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Article

Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney

  • Authors:
    • Chenguang Ding
    • Feng Han
    • Heli Xiang
    • Yuxiang Wang
    • Meng Dou
    • Xinxin Xia
    • Yang Li
    • Jin Zheng
    • Xiaoming Ding
    • Wujun Xue
    • Puxun Tian
  • View Affiliations / Copyright

    Affiliations: Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 3337-3346
    |
    Published online on: August 8, 2019
       https://doi.org/10.3892/mmr.2019.10576
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Abstract

The mechanisms by which prostaglandin E2 receptor 4 (EP4) protects against renal ischemia‑reperfusion (I/R) injury (IRI) remain to be fully elucidated. In the present study, the protective effects of EP4 signaling on renal mitochondria and against renal IRI, as well as the underlying mechanisms, were investigated. A rat model of renal IRI was established. The right kidney was separated without damaging the artery clip, and the renal blood perfusion was then restored after 60 min. One group of animals was treated with EP4 agonists prior to I/R. The mitochondrial mass, the copy number of mitochondrial (mt)DNA, adenosine triphosphate (ATP) production and mitochondrial autophagy were analyzed. It was identified that renal IRI reduced the mitochondrial mass, decreased the mtDNA copy number and inhibited ATP production. The loss of renal mitochondria was attributed to the excessive mitochondrial autophagy induced by renal IRI. Pre‑treatment with EP4 agonist inhibited excessive mitochondrial autophagy, the loss of mitochondria and maintained and the energy imbalance within the cells. It was indicated that renal IRI causes excessive mitochondrial autophagy, which is one of the important causes of renal dysfunction.
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Copy and paste a formatted citation
Spandidos Publications style
Ding C, Han F, Xiang H, Wang Y, Dou M, Xia X, Li Y, Zheng J, Ding X, Xue W, Xue W, et al: Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney . Mol Med Rep 20: 3337-3346, 2019.
APA
Ding, C., Han, F., Xiang, H., Wang, Y., Dou, M., Xia, X. ... Tian, P. (2019). Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney . Molecular Medicine Reports, 20, 3337-3346. https://doi.org/10.3892/mmr.2019.10576
MLA
Ding, C., Han, F., Xiang, H., Wang, Y., Dou, M., Xia, X., Li, Y., Zheng, J., Ding, X., Xue, W., Tian, P."Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney ". Molecular Medicine Reports 20.4 (2019): 3337-3346.
Chicago
Ding, C., Han, F., Xiang, H., Wang, Y., Dou, M., Xia, X., Li, Y., Zheng, J., Ding, X., Xue, W., Tian, P."Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney ". Molecular Medicine Reports 20, no. 4 (2019): 3337-3346. https://doi.org/10.3892/mmr.2019.10576
Copy and paste a formatted citation
x
Spandidos Publications style
Ding C, Han F, Xiang H, Wang Y, Dou M, Xia X, Li Y, Zheng J, Ding X, Xue W, Xue W, et al: Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney . Mol Med Rep 20: 3337-3346, 2019.
APA
Ding, C., Han, F., Xiang, H., Wang, Y., Dou, M., Xia, X. ... Tian, P. (2019). Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney . Molecular Medicine Reports, 20, 3337-3346. https://doi.org/10.3892/mmr.2019.10576
MLA
Ding, C., Han, F., Xiang, H., Wang, Y., Dou, M., Xia, X., Li, Y., Zheng, J., Ding, X., Xue, W., Tian, P."Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney ". Molecular Medicine Reports 20.4 (2019): 3337-3346.
Chicago
Ding, C., Han, F., Xiang, H., Wang, Y., Dou, M., Xia, X., Li, Y., Zheng, J., Ding, X., Xue, W., Tian, P."Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney ". Molecular Medicine Reports 20, no. 4 (2019): 3337-3346. https://doi.org/10.3892/mmr.2019.10576
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