Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2019 Volume 20 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2019 Volume 20 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells

  • Authors:
    • Bing Liu
    • Zheng‑Zhi Liu
    • Mei‑Ling Zhou
    • Jian‑Wei Lin
    • Xue‑Mei Chen
    • Zhu Li
    • Wen‑Bin Gao
    • Zhen‑Dong Yu
    • Tao Liu
  • View Affiliations / Copyright

    Affiliations: Department of Biotherapy and Oncology, Shenzhen Luohu People's Hospital, Shenzhen, Guangdong 518001, P.R. China, Department of Laboratory, Women and Children Health Institute of Futian, Shenzhen, Guangdong 518045, P.R. China, Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2823-2831
    |
    Published online on: July 25, 2019
       https://doi.org/10.3892/mmr.2019.10529
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In recent years, cellular immunotherapy has served an important role in the combined treatment of hepatocellular carcinoma. The possibility of specific cell therapies for the treatment of solid tumours has been further explored following the success of chimeric antigen receptor (CAR)‑T cell therapy in the treatment of haematological tumours. The present study aimed to evaluate the specificity and efficiency of c‑MET‑targeted CAR‑NK cell immunotherapy on human liver cancer in vitro. A CAR structure that targeted and recognised a c‑MET antigen was constructed. c‑MET‑CAR was transferred into primary NK cells using lentiviral infection. c‑MET‑positive HepG2 cells were used as an in vitro study model. The cytotoxicity assay results revealed that c‑MET‑CAR‑NK cells exhibited more specific cytotoxicity for HepG2 cells with high c‑MET expression compared with the lung cancer cell line H1299, which has low levels of c‑MET expression. The results of the present study demonstrated that c‑MET may be a specific and effective target for human liver cancer cell CAR‑NK immunotherapy. Based on these results, CAR‑NK cell‑based immunotherapy may provide a potential biotherapeutic approach for liver cancer treatment in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Yuan P, Chen TH, Chen ZW and Lin XQ: Calculation of life-time death probability due malignant tumors based on a sampling survey area in China. Asian Pac J Cancer Prev. 15:4307–4309. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Omata M, Cheng AL, Kokudo N, Kudo M, Lee JM, Jia J, Tateishi R, Han KH, Chawla YK, Shiina S, et al: Asia-Pacific clinical practice guidelines on the management of hepatocellular carcinoma: A 2017 update. Hepatol Int. 11:317–370. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Ma W, Wu L, Zhou F, Hong Z, Yuan Y and Liu Z: T cell-associated immunotherapy for hepatocellular carcinoma. Cell Physiol Biochem. 41:609–622. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Wang Z, Wu Z, Liu Y and Han W: New development in CAR-T cell therapy. J Hematol Oncol. 10:532017. View Article : Google Scholar : PubMed/NCBI

5 

DeFrancesco L: CAR-T cell therapy seeks strategies to harness cytokine storm. Nat Biotechnol. 32:6042014. View Article : Google Scholar : PubMed/NCBI

6 

Klingemann H: Are natural killer cells superior CAR drivers? Oncoimmunology. 3:e281472014. View Article : Google Scholar : PubMed/NCBI

7 

Glienke W, Esser R, Priesner C, Suerth JD, Schambach A, Wels WS, Grez M, Kloess S, Arseniev L and Koehl U: Advantages and applications of CAR-expressing natural killer cells. Front Pharmacol. 6:212015. View Article : Google Scholar : PubMed/NCBI

8 

Bordon Y: Tumour immunology: Natural killer cells spy greedy tumours. Nat Rev Immunol. 18:772018. View Article : Google Scholar : PubMed/NCBI

9 

Rosenberg EB, Herberman RB, Levine PH, Halterman RH, McCoy JL and Wunderlich JR: Lymphocyte cytotoxicity reactions to leukemia-associated antigens in identical twins. Int J Cancer. 9:648–658. 1972. View Article : Google Scholar : PubMed/NCBI

10 

Qian X, Wang X and Jin H: Cell transfer therapy for cancer: Past, present, and future. J Immunol Res. 2014:5259132014. View Article : Google Scholar : PubMed/NCBI

11 

Vivier E, Raulet DH, Moretta A, Caligiuri MA, Zitvogel L, Lanier LL, Yokoyama WM and Ugolini S: Innate or adaptive immunity? The example of natural killer cells. Science. 331:44–49. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Bradley M, Zeytun A, Rafi-Janajreh A, Nagarkatti PS and Nagarkatti M: Role of spontaneous and interleukin-2-induced natural killer cell activity in the cytotoxicity and rejection of Fas+ and Fas- tumor cells. Blood. 92:4248–4255. 1998.PubMed/NCBI

13 

Screpanti V, Wallin RP, Ljunggren HG and Grandien A: A central role for death receptor-mediated apoptosis in the rejection of tumors by NK cells. J Immunol. 167:2068–2073. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Kayagaki N, Yamaguchi N, Nakayama M, Takeda K, Akiba H, Tsutsui H, Okamura H, Nakanishi K, Okumura K and Yagita H: Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol. 163:1906–1913. 1999.PubMed/NCBI

15 

Brehm C, Huenecke S, Esser R, Kloess S, Quaiser A, Betz S, Zimmermann O, Soerensen J, Passweg JR, Klingebiel T, et al: Interleukin-2-stimulated natural killer cells are less susceptible to mycophenolate mofetil than non-activated NK cells: possible consequences for immunotherapy. Cancer Immunol Immunother. 63:821–833. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Campbell KS and Hasegawa J: Natural killer cell biology: An update and future directions. J Allergy Clin Immunol. 132:536–544. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Guillerey C, Huntington ND and Smyth MJ: Targeting natural killer cells in cancer immunotherapy. Nat Immunol. 17:1025–1036. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Rezvani K, Rouce R, Liu E and Shpall E: Engineering natural killer cells for cancer immunotherapy. Mol Ther. 25:1769–1781. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Mehta RS and Rezvani K: Chimeric antigen receptor expressing natural killer cells for the immunotherapy of cancer. Front Immunol. 9:2832018. View Article : Google Scholar : PubMed/NCBI

20 

Fasolo A, Sessa C, Gianni L and Broggini M: Seminars in clinical pharmacology: An introduction to MET inhibitors for the medical oncologist. Ann Oncol. 24:14–20. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Furge KA, Zhang YW and Vande Woude GF: Met receptor tyrosine kinase: Enhanced signaling through adapter proteins. Oncogene. 19:5582–5589. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Ma PC, Maulik G, Christensen J and Salgia R: c-Met: Structure, functions and potential for therapeutic inhibition. Cancer Metastasis Rev. 22:309–325. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Zhang YW, Su Y, Volpert OV and Vande Woude GF: Hepatocyte growth factor/scatter factor mediates angiogenesis through positive VEGF and negative thrombospondin 1 regulation. Proc Natl Acad Sci USA. 100:12718–12723. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Zhuang PH, Xu L, Gao L, Lu W, Ruan LT and Yang J: Correlations of microvascular blood flow of contrast-enhanced ultrasound and HGF/c-Met signaling pathway with clinicopathological features and prognosis of patients with hepatocellular carcinoma. Onco Targets Ther. 10:847–857. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Bouattour M, Raymond E, Qin S, Cheng AL, Stammberger U, Locatelli G and Faivre S: Recent developments of c-Met as a therapeutic target in hepatocellular carcinoma. Hepatology. 67:1132–1149. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Kim JH, Kim HS, Kim BJ, Jang HJ and Lee J: Prognostic value of c-Met overexpression in hepatocellular carcinoma: A meta-analysis and review. Oncotarget. 8:90351–90357. 2017.PubMed/NCBI

27 

Yan S, Jiao X, Zou H and Li K: Prognostic significance of c-Met in breast cancer: A meta-analysis of 6010 cases. Diagn Pathol. 10:622015. View Article : Google Scholar : PubMed/NCBI

28 

Pyo JS, Kang G, Cho WJ and Choi SB: Clinicopathological significance and concordance analysis of c-MET immunohistochemistry in non-small cell lung cancers: A meta-analysis. Pathol Res Pract. 212:710–716. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Liu Y, Yu XF, Zou J and Luo ZH: Prognostic value of c-Met in colorectal cancer: A meta-analysis. World J Gastroenterol. 21:3706–3710. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Xin Y, Jin D, Eppler S, Damico-Beyer LA, Joshi A, Davis JD, Kaur S, Nijem I, Bothos J, Peterson A, et al: Population pharmacokinetic analysis from phase I and phase II studies of the humanized monovalent antibody, onartuzumab (MetMAb), in patients with advanced solid tumors. J Clin Pharmacol. 53:1103–1111. 2013.PubMed/NCBI

31 

Lohitesh K, Chowdhury R and Mukherjee S: Resistance a major hindrance to chemotherapy in hepatocellular carcinoma: An insight. Cancer Cell Int. 18:442018. View Article : Google Scholar : PubMed/NCBI

32 

Zhang Q, Zhou M, Wu X, Li Z, Liu B, Gao W, Yue J and Liu T: Promoting therapeutic angiogenesis of focal cerebral ischemia using thrombospondin-4 (TSP4) gene-modified bone marrow stromal cells (BMSCs) in a rat model. J Trans Med. 17:1112019. View Article : Google Scholar

33 

Gandara C, Affleck V and Stoll EA: Manufacture of third- generation lentivirus for preclinical use, with process development considerations for translation to good manufacturing practice. Hum Gene Ther Methods. 29:1–15. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Wang YF, Kunda PE, Lin JW, Wang H, Chen XM, Liu QL and Liu T: Cytokine-induced killer cells co-cultured with complete tumor antigen-loaded dendritic cells, have enhanced selective cytotoxicity on carboplatin-resistant retinoblastoma cells. Oncol Rep. 29:1841–1850. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Jackson HJ and Brentjens RJ: Overcoming antigen escape with CAR T-cell therapy. Cancer Discov. 5:1238–1240. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Harjes U: CAR antigens beyond recognition. Nat Rev Cancer. 18:7232018. View Article : Google Scholar : PubMed/NCBI

37 

Zhang Q, Bi J, Zheng X, Chen Y, Wang H, Wu W, Wang Z, Wu Q, Peng H, Wei H, et al: Blockade of the checkpoint receptor TIGIT prevents NK cell exhaustion and elicits potent anti-tumor immunity. Nature immunology. 19:723–732. 2018. View Article : Google Scholar : PubMed/NCBI

38 

Orlando EJ, Han X, Tribouley C, Wood PA, Leary RJ, Riester M, Levine JE, Qayed M, Grupp SA, Boyer M, et al: Genetic mechanisms of target antigen loss in CAR19 therapy of acute lymphoblastic leukemia. Nat Med. 24:1504–1506. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Li Y, Hermanson DL, Moriarity BS and Kaufman DS: Human iPSC-derived natural killer cells engineered with chimeric antigen receptors enhance anti-tumor activity. Cell Stem Cell. 23:181–192, e185. 2018. View Article : Google Scholar : PubMed/NCBI

40 

Oelsner S, Waldmann A, Billmeier A, Röder J, Lindner A, Ullrich E, Marschalek R, Dotti G, Jung G, Grosse-Hovest L, et al: Genetically engineered CAR NK cells display selective cytotoxicity against FLT3-positive B-ALL and inhibit in vivo leukemia growth. Int J Cancer. Mar 13–2019.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

41 

Shiozawa M, Chang CH, Huang YC, Chen YC, Chi MS, Hao HC, Chang YC, Takeda S, Chi KH and Wang YS: Pharmacologically upregulated carcinoembryonic antigen-expression enhances the cytolytic activity of genetically-modified chimeric antigen receptor NK-92MI against colorectal cancer cells. BMC Immunol. 19:272018. View Article : Google Scholar : PubMed/NCBI

42 

Escors D and Breckpot K: Lentiviral vectors in gene therapy: Their current status and future potential. Arch Immunol Ther Exp (Warsz). 58:107–119. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Schambach A, Zychlinski D, Ehrnstroem B and Baum C: Biosafety features of lentiviral vectors. Hum Gene Ther. 24:132–142. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu B, Liu ZZ, Zhou ML, Lin JW, Chen XM, Li Z, Gao WB, Yu ZD and Liu T: Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells. Mol Med Rep 20: 2823-2831, 2019.
APA
Liu, B., Liu, Z., Zhou, M., Lin, J., Chen, X., Li, Z. ... Liu, T. (2019). Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells. Molecular Medicine Reports, 20, 2823-2831. https://doi.org/10.3892/mmr.2019.10529
MLA
Liu, B., Liu, Z., Zhou, M., Lin, J., Chen, X., Li, Z., Gao, W., Yu, Z., Liu, T."Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells". Molecular Medicine Reports 20.3 (2019): 2823-2831.
Chicago
Liu, B., Liu, Z., Zhou, M., Lin, J., Chen, X., Li, Z., Gao, W., Yu, Z., Liu, T."Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells". Molecular Medicine Reports 20, no. 3 (2019): 2823-2831. https://doi.org/10.3892/mmr.2019.10529
Copy and paste a formatted citation
x
Spandidos Publications style
Liu B, Liu ZZ, Zhou ML, Lin JW, Chen XM, Li Z, Gao WB, Yu ZD and Liu T: Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells. Mol Med Rep 20: 2823-2831, 2019.
APA
Liu, B., Liu, Z., Zhou, M., Lin, J., Chen, X., Li, Z. ... Liu, T. (2019). Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells. Molecular Medicine Reports, 20, 2823-2831. https://doi.org/10.3892/mmr.2019.10529
MLA
Liu, B., Liu, Z., Zhou, M., Lin, J., Chen, X., Li, Z., Gao, W., Yu, Z., Liu, T."Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells". Molecular Medicine Reports 20.3 (2019): 2823-2831.
Chicago
Liu, B., Liu, Z., Zhou, M., Lin, J., Chen, X., Li, Z., Gao, W., Yu, Z., Liu, T."Development of c‑MET‑specific chimeric antigen receptor‑engineered natural killer cells with cytotoxic effects on human liver cancer HepG2 cells". Molecular Medicine Reports 20, no. 3 (2019): 2823-2831. https://doi.org/10.3892/mmr.2019.10529
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team