RAD18 contributes to the migration and invasion of human cervical cancer cells via the interleukin‑1β pathway

Lou,Pengrong; Zou,Shitao; Shang,Zengfu; He,Chao; Gao,Aidi; Hou,Shunyu; Zhou,Jundong

doi:10.3892/mmr.2019.10564

RAD18 contributes to the migration and invasion of human cervical cancer cells via the interleukin‑1β pathway

Authors:
- Pengrong Lou
- Shitao Zou
- Zengfu Shang
- Chao He
- Aidi Gao
- Shunyu Hou
- Jundong Zhou
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Affiliations: Center of Chemoradio‑Oncology, Ningbo First Hospital, Ningbo, Zhejiang 315010, P.R. China, Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China, School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, Jiangsu 215123, P.R. China, Department of Gynecology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China
Published online on: August 6, 2019 https://doi.org/10.3892/mmr.2019.10564
Pages: 3415-3423

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Abstract

The E3 ubiquitin ligase RAD18 has been identified as an oncoprotein that exhibits prometastatic properties in various types of cancer; however, the role of RAD18 in cervical cancer (CC) remains unclear. In the present study, it was revealed that increased expression of RAD18 was associated with worse prognosis of patients with CC. Knockdown of endogenous RAD18 suppressed the motility and invasiveness of CC cells, as evaluated by Transwell assays. mRNA sequencing revealed that silencing RAD18 altered the expression profile of proinflammatory mediators, such as interleukin‑1β (IL‑1β). Furthermore, exogenous IL‑1β treatment rescued RAD18‑mediated CC cell invasion. These findings indicated an underlying mechanism via which RAD18 promotes CC progression, suggesting that RAD18 may be a potential biomarker and therapeutic target for malignant CC.

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