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MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data

  • Authors:
    • Hongshi Cao
    • Yu Zhang
    • Zhe Chu
    • Bolun Zhao
    • Haiyan Wang
    • Libin An
  • View Affiliations / Copyright

    Affiliations: School of Nursing, Jilin University, Jilin 130021, P.R. China, Department of Neurovascular Disease, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Emergency, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, School of Nursing, Dalian University, Dalian, Liaoning 116000, P.R. China, Department of Neurotrauma Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3011-3018
    |
    Published online on: July 30, 2019
       https://doi.org/10.3892/mmr.2019.10538
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Abstract

Spinal cord injury (SCI) is a specific type of damage to the central nervous system causing temporary or permanent changes in its function. The present aimed to identify the genetic changes in neuroplasticity following SCI in rats. The GSE52763 microarray dataset, which included 15 samples [3 sham (1 week), 4 injury only (1 week), 4 injury only (3 weeks), 4 injury + treadmill (3 weeks)] was downloaded from the Gene Expression Omnibus database. An empirical Bayes linear regression model in limma package was used to identify the differentially expressed genes (DEGs) in injury vs. sham and treadmill vs. non‑treadmill comparison groups. Subsequently, time series and enrichment analyses were performed using pheatmap and clusterProfile packages, respectively. Additionally, protein‑protein interaction (PPI) and transcription factor (TF)‑microRNA (miRNA)‑target regulatory networks were constructed using Cytoscape software. In total, 159 and 105 DEGs were identified in injury vs. sham groups and treadmill vs. non‑treadmill groups, respectively. There were 40 genes in cluster 1 that presented increased expression levels in the injury (1 week/3 weeks) groups compared with the sham group, and decreased expression levels in the injury + treadmill group compared with the injury only groups; conversely, 52 genes in cluster 2 exhibited decreased expression levels in the injury (1 week/3 weeks) groups compared with the sham group, and increased expression levels in the injury + treadmill group compared with the injury only groups. Enrichment analysis indicated that clusters 1 and 2 were associated with immune response and signal transduction, respectively. Furthermore, microtubule associated protein 1B, phosphofurin acidic cluster sorting protein 2 and adenosylhomocysteinase‑like 1 exhibited the highest degrees in the regulatory network, and were regulated by miRNAs including miR‑34A, miR‑34B, miR‑34C and miR‑449. These miRNAs and their target genes may serve important roles in neuroplasticity following traumatic SCI in rats. Nevertheless, additional in‑depth studies are required to confirm these data.
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Copy and paste a formatted citation
Spandidos Publications style
Cao H, Zhang Y, Chu Z, Zhao B, Wang H and An L: MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data. Mol Med Rep 20: 3011-3018, 2019.
APA
Cao, H., Zhang, Y., Chu, Z., Zhao, B., Wang, H., & An, L. (2019). MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data. Molecular Medicine Reports, 20, 3011-3018. https://doi.org/10.3892/mmr.2019.10538
MLA
Cao, H., Zhang, Y., Chu, Z., Zhao, B., Wang, H., An, L."MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data". Molecular Medicine Reports 20.4 (2019): 3011-3018.
Chicago
Cao, H., Zhang, Y., Chu, Z., Zhao, B., Wang, H., An, L."MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data". Molecular Medicine Reports 20, no. 4 (2019): 3011-3018. https://doi.org/10.3892/mmr.2019.10538
Copy and paste a formatted citation
x
Spandidos Publications style
Cao H, Zhang Y, Chu Z, Zhao B, Wang H and An L: MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data. Mol Med Rep 20: 3011-3018, 2019.
APA
Cao, H., Zhang, Y., Chu, Z., Zhao, B., Wang, H., & An, L. (2019). MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data. Molecular Medicine Reports, 20, 3011-3018. https://doi.org/10.3892/mmr.2019.10538
MLA
Cao, H., Zhang, Y., Chu, Z., Zhao, B., Wang, H., An, L."MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data". Molecular Medicine Reports 20.4 (2019): 3011-3018.
Chicago
Cao, H., Zhang, Y., Chu, Z., Zhao, B., Wang, H., An, L."MAP‑1B, PACS‑2 and AHCYL1 are regulated by miR‑34A/B/C and miR‑449 in neuroplasticity following traumatic spinal cord injury in rats: Preliminary explorative results from microarray data". Molecular Medicine Reports 20, no. 4 (2019): 3011-3018. https://doi.org/10.3892/mmr.2019.10538
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