., Zang, D., Xu, Y., Meng, J., Qian, S."Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40". Molecular Medicine Reports 20.3 (2019): 2135-2142.
Chicago
Yu, M., Zang, D., Xu, Y., Meng, J., Qian, S."Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40". Molecular Medicine Reports 20, no. 3 (2019): 2135-2142. https://doi.org/10.3892/mmr.2019.10483
Copy and paste a formatted citation
x
Spandidos Publications style
Yu M, Zang D, Xu Y, Meng J and Qian S: Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40. Mol Med Rep 20: 2135-2142, 2019.
APA
Yu, M., Zang, D., Xu, Y., Meng, J., & Qian, S. (2019). Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40. Molecular Medicine Reports, 20, 2135-2142. https://doi.org/10.3892/mmr.2019.10483
MLA
Yu, M., Zang, D., Xu, Y., Meng, J., Qian, S."Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40". Molecular Medicine Reports 20.3 (2019): 2135-2142.
Chicago
Yu, M., Zang, D., Xu, Y., Meng, J., Qian, S."Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40". Molecular Medicine Reports 20, no. 3 (2019): 2135-2142. https://doi.org/10.3892/mmr.2019.10483
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