Synergistic effect of STAT3‑targeted small interfering RNA and AZD0530 against glioblastoma in vitro and in vivo

Liu,Qingjun; Wang,Leibo; Li,David; Zhao,Jingxia; Chen,Shen; Li,Jialin

doi:10.3892/mmr.2019.10596

Synergistic effect of STAT3‑targeted small interfering RNA and AZD0530 against glioblastoma in vitro and in vivo

Authors:
- Qingjun Liu
- Leibo Wang
- David Li
- Jingxia Zhao
- Shen Chen
- Jialin Li
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Affiliations: Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300050, P.R. China, English Department, International Medical School, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Physiology, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Neurosurgery, Hebei United University, Tangshan, Hebei 063000, P.R. China
Published online on: August 21, 2019 https://doi.org/10.3892/mmr.2019.10596
Pages: 3625-3632
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of this study was to explore the synergistic effect of signal transducer and activator of transcription 3 (STAT3)‑targeted small interfering (si)RNA and AZD0530 against glioblastoma in vitro and in vivo. Glioblastoma cell lines U87 and U251 were divided into four groups and treated with control, LV‑STAT3 siRNA, AZD0530, and combined LV‑STAT3 siRNA with AZD0530, respectively. The proliferation and apoptotic capacity of glioblastoma cells was assessed by Cell Counting Kit‑8 and double staining flow cytometry assays, respectively. Additionally, the potential effect of LV‑STAT3 siRNA and AZD0530 on glioblastoma was evaluated in vivo. Images were captured of the tumor formation in mice every week. Following three weeks of treatment, NMR scan and immunohistochemistry were performed. The treatment of combined LV‑STAT3 siRNA and AZD0530 was more effective in inhibiting proliferation and inducing apoptosisof glioblastoma cells in comparison with the treatment of either LV‑STAT3 siRNA or AZD0530 alone. Although LV‑STAT3 siRNA or AZD0530 treatment alone suppressed tumor growth in mice, the combined treatment had a more significant effect than the treatment of LV‑STAT3 siRNA or AZD0530 alone. According to the results of both in vitro and in vivo assays, a combined therapy of LV‑STAT3 siRNA with AZD0530 could enhance therapeutic effects on glioblastoma, supporting the idea that the combination of LV‑STAT3 siRNA and AZD0530 could serve as a novel and effective strategy to combat glioblastoma.

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