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Article Open Access

Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy

  • Authors:
    • Yu‑Hui Li
    • Wei‑Li Zhang
    • Hao‑Ying Zhou
    • Da‑Wei Yu
    • Xiao‑Ning Sun
    • Qin Hu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Cardiology, Yantaishan Hospital, Yantai, Shandong 264001, P.R. China, Department of Thoracic Surgery, People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, P.R. China, Department of Nephrology, Yantaishan Hospital, Yantai, Shandong 264001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3131-3139
    |
    Published online on: August 6, 2019
       https://doi.org/10.3892/mmr.2019.10554
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Abstract

Advanced glycation end products (AGEs) have been reported to serve an important role in the stiffening of cardiac tissues and myocardial cell injury. Serious myocardial cell injury can result in various heart diseases with high mortality. Halofuginone (HF), which possesses marked anti‑inflammatory and antifibrotic effects, has recently been applied to inhibit the effects of cardiac stress. The present study aimed to investigate the potential effects of HF and its underlying mechanism in the treatment of AGEs‑induced H9C2 cardiomyocyte damage. The western blot results of the present study demonstrated that HF may reduce the expression levels of myocardial injury markers, including myoglobin, creatine kinase MB and cardiac troponin I. In addition, flow cytometric analysis indicated that the production of reactive oxygen species (ROS) was significantly decreased by HF. Additionally, endoplasmic reticulum (ER) stress was suppressed in response to treatment with HF, as observed by low expression levels of ER stress‑associated proapoptotic proteins (CCAAT/enhancer‑binding protein homologous protein and cleaved caspase‑12); overexpression of prosurvival proteins (growth arrest and DNA damage‑inducible protein GADD34 and binding immunoglobulin protein) was also reported. Furthermore, the expression levels of microtubule‑associated proteins 1A/1B light chain 3B (LC3)II/LC3I and Beclin 1 were elevated, whereas P62 expression levels were reduced following treatment with HF. These findings, together with immunofluorescence staining of LC3, indicated that HF may induce autophagy. Finally, the protective effects of HF on AGEs‑treated H9C2 cells were reversed following treatment with the inhibitor 3‑methyladenine, as indicated by inhibition of autophagy, and increases in apoptosis, ROS production and the ER stress response. Collectively, the findings of the present study suggested that the protective effects of HF against AGEs‑induced myocardial cell injury may be associated with the induction of autophagy and amelioration of ROS‑mediated ER stress and apoptosis. These findings may contribute to the development of a novel therapeutic method to inhibit the progression of myocardial cell injury.
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Copy and paste a formatted citation
Spandidos Publications style
Li YH, Zhang WL, Zhou HY, Yu DW, Sun XN and Hu Q: Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy. Mol Med Rep 20: 3131-3139, 2019.
APA
Li, Y., Zhang, W., Zhou, H., Yu, D., Sun, X., & Hu, Q. (2019). Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy. Molecular Medicine Reports, 20, 3131-3139. https://doi.org/10.3892/mmr.2019.10554
MLA
Li, Y., Zhang, W., Zhou, H., Yu, D., Sun, X., Hu, Q."Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy". Molecular Medicine Reports 20.4 (2019): 3131-3139.
Chicago
Li, Y., Zhang, W., Zhou, H., Yu, D., Sun, X., Hu, Q."Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy". Molecular Medicine Reports 20, no. 4 (2019): 3131-3139. https://doi.org/10.3892/mmr.2019.10554
Copy and paste a formatted citation
x
Spandidos Publications style
Li YH, Zhang WL, Zhou HY, Yu DW, Sun XN and Hu Q: Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy. Mol Med Rep 20: 3131-3139, 2019.
APA
Li, Y., Zhang, W., Zhou, H., Yu, D., Sun, X., & Hu, Q. (2019). Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy. Molecular Medicine Reports, 20, 3131-3139. https://doi.org/10.3892/mmr.2019.10554
MLA
Li, Y., Zhang, W., Zhou, H., Yu, D., Sun, X., Hu, Q."Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy". Molecular Medicine Reports 20.4 (2019): 3131-3139.
Chicago
Li, Y., Zhang, W., Zhou, H., Yu, D., Sun, X., Hu, Q."Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy". Molecular Medicine Reports 20, no. 4 (2019): 3131-3139. https://doi.org/10.3892/mmr.2019.10554
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