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Article

Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development

  • Authors:
    • Xiaoqing Wang
    • Xiaoxiao Wang
    • Yingfang Zhou
    • Chao Peng
    • Huayun Chen
    • Ye Lu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, P.R. China, Department of Obstetrics and Gynecology, Beijing Tsinghua Changgeng Hospital, Beijing 102218, P.R. China
  • Pages: 2859-2866
    |
    Published online on: July 15, 2019
       https://doi.org/10.3892/mmr.2019.10501
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Abstract

The present study aimed to investigate the effects of Mitofusin2 (Mfn2) on the proliferation of human uterosacral ligament fibroblasts and on the expression of procollagen. We also aimed to identify the possible signal transduction pathway involved in the development of pelvic organ prolapse (POP). For this purpose, uterosacral ligaments were harvested from POP and non‑pelvic organ prolapse (NPOP) patients for fibroblast culture. Cellular proliferation and the cell cycle were assessed following transduction with lentiviral vectors for the overexpression and suppression of Mfn2. The expression levels of the proteins Mfn2, procollagens, phosphoprotein 21 wild‑type p53 activating fragment (p21Waf1), cyclin‑dependent kinase 2 (CDK2), extracellular signal‑regulated kinase1/2 (ERK1/2) and rapidly accelerated fibrosarcoma‑1 (Raf‑1) were examined. Overexpression of Mfn2 resulted in the decreased proliferation of cells and the induction of G0/G1 phase arrest. Concomitantly, the relative expression levels of procollagen proteins, CDK2 and the phosphorylation levels of ERK1/2 and Raf‑1 proteins were notably decreased, while the levels of the p21waf1 protein were increased in the Mfn2 overexpressing group. Opposing results were reported cells following Mfn2 silencing via RNA interference. The results of the present study indicated that the cell cycle of the fibroblasts, their cellular proliferation and the levels of the procollagen proteins could be inhibited via the Ras‑Raf‑ERK axis as a result of the increased levels of Mfn2 during the development of POP.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Wang X, Zhou Y, Peng C, Chen H and Lu Y: Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development. Mol Med Rep 20: 2859-2866, 2019.
APA
Wang, X., Wang, X., Zhou, Y., Peng, C., Chen, H., & Lu, Y. (2019). Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development. Molecular Medicine Reports, 20, 2859-2866. https://doi.org/10.3892/mmr.2019.10501
MLA
Wang, X., Wang, X., Zhou, Y., Peng, C., Chen, H., Lu, Y."Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development". Molecular Medicine Reports 20.3 (2019): 2859-2866.
Chicago
Wang, X., Wang, X., Zhou, Y., Peng, C., Chen, H., Lu, Y."Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development". Molecular Medicine Reports 20, no. 3 (2019): 2859-2866. https://doi.org/10.3892/mmr.2019.10501
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Wang X, Zhou Y, Peng C, Chen H and Lu Y: Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development. Mol Med Rep 20: 2859-2866, 2019.
APA
Wang, X., Wang, X., Zhou, Y., Peng, C., Chen, H., & Lu, Y. (2019). Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development. Molecular Medicine Reports, 20, 2859-2866. https://doi.org/10.3892/mmr.2019.10501
MLA
Wang, X., Wang, X., Zhou, Y., Peng, C., Chen, H., Lu, Y."Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development". Molecular Medicine Reports 20.3 (2019): 2859-2866.
Chicago
Wang, X., Wang, X., Zhou, Y., Peng, C., Chen, H., Lu, Y."Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development". Molecular Medicine Reports 20, no. 3 (2019): 2859-2866. https://doi.org/10.3892/mmr.2019.10501
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