Cisplatin decreases cyclin D2 expression via upregulating miR‑93 to inhibit lung adenocarcinoma cell growth

Xie,Ning; Liu,Yuan‑Rong; Li,Yan‑Mei; Yang,Ya‑Nan; Pan,Li; Wei,Yu‑Bo; Wang,Ping‑Yu; Li,You‑Jie; Xie,Shu‑Yang

doi:10.3892/mmr.2019.10566

Cisplatin decreases cyclin D2 expression via upregulating miR‑93 to inhibit lung adenocarcinoma cell growth

Authors:
- Ning Xie
- Yuan‑Rong Liu
- Yan‑Mei Li
- Ya‑Nan Yang
- Li Pan
- Yu‑Bo Wei
- Ping‑Yu Wang
- You‑Jie Li
- Shu‑Yang Xie
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Affiliations: Department of Chest Surgery, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China, Department of Biochemistry and Molecular Biology, Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Binzhou Medical University, Yantai, Shandong 264003, P.R. China
Published online on: August 6, 2019 https://doi.org/10.3892/mmr.2019.10566
Pages: 3355-3362
Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) serve important roles in the chemotherapeutic effect of anticancer drugs. To investigate the roles of miRNAs in cisplatin‑induced suppression of lung adenocarcinoma cell proliferation, A549 cells were treated with different concentrations of cisplatin. An MTT assay demonstrated that cisplatin inhibited A549 cell proliferation in a dose‑dependent manner. Cisplatin induced cell apoptosis and inhibited cell migration by increasing the levels of miR‑93, miR‑26a and miR‑26b. Furthermore, as an upstream factor, miR‑93 was proposed to regulate cyclin D2 expression in miR‑93‑transfected A549 cells. Cisplatin also induced Bcl‑2‑associated X protein expression, and decreased that of Bcl‑2 and c‑Myc in lung adenocarcinoma cells. In vivo analysis further supported that cisplatin inhibited lung adenocarcinoma cell growth by regulating cyclin D2 and miR‑93 expression. In conclusion, our findings demonstrated that cisplatin could effectively inhibit lung adenocarcinoma cell proliferation by decreasing cyclin D2 expression via miR‑93.

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