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Article

Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis

  • Authors:
    • Lei‑Lei Gao
    • Fei Xu
    • Zhen Jin
    • Xiao‑Yan Ying
    • Jin‑Wei Liu
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, P.R. China, Department of Gynecology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China, Reproductive Genetic Center, Suzhou Municipal Hospital, Suzhou Hospital of Nanjing, Nanjing, Jiangsu 215000, P.R. China, Department of Gynecology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210011, P.R. China
  • Pages: 3573-3582
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    Published online on: August 22, 2019
       https://doi.org/10.3892/mmr.2019.10605
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Abstract

Microtubule‑severing proteins (MTSPs) are a group of microtubule‑associated proteins essential for multiple microtubule‑related processes, including mitosis and meiosis. Katanin p60 ATPase‑containing subunit A‑like 1 (p60 katanin‑like 1) is an MTSP that maintains the density of spindle microtubules at the poles in mitotic cells; however, to date, there have been no studies about its role in female meiosis. Using in vitro‑matured (IVM) oocytes as a model, it was first revealed that p60 katanin‑like 1 was predominant in the ovaries and oocytes, indicating its essential roles in oocyte meiosis. It was also revealed that p60 katanin‑like 1 was concentrated at the spindle poles and co‑localized and interacted with γ‑tubulin, indicating that it may be involved in pole organization. Next, specific siRNA was used to deplete p60 katanin‑like 1; the spindle organization was severely disrupted and characterized by an abnormal width:length ratio, multipolarity and extra aster microtubules out of the main spindles. Finally, it was determined that p60 katanin‑like 1 knockdown retarded oocyte meiosis, reduced fertilization, and caused abnormal mitochondrial distribution. Collectively, these results indicated that p60 katanin‑like 1 is essential for oocyte meiosis by ensuring the integrity of the spindle poles.
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Copy and paste a formatted citation
Spandidos Publications style
Gao LL, Xu F, Jin Z, Ying XY and Liu JW: Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis. Mol Med Rep 20: 3573-3582, 2019.
APA
Gao, L., Xu, F., Jin, Z., Ying, X., & Liu, J. (2019). Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis. Molecular Medicine Reports, 20, 3573-3582. https://doi.org/10.3892/mmr.2019.10605
MLA
Gao, L., Xu, F., Jin, Z., Ying, X., Liu, J."Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis". Molecular Medicine Reports 20.4 (2019): 3573-3582.
Chicago
Gao, L., Xu, F., Jin, Z., Ying, X., Liu, J."Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis". Molecular Medicine Reports 20, no. 4 (2019): 3573-3582. https://doi.org/10.3892/mmr.2019.10605
Copy and paste a formatted citation
x
Spandidos Publications style
Gao LL, Xu F, Jin Z, Ying XY and Liu JW: Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis. Mol Med Rep 20: 3573-3582, 2019.
APA
Gao, L., Xu, F., Jin, Z., Ying, X., & Liu, J. (2019). Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis. Molecular Medicine Reports, 20, 3573-3582. https://doi.org/10.3892/mmr.2019.10605
MLA
Gao, L., Xu, F., Jin, Z., Ying, X., Liu, J."Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis". Molecular Medicine Reports 20.4 (2019): 3573-3582.
Chicago
Gao, L., Xu, F., Jin, Z., Ying, X., Liu, J."Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis". Molecular Medicine Reports 20, no. 4 (2019): 3573-3582. https://doi.org/10.3892/mmr.2019.10605
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