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Article

Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival

  • Authors:
    • Fengju Li
    • Dingran Si
    • Xuejun Guo
    • Ningru Guo
    • Dandan Li
    • Liujing Zhang
    • Xianan Jian
    • Jiasheng Ma
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatism and Immunology, Puyang Oilfield General Hospital, Puyang, Henan 457001, P.R. China, Department of Cardiovascular Medicine, Puyang Oilfield General Hospital, Puyang, Henan 457001, P.R. China, Department of Hematology, Puyang Oilfield General Hospital, Puyang, Henan 457001, P.R. China
  • Pages: 3388-3394
    |
    Published online on: August 8, 2019
       https://doi.org/10.3892/mmr.2019.10573
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Abstract

Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease. MicroRNAs (miRNAs) are a group of endogenous small non‑coding RNAs that regulate target genes, and play a critical role in many biological processes. However, the underlying mechanism of specific miRNA, miR‑130a‑3p, in AS remains largely unknown. Therefore, the present study aimed to explore the underlying mechanism of miR‑130a‑3p in the development of AS. In the present study, it was revealed that miR‑130a‑3p was downregulated in T cells from HLA‑B27‑positive AS patients compared with the HLA‑B27‑negative healthy controls. Next, bioinformatics software TargetScan 7.2 was used to predict the target genes of miR‑130a‑3p, and a luciferase reporter assay indicated that HOXB1 was the direct target gene of miR‑130a‑3p. Furthermore, it was determined that HOXB1 expression was upregulated in T cells from HLA‑B27‑positive AS patients. In addition, the results of the present study indicated that miR‑130a‑3p inhibitor significantly inhibited cell proliferation ability and induced cell apoptosis of Jurkat T cells, while the miR‑130a‑3p mimic promoted proliferation ability and inhibited cell apoptosis of Jurkat T cells. Notably, all the effects of the miR‑130a‑3p mimic on Jurkat T cells were reversed by HOXB1‑plasmid. Collectively, our data indicated that miR‑130a‑3p was decreased in T cells from AS patients and it could regulate T‑cell survival by targeting HOXB1.
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View References

1 

Dougados M and Baeten D: Sphodyloarthritis. Lancet. 377:2127–2137. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Evans DM, Spencer CC, Pointon JJ, Su Z, Harvey D, Kochan G, Oppermann U, Dilthey A, Pirinen M, Stone MA, et al: Interaction between ERAP1 and HLA-B27 in ankylosing spondylitis implicates peptide handling in the mechanism for HLA-B27 in disease susceptibility. Nat Genet. 43:761–767. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Hou ZD, Xiao ZY, Gong Y, Zhang YP and Zeng QY: Arylamine N-acetyltransferase polymorphisms in Han Chinese patients with ankylosing spondylitis and their correlation to the adverse drug reactions to sulfasalazine. BMC Pharmacol Toxicol. 15:64–80. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Liu Z, Zhang P and Dong J: Genetic variants of STAT4 are associated with ankylosing spondylitis susceptibility and severity in a Chinese Han population. Int J Clin Exp Med. 7:5877–5881. 2014.PubMed/NCBI

5 

Dean LE, Jones GT, MacDonald AG, Downham C, Sturrock RD and Macfarlane GJ: Global prevalence of ankylosing spondylitis. Rheumatology (Oxford). 53:650–657. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Haywood KL, Packham JC and Jordan KP: Assessing fatigue in ankylosing spondylitis: The importance of frequency and severity. Rheumatology. 53:552–556. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Gan FY, Fei YY, Li MT, Wang Q, Xu D, Hou Y, Zeng XF and Zhang FC: The characteristics of patients having ankylosing spondylitis associated with Takayasu's arteritis. Clin Rheumatol. 33:355–358. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Li X, Liu F, Lin B, Luo H, Liu M, Wu J, Li C, Li R, Zhang X, Zhou K and Ren D: miR-150 inhibits proliferation and tumorigenicity via retarding G1/S phase transition in nasopharyngeal carcinoma. Int J Oncol. 50:1097–1108. 2017. View Article : Google Scholar :

9 

Ro S, Park C, Young D, Sanders KM and Yan W: Tissue-dependent paired expression of miRNAs. Nucleic Acids Res. 35:5944–5953. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Mallory AC and Vaucheret H: MicroRNAs: Something important between the genes. Curr Opin Plant Biol. 7:120–125. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Garzon R, Calin GA and Croce CM: MicroRNAs in cancer. Annu Rev Med. 60:167–179. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Gaur N, Karouzakis E, Gluck S, Bagdonas E, Jüngel A, Michel BA, Gay RE, Gay S, Frank-Bertoncelj M and Neidhart M: Micrornas interfere with DNA methylation in rheumatoid arthritis synovial fibroblasts. RMD Open. 2:e0002992016. View Article : Google Scholar : PubMed/NCBI

13 

Freiesleben S, Hecker M, Zettl UK, Fuellen G and Taher L: Analysis of microRNA and gene expression profiles in multiple sclerosis: Integrating interaction data to uncover regulatory mechanisms. Sci Rep. 6:345122016. View Article : Google Scholar : PubMed/NCBI

14 

Lai NS, Yu HC, Chen HC, Yu CL, Huang HB and Lu MC: Aberrant expression of microRNAs in T cells from patients with ankylosing spondylitis contributes to the immunopathogenesis. Clin Exp Immunol. 173:47–57. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Jiang Y and Wang L: Role of histone deacetylase 3 in ankylosing spondylitis via negative feedback loop with microRNA-130a and enhancement of tumor necrosis factor-1α expression in peripheral blood mononuclear cells. Mol Med Rep. 13:35–40. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Shah N and Sukumar S: The Hox genes and their roles in oncogenesis. Nat Rev Cancer. 10:361–371. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Han L, Liu D, Li Z, Tian N, Han Z, Wang G, Fu Y, Guo Z, Zhu Z, Du C and Tian Y: HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma. PLoS One. 10:e01423872015. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the (-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Su JR, Kuai JH and Li YQ: Smoc2 potentiates proliferation of hepatocellular carcinoma cells via promotion of cellcycle progression. World J Gastroenterol. 22:10053–10063. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Jiang Y, Wang W, Liu ZY, Xie Y, Qian Y and Cai XN: Overexpression of miR-130a-3p/301a-3p attenuates high glucose-induced MPC5podocyte dysfunction through suppression of TNF-α signaling. Exp Ther Med. 15:1021–1028. 2018.PubMed/NCBI

21 

Appel H, Maier R, Bleil J, Hempfing A, Loddenkemper C, Schlichting U, Syrbe U and Sieper J: In situ analysis of interleukin-23-and interleukin-12-positive cells in the spine of patients with ankylosing spondylitis. Arthritis Rheum. 65:1522–1529. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Syrbe U, Scheer R, Wu P and Sieper J: Differential synovial Th1 cell reactivity towards Escherichia coli antigens in patients with ankylosing spondylitis and rheumatoid arthritis. Ann Rheum Dis. 71:1573–1576. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Wang Y, Luo J, Wang X, Yang B and Cui L: MicroRNA-199a-5p induced autophagy and inhibits the pathogenesis of ankylosing spondylitis by modulating the mTOR signaling via directly targeting ras homolog enriched in brain (Rheb). Cell Physiol Biochem. 42:24812017. View Article : Google Scholar : PubMed/NCBI

24 

Hou C, Zhu M, Sun M and Lin Y: MicroRNA let-7i induced autophagy to protect T cell from apoptosis by targeting IGF1R. Biochem Biophys Res Commun. 453:728–734. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Zou J, Appel H, Rudwaleit M, Thiel A and Sieper J: Analysis of the CD8+ T cell response to the G1 domain of aggrecan in ankylosing spondylitis. Ann Rheum Dis. 64:722–729. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Wu Y, Ren M, Yang R, Liang X, Ma Y, Tang Y, Huang L, Ye J, Chen K, Wang P and Shen H: Reduced immunomodulation potential of bone marrow-derived mesenchymal stem cells induced CCR4+CCR6+ Th/Treg cell subset imbalance in ankylosing spondylitis. Arthritis Res Ther. 13:R292011. View Article : Google Scholar : PubMed/NCBI

27 

Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P and Wedderburn LR: Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers. Arthritis Rheum. 58:875–887. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Reed JC: Bcl-2 and the regulation of programmed cell death. J Cell Biol. 124:1–6. 1994. View Article : Google Scholar : PubMed/NCBI

29 

Hatok J and Racay P: Bcl-2 family proteins: Master regulators of cell survival. Biomol Concepts. 7:259–270. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Thomas S, Quinn BA, Das SK, Dash R, Emdad L, Dasgupta S, Wang XY, Dent P, Reed JC, Pellecchia M, et al: Targeting the Bcl-2 family for cancer therapy. Expert Opin Ther Targets. 17:61–75. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Gross A, Jockel J, Wei MC and Korsmeyer SJ: Enforced dimerization of BAX results in its translocation, mitochondrial dysfunction and apoptosis. EMBO J. 17:3878–3885. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Petrini M, Felicetti F, Bottero L, Errico MC, Morsilli O, Boe A, De Feo A and Carè A: HOXB1 restored expression promotes apoptosis and differentiation in the HL60 leukemic cell line. Cancer Cell Int. 13:1012013. View Article : Google Scholar : PubMed/NCBI

33 

Staton AA and Giraldez AJ: Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo. Nat Protoc. 6:2035–2049. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Knauss JL, Bian S and Sun T: Plasmid-based target protectors allow specific blockade of miRNA silencing activity in mammalian developmental systems. Front Cell Neurosci. 7:1632013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li F, Si D, Guo X, Guo N, Li D, Zhang L, Jian X and Ma J: Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival. Mol Med Rep 20: 3388-3394, 2019.
APA
Li, F., Si, D., Guo, X., Guo, N., Li, D., Zhang, L. ... Ma, J. (2019). Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival. Molecular Medicine Reports, 20, 3388-3394. https://doi.org/10.3892/mmr.2019.10573
MLA
Li, F., Si, D., Guo, X., Guo, N., Li, D., Zhang, L., Jian, X., Ma, J."Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival". Molecular Medicine Reports 20.4 (2019): 3388-3394.
Chicago
Li, F., Si, D., Guo, X., Guo, N., Li, D., Zhang, L., Jian, X., Ma, J."Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival". Molecular Medicine Reports 20, no. 4 (2019): 3388-3394. https://doi.org/10.3892/mmr.2019.10573
Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Si D, Guo X, Guo N, Li D, Zhang L, Jian X and Ma J: Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival. Mol Med Rep 20: 3388-3394, 2019.
APA
Li, F., Si, D., Guo, X., Guo, N., Li, D., Zhang, L. ... Ma, J. (2019). Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival. Molecular Medicine Reports, 20, 3388-3394. https://doi.org/10.3892/mmr.2019.10573
MLA
Li, F., Si, D., Guo, X., Guo, N., Li, D., Zhang, L., Jian, X., Ma, J."Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival". Molecular Medicine Reports 20.4 (2019): 3388-3394.
Chicago
Li, F., Si, D., Guo, X., Guo, N., Li, D., Zhang, L., Jian, X., Ma, J."Aberrant expression of miR‑130a‑3p in ankylosing spondylitis and its role in regulating T‑cell survival". Molecular Medicine Reports 20, no. 4 (2019): 3388-3394. https://doi.org/10.3892/mmr.2019.10573
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