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Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2

  • Authors:
    • Wen Xu
    • Quan Xu
    • Dinghua Kuang
    • Zhongjun Wang
    • Qiuliang Lu
    • Qing Lin
    • Hao Wu
    • Liru Chen
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Thoracic Surgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of General Surgery, People's Hospital of Shanggao County, Yichun, Jiangxi 336400, P.R. China, Department of General Surgery, Ji'an County People's Hospital, Ji'an, Jiangxi 343100, P.R. China, Department of Thoracic Surgery, People's Hospital of Yichun City, Yichun, Jiangxi 336000, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2591-2596
    |
    Published online on: July 23, 2019
       https://doi.org/10.3892/mmr.2019.10518
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Abstract

Long non‑coding RNA (lncRNA) SRA‑like non‑coding RNA (SLNCR1; also known as linc00673) is a recently identified oncogenic lncRNA. The role of SLNCR1 in non‑small cell lung cancer (NSCLC), a common malignancy, remains poorly understood. The present study aimed to investigate the involvement of long non‑coding RNA SLNCR1 in the pathogenesis of NSCLC. Reverse transcription‑quantitative PCR (RT‑qPCR) and ELISA were performed to measure the levels of lncRNA SLNCR1 and secretory phospholipase A2 (sPLA2) in lung biopsies, respectively. Correlations between expression levels of lncRNA SLNCR1 and sPLA2 were analyzed by Pearson's correlation analysis. Diagnostic value of lncRNA SLNCR1 for NSCLC was evaluated by receiver operating characteristic curve analysis. lncRNA SLNCR1 small interfering (si)RNA and sPLA2 overexpression vector were transfected into NSCLC cells. Cell migration, invasion and stemness were examined by Transwell migration assay, Matrigel invasion assay and flow cytometric assay, respectively. Following transfection, expression of lncRNA SLNCR1 and sPLA2 was detected by RT‑qPCR and western blotting, respectively. The results demonstrated that lncRNA SLNCR1 expression levels were upregulated in tumor tissues compared with adjacent healthy tissues of patients with NSCLC. Plasma lncRNA SLNCR1 and sPLA2 expression levels were upregulated in patients with NSCLC compared with healthy controls. Plasma lncRNA SLNCR1 and sPLA2 were positively correlated in patients with NSCLC, but not in healthy controls. lncRNA SLNCR1 siRNA silencing inhibited, whereas sPLA2 overexpression promoted cell migratory and invasive abilities and stemness. lncRNA SLNCR1 siRNA silencing led to downregulated sPLA2 expression, whereas sPLA2 overexpression did not significantly affect lncRNA SLNCR1 expression. sPLA2 overexpression attenuated the inhibitory effects of lncRNA SLNCR1 siRNA silencing on cell migration, invasion and stemness. In conclusion, lncRNA SLNCR1 may regulate cancer cell migration, invasion and stemness in NSCLC through interactions with sPLA2.
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1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Rahib L, Smith BD, Aizenberg R, Rosenzweig AB, Fleshman JM and Matrisian LM: Projecting cancer incidence and deaths to 2030: The unexpected burden of thyroid, liver, and pancreas cancers in the United States. Cancer Res. 74:2913–2921. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Guo Y, Zeng H, Zheng R, Li S, Barnett AG, Zhang S, Zou X, Huxley R, Chen W and Williams G: The association between lung cancer incidence and ambient air pollution in China: A spatiotemporal analysis. Environ Res. 144:60–65. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Zheng X, Schipper M, Kidwell K, Lin J, Reddy R, Ren Y, Chang A, Lv F, Orringer M and Spring Kong FM: Survival outcome after stereotactic body radiation therapy and surgery for stage I non-small cell lung cancer: A meta-analysis. Int J Radiat oncol Biol Phys. 90:603–611. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Sperduto PW, Yang TJ, Beal K, Pan H, Brown PD, Bangdiwala A, Shanley R, Yeh N, Gaspar LE, Braunstein S, et al: Estimating survival in patients with lung cancer and brain metastases: An update of the graded prognostic assessment for lung cancer using molecular markers (Lung-molGPA). JAMA Oncol. 3:827–831. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Schewe M, Franken PF, Sacchetti A, Schmitt M, Joosten R, Böttcher R, van Royen ME, Jeammet L, Payré C, Scott PM, et al: Secreted phospholipases A2 are intestinal stem cell niche factors with distinct roles in homeostasis, inflammation and cancer. Cell Stem Cell. 19:38–51. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Quach ND, Arnold RD and Cummings BS: Secretory phospholipase A2 enzymes as pharmacological targets for treatment of disease. Biochem Pharmacol. 90:338–348. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Pedada SR, Yarla NS, Tambade PJ, Dhananjaya BL, Bishayee A, Arunasree KM, Philip GH, Dharmapuri G, Aliev G, Putta S and Rangaiah G: Synthesis of new secretory phospholipase A2-inhibitory indole containing isoxazole derivatives as anti-inflammatory and anticancer agents. Eur J Med Chem. 112:289–297. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Rubio JM, Rodríguez JP, Gil-de-Gómez L, Guijas C, Balboa MA and Balsinde J: Group V secreted phospholipase A2 is upregulated by IL-4 in human macrophages and mediates phagocytosis via hydrolysis of ethanolamine phospholipids. J Immunol. 194:3327–3339. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Wu Y, Li Y, Shang M, Jian Y, Wang C, Bardeesi AS, Li Z, Chen T, Zhao L, Zhou L, et al: Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signalling. Parasit Vectors. 10:1472017. View Article : Google Scholar : PubMed/NCBI

12 

Lu W, Zhang H, Niu Y, Wu Y, Sun W, Li H, Kong J, Ding K, Shen HM, Wu H, et al: Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p. Mol Cancer. 16:1182017. View Article : Google Scholar : PubMed/NCBI

13 

Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon CH and Novina CD: The lncRNA SLNCR1 mediates melanoma invasion through a conserved SRA1-like region. Cell Rep. 15:2025–2037. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Hulstaert E, Brochez L, Volders PJ, Vandesompele J and Mestdagh P: Long non-coding RNAs in cutaneous melanoma: Clinical perspectives. Oncotarget. 8:43470–43480. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Lu S and Dong Z: overexpression of secretory phospholipase A2-IIa supports cancer stem cell phenotype via HER/ERBB-elicited signaling in lung and prostate cancer cells. Int J Oncol. 50:2113–2122. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Brglez V, Pucer A, Pungerčar J, Lambeau G and Petan T: Secreted phospholipases A2 are differentially expressed and epigenetically silenced in human breast cancer cells. Biochem Biophys Res Commun. 445:230–235. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Choi YA, Lim HK, Kim JR, Lee CH, Kim YJ, Kang SS and Baek SH: Group IB secretory phospholipase A2 promotes matrix metalloproteinase-2-mediated cell migration via the phosphatidylinositol-3 kinase and Akt pathway. J Biol Chem. 279:36579–36585. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Wu Y and Wu PY: CD133 as a marker for cancer stem cells: Progresses and concerns. Stem Cells Dev. 18:1127–1134. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu W, Xu Q, Kuang D, Wang Z, Lu Q, Lin Q, Wu H and Chen L: Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2. Mol Med Rep 20: 2591-2596, 2019.
APA
Xu, W., Xu, Q., Kuang, D., Wang, Z., Lu, Q., Lin, Q. ... Chen, L. (2019). Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2. Molecular Medicine Reports, 20, 2591-2596. https://doi.org/10.3892/mmr.2019.10518
MLA
Xu, W., Xu, Q., Kuang, D., Wang, Z., Lu, Q., Lin, Q., Wu, H., Chen, L."Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2". Molecular Medicine Reports 20.3 (2019): 2591-2596.
Chicago
Xu, W., Xu, Q., Kuang, D., Wang, Z., Lu, Q., Lin, Q., Wu, H., Chen, L."Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2". Molecular Medicine Reports 20, no. 3 (2019): 2591-2596. https://doi.org/10.3892/mmr.2019.10518
Copy and paste a formatted citation
x
Spandidos Publications style
Xu W, Xu Q, Kuang D, Wang Z, Lu Q, Lin Q, Wu H and Chen L: Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2. Mol Med Rep 20: 2591-2596, 2019.
APA
Xu, W., Xu, Q., Kuang, D., Wang, Z., Lu, Q., Lin, Q. ... Chen, L. (2019). Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2. Molecular Medicine Reports, 20, 2591-2596. https://doi.org/10.3892/mmr.2019.10518
MLA
Xu, W., Xu, Q., Kuang, D., Wang, Z., Lu, Q., Lin, Q., Wu, H., Chen, L."Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2". Molecular Medicine Reports 20.3 (2019): 2591-2596.
Chicago
Xu, W., Xu, Q., Kuang, D., Wang, Z., Lu, Q., Lin, Q., Wu, H., Chen, L."Long non‑coding RNA SLNCR1 regulates non‑small cell lung cancer migration, invasion and stemness through interactions with secretory phospholipase A2". Molecular Medicine Reports 20, no. 3 (2019): 2591-2596. https://doi.org/10.3892/mmr.2019.10518
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