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Article

MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer

  • Authors:
    • Li Fan
    • Yao Wang
    • Wei Huo
    • Wei‑Hua Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 3755-3763
    |
    Published online on: September 5, 2019
       https://doi.org/10.3892/mmr.2019.10650
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Abstract

MicroRNAs (miRNAs) are known to serve a role in tumorigenic programs. The dysregulated expression of miR‑301a‑3p may affect the progression of various types of human cancer; however, the expression and the role of miR‑301a‑3p in prostate cancer are still unclear. The present study aimed to clarify the role and molecular mechanism of miR‑301a‑3p in prostate cancer. The results demonstrated that the expression of miR‑301a‑3p was significantly upregulated in human prostate cancer tissues and in several prostate cancer cell lines. In vitro overexpression of miR‑301a‑3p notably increased prostate cancer cell proliferation and invasion. Bioinformatics analysis revealed that runt‑related transcription factor 3 (RUNX3) may be a target of miR‑301a‑3p, which was confirmed by Dual‑luciferase reporter assay. Western blot analysis also demonstrated that miR‑301a‑3p regulated the protein expression levels of RUNX3. In addition, the results indicated that miR‑301a‑3p may regulate the Wnt signaling pathway, and rescue experiments indicated that RUNX3 contributed to the effects of miR‑301a‑3p on cell proliferation and invasion through Wnt signaling. In conclusion, these findings suggested that miR‑301a‑3p may promote prostate cancer cell invasion and proliferation by targeting RUNX3, and provided insight into understanding prostate cancer pathogenesis. miR‑301a‑3p may be a potential therapeutic candidate to treat prostate cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fan L, Wang Y, Huo W and Wang WH: MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer. Mol Med Rep 20: 3755-3763, 2019.
APA
Fan, L., Wang, Y., Huo, W., & Wang, W. (2019). MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer. Molecular Medicine Reports, 20, 3755-3763. https://doi.org/10.3892/mmr.2019.10650
MLA
Fan, L., Wang, Y., Huo, W., Wang, W."MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer". Molecular Medicine Reports 20.4 (2019): 3755-3763.
Chicago
Fan, L., Wang, Y., Huo, W., Wang, W."MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer". Molecular Medicine Reports 20, no. 4 (2019): 3755-3763. https://doi.org/10.3892/mmr.2019.10650
Copy and paste a formatted citation
x
Spandidos Publications style
Fan L, Wang Y, Huo W and Wang WH: MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer. Mol Med Rep 20: 3755-3763, 2019.
APA
Fan, L., Wang, Y., Huo, W., & Wang, W. (2019). MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer. Molecular Medicine Reports, 20, 3755-3763. https://doi.org/10.3892/mmr.2019.10650
MLA
Fan, L., Wang, Y., Huo, W., Wang, W."MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer". Molecular Medicine Reports 20.4 (2019): 3755-3763.
Chicago
Fan, L., Wang, Y., Huo, W., Wang, W."MicroRNA‑301a‑3p overexpression promotes cell invasion and proliferation by targeting runt‑related transcription factor 3 in prostate cancer". Molecular Medicine Reports 20, no. 4 (2019): 3755-3763. https://doi.org/10.3892/mmr.2019.10650
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