TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production

Fan,Chun; Ji,Qiuxia; Zhang,Chunyang; Xu,Shuo; Sun,Hui; Li,Zhiyuan

doi:10.3892/mmr.2019.10580

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production

Authors:
- Chun Fan
- Qiuxia Ji
- Chunyang Zhang
- Shuo Xu
- Hui Sun
- Zhiyuan Li
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Affiliations: Department of Periodontology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, P.R. China, Key Laboratory, Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, P.R. China
Published online on: August 9, 2019 https://doi.org/10.3892/mmr.2019.10580
Pages: 3123-3130
Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Periodontal ligament stem cells (PDLSCs) are vital for the regeneration of periodontal tissue. Transforming growth factor (TGF) β1, a potent stimulator of tissue regeneration, is extensive and abundant in the bone matrix. However, the effect of TGF‑β1 in periodontal differentiation remains to be elucidated. The present study aimed to evaluate the effect of TGF‑β1 on human PDLSCs. PDLSCs were isolated using CD146 microbeads, and characterized by flow cytometry. The present study demonstrated that treatment with TGF‑β1 induced PDLSC senescence, characterized by increases in senescence‑associated beta‑galactosidase activity and elevation of both p16 and p21 expression. Furthermore, TGF‑β1 treatment demonstrated the capacity to induce the production of reactive oxygen species (ROS). Of note, addition of a ROS scavenger successfully rescued the TGF‑β1‑induced PDLSC senescence. Thus, the present results indicated that TGF‑β1 may serve a vital role in PDLSC senescence, and thus represent a potential target involved in the fabrication and formation of hard tissue for clinical treatment.

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