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miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2

  • Authors:
    • Yongquan Huang
    • Qiuke Hou
    • Haitao Su
    • Dongfeng Chen
    • Yiwen Luo
    • Tao Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Anatomy, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of Trauma, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510360, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3746-3754
    |
    Published online on: August 23, 2019
       https://doi.org/10.3892/mmr.2019.10613
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Abstract

It has been previously reported that psoralen, one of the active ingredients in Psoralea corylifolia, could induce osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), suggesting its potential to treat osteoporosis. Additionally, runt‑related transcription factor 2 (Runx2) is a transcription factor that plays vital roles in BMSC osteogenic differentiation. However, whether and how microRNAs (miRNAs/miRs) modulate osteogenic differentiation induced by psoralen have not yet been examined, to the best of the authors' knowledge. The present study aimed to identify the miRNA target genes that regulate osteogenic differentiation of BMSCs induced by psoralen. A Cell Counting Kit‑8 assay and alizarin red staining were used to detect the viability and osteogenic differentiation of BMSCs, respectively, under treatment with psoralen. miRNA microarray analysis was performed to identify the differentially expressed miRNAs under treatment with psoralen. A bioinformatics analysis and a luciferase reporter assay were conducted to identify the targets of miR‑488. Finally, the mechanisms of miR‑488 in psoralen‑induced BMSC osteogenic differentiation were investigated using overexpression or inhibition methods in vitro. Cell viability was elevated and osteogenic differentiation of BMSCs was improved under treatment with psoralen. miRNA microarray analysis and further validation by reverse transcription‑quantitative PCR revealed that miR‑488 was downregulated during psoralen‑induced BMSC osteogenic differentiation. Bioinformatics analysis and experimental validation by a luciferase reporter assay identified Runx2 as a potential target of miR‑488. Overexpression of miR‑488 by transfection with miR‑488 mimics markedly inhibited the expression of Runx2, Osterix and alkaline phosphatase, whereas, the inhibition of miR‑488 expression by the miR‑488 inhibitor promoted their expression compared with the control. Rescue assays demonstrated that Runx2 overexpression partially rescued the inhibitory effect of miR‑488 on BMSC osteogenic differentiation. The present results suggested that miR‑488 is a negative regulator of psoralen‑induced BMSC osteogenic differentiation by targeting Runx2, providing a possible therapeutic target for osteoporosis.
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View References

1 

Collison J: Osteoporosis: Teriparatide preferable for fracture prevention. Nat Rev Rheumatol. 14:42018. View Article : Google Scholar

2 

Chrischilles EA, Butler CD, Davis CS and Wallace RB: A model of lifetime osteoporosis impact. Arch Intern Med. 151:2026–2032. 1991. View Article : Google Scholar : PubMed/NCBI

3 

Pepe J, Cipriani C, Cecchetti V, et al: Patients' reasons for adhering to long-term alendronate therapy. Osteoporosis international. 2019 May 14;[Epub ahead of print] doi: 10.1007/s00198-019-05010-w. View Article : Google Scholar

4 

Wang C, Wang J, Li J, Hu G, Shan S, Li Q and Zhang X: KDM5A controls bone morphogenic protein 2-induced osteogenic differentiation of bone mesenchymal stem cells during osteoporosis. Cell Death Dis. 7:e23352016. View Article : Google Scholar : PubMed/NCBI

5 

Elango J, Robinson J, Zhang J, Bao B, Ma N, de Val JEMS and Wu W: Collagen peptide upregulates osteoblastogenesis from bone marrow mesenchymal stem cells through MAPK-Runx2. Cells. 8:2019. View Article : Google Scholar : PubMed/NCBI

6 

Qi M, Zhang L, Ma Y, Shuai Y, Li L, Luo K, Liu W and Jin Y: Autophagy maintains the function of bone marrow mesenchymal stem cells to prevent estrogen deficiency-induced osteoporosis. Theranostics. 7:4498–4516. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Jing H, Liao L, An Y, Su X, Liu S, Shuai Y, Zhang X and Jin Y: Suppression of EZH2 prevents the shift of osteoporotic MSC fate to adipocyte and enhances bone formation during osteoporosis. Mol Ther. 24:217–229. 2016. View Article : Google Scholar : PubMed/NCBI

8 

An J, Yang H, Zhang Q, Liu C, Zhao J, Zhang L and Chen B: Natural products for treatment of osteoporosis: The effects and mechanisms on promoting osteoblast-mediated bone formation. Life Sci. 147:46–58. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Tang DZ, Yang F, Yang Z, Huang J, Shi Q, Chen D and Wang YJ: Psoralen stimulates osteoblast differentiation through activation of BMP signaling. Biochem Biophys Res Commun. 405:256–261. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Czech MP: MicroRNAs as therapeutic targets. N Engl J Med. 354:1194–1195. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Laxman N, Mallmin H, Nilsson O and Kindmark A: miR-203 and miR-320 regulate bone morphogenetic protein-2-induced osteoblast differentiation by targeting distal-less homeobox 5 (Dlx5). Genes (Basel). 8:2016. View Article : Google Scholar : PubMed/NCBI

12 

Chao C, Li F, Tan Z, Zhang W, Yang Y and Luo C: miR-195 inhibited abnormal activation of osteoblast differentiation in MC3T3-E1 cells via targeting RAF-1. Exp Cell Res. 362:293–301. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Tian Z, Zhou H, Xu Y and Bai J: MicroRNA-495 inhibits new bone regeneration via targeting high mobility group AT-Hook 2 (HMGA2). Med Sci Monit. 23:4689–4698. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Almalki SG and Agrawal DK: Key transcription factors in the differentiation of mesenchymal stem cells. Differentiation. 92:41–51. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Stephens AS and Morrison NA: Novel target genes of RUNX2 transcription factor and 1,25-dihydroxyvitamin D3. J Cell Biochem. 115:1594–1608. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Meister G: Argonaute proteins: Functional insights and emerging roles. Nat Rev Genet. 14:447–459. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Hu D, Shen D, Zhang M, Jiang N, Sun F, Yuan S and Wan K: MiR-488 suppresses cell proliferation and invasion by targeting ADAM9 and lncRNA HULC in hepatocellular carcinoma. Am J Cancer Res. 7:2070–2080. 2017.PubMed/NCBI

19 

Yang Z, Feng Z, Gu J, Li X, Dong Q, Liu K, Li Y and OuYang L: microRNA-488 inhibits chemoresistance of ovarian cancer cells by targeting Six1 and mitochondrial function. Oncotarget. 8:80981–80993. 2017.PubMed/NCBI

20 

Lv Y, Shi Y, Han Q and Dai G: Histone demethylase PHF8 accelerates the progression of colorectal cancer and can be regulated by miR-488 in vitro. Mol Med Rep. 16:4437–4444. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Yu S, Geng Q, Pan Q, Liu Z, Ding S, Xiang Q, Sun F, Wang C, Huang Y and Hong A: MiR-690, a Runx2-targeted miRNA, regulates osteogenic differentiation of C2C12 myogenic progenitor cells by targeting NF-kappaB p65. Cell Biosci. 6:102016. View Article : Google Scholar : PubMed/NCBI

22 

Yu S, Geng Q, Ma J, Sun F, Yu Y, Pan Q and Hong A: Heparin-binding EGF-like growth factor and miR-1192 exert opposite effect on Runx2-induced osteogenic differentiation. Cell Death Dis. 4:e8682013. View Article : Google Scholar : PubMed/NCBI

23 

Li Z, Wang W, Xu H, Ning Y, Fang W, Liao W, Zou J, Yang Y and Shao N: Effects of altered CXCL12/CXCR4 axis on BMP2/Smad/Runx2/Osterix axis and osteogenic gene expressions during osteogenic differentiation of MSCs. Am J Transl Res. 9:1680–1693. 2017.PubMed/NCBI

24 

Kim HK, Cho SG, Kim JH, Doan TK, Hu QS, Ulhaq R, Song EK and Yoon TR: Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation. Life Sci. 84:290–295. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang Y, Hou Q, Su H, Chen D, Luo Y and Jiang T: miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2. Mol Med Rep 20: 3746-3754, 2019.
APA
Huang, Y., Hou, Q., Su, H., Chen, D., Luo, Y., & Jiang, T. (2019). miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2. Molecular Medicine Reports, 20, 3746-3754. https://doi.org/10.3892/mmr.2019.10613
MLA
Huang, Y., Hou, Q., Su, H., Chen, D., Luo, Y., Jiang, T."miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2". Molecular Medicine Reports 20.4 (2019): 3746-3754.
Chicago
Huang, Y., Hou, Q., Su, H., Chen, D., Luo, Y., Jiang, T."miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2". Molecular Medicine Reports 20, no. 4 (2019): 3746-3754. https://doi.org/10.3892/mmr.2019.10613
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Hou Q, Su H, Chen D, Luo Y and Jiang T: miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2. Mol Med Rep 20: 3746-3754, 2019.
APA
Huang, Y., Hou, Q., Su, H., Chen, D., Luo, Y., & Jiang, T. (2019). miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2. Molecular Medicine Reports, 20, 3746-3754. https://doi.org/10.3892/mmr.2019.10613
MLA
Huang, Y., Hou, Q., Su, H., Chen, D., Luo, Y., Jiang, T."miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2". Molecular Medicine Reports 20.4 (2019): 3746-3754.
Chicago
Huang, Y., Hou, Q., Su, H., Chen, D., Luo, Y., Jiang, T."miR‑488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2". Molecular Medicine Reports 20, no. 4 (2019): 3746-3754. https://doi.org/10.3892/mmr.2019.10613
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