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Article

MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ

  • Authors:
    • Huining Wang
    • Yuqin Shen
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Periodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 4271-4276
    |
    Published online on: September 12, 2019
       https://doi.org/10.3892/mmr.2019.10676
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Abstract

The present study aimed to explore the mechanisms through which microRNA (miR)‑20a may be involved in the differentiation of THP‑1 human acute monocytic leukemia cells into osteoclasts. THP‑1 cells were differentiated into macrophages (osteoclast precursors) and subsequently into osteoclast cells. The expression levels of miR‑20a in THP‑1 cells were significantly reduced in a time‑dependent manner during phorbol‑12‑myristate‑13‑acetate (PMA), macrophage colony‑stimulating factor (M‑CSF) and receptor activator of nuclear factor‑κB ligand RANKL‑induced osteoclastogenesis. Following transfection with a miR‑20a mimics, the levels of miR‑20a in PMA‑treated THP‑1 cells increased more than 40‑fold as compared with expression in the control cells. In addition, the overexpression of miR‑20a inhibited proliferation, initiated S phase cell cycle arrest and induced apoptosis of PMA‑treated THP‑1 cells. Additionally, miR‑20a mimics treatment notably decreased the levels of tartrate‑resistant acid phosphatase, nuclear factor of activated T‑cells, cytoplasmic 1 and peroxisome proliferator‑activated receptor γ (PPARγ) during THP‑1 cell further differentiation progress. In summary, miR‑20a may negatively regulate the proliferation and osteoclastogenesis of THP‑1 cells during its osteoclast differentiation progress by downregulating PPARγ.
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1 

Cheng J, Liang H, Li Q, Peng C, Li Z, Shi S, Yang L, Tian Z, Tian Y, Zhang Z and Cao W: Hematoporphyrin monomethyl ether-mediated photodynamic effects on THP-1 cell-derived macrophages. J Photochem Photobiol B. 101:9–15. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Sengupta S, Park SH, Seok GE, Patel A, Numata K, Lu CL and Kaplan DL: Quantifying osteogenic cell degradation of silk biomaterials. Biomacromolecules. 11:3592–3599. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Geijtenbeek TB, Torensma R, van Vliet SJ, van Duijnhoven GC, Adema GJ, van Kooyk Y and Figdor CG: Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 100:575–585. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Jakob F, Siggelkow H, Homann D, Köhrle J, Adamski J and Schütze N: Local estradiol metabolism in osteoblast- and osteoclast-like cells. J Steroid Biochem Mol Biol. 61:167–174. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Hattersley G, Owens J, Flanagan AM and Chambers TJ: Macrophage colony stimulating factor (M-CSF) is essential for osteoclast formation in vitro. Biochem Biophys Res Commun. 177:526–531. 1991. View Article : Google Scholar : PubMed/NCBI

6 

Takahashi N, Udagawa N and Suda T: A new member of tumor necrosis factor ligand family, ODF/OPGL/TRANCE/RANKL, regulates osteoclast differentiation and function. Biochem Biophys Res Commun. 256:449–455. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, et al: Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 3:889–901. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Matsumoto M, Kogawa M, Wada S, Takayanagi H, Tsujimoto M, Katayama S, Hisatake K and Nogi Y: Essential role of p38 mitogen-activated protein kinase in cathepsin K gene expression during osteoclastogenesis through association of NFATc1 and PU.1. J Biol Chem. 279:45969–45979. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Qu B, Xia X, Yan M, Gong K, Deng S, Huang G, Ma Z and Pan X: miR-218 is involved in the negative regulation of osteoclastogenesis and bone resorption by partial suppression of p38MAPK-c-Fos-NFATc1 signaling: Potential role for osteopenic diseases. Exp Cell Res. 338:89–96. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Kapinas K and Delany AM: MicroRNA biogenesis and regulation of bone remodeling. Arthritis Res Ther. 13:2202011. View Article : Google Scholar : PubMed/NCBI

12 

Fontana L, Pelosi E, Greco P, Racanicchi S, Testa U, Liuzzi F, Croce CM, Brunetti E, Grignani F and Peschle C: MicroRNAs 17-5p-20a-106a control monocytopoiesis through AML1 targeting and M-CSF receptor upregulation. Nat Cell Biol. 9:775–787. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Sun KT, Chen MY, Tu MG, Wang IK, Chang SS and Li CY: MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 73:145–153. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Shi C, Qi J, Huang P, Jiang M, Zhou Q, Zhou H, Kang H, Qian N, Yang Q, Guo L and Deng L: MicroRNA-17/20a inhibits glucocorticoid-induced osteoclast differentiation and function through targeting RANKL expression in osteoblast cells. Bone. 68:67–75. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Zhou BR, Guo XF, Zhang JA, Xu Y, Li W, Wu D, Yin ZQ, Permatasari F and Luo D: Elevated miR-34c-5p mediates dermal fibroblast senescence by ultraviolet irradiation. Int J Biol Sci. 9:743–752. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Kim MH, Ryu SY, Choi JS, Min YK and Kim SH: Saurolactam inhibits osteoclast differentiation and stimulates apoptosis of mature osteoclasts. J Cell Physiol. 221:618–628. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

M'Baya-Moutoula E, Louvet L, Metzinger-Le Meuth V, Massy ZA and Metzinger L: High inorganic phosphate concentration inhibits osteoclastogenesis by modulating miR-223. Biochim Biophys Acta. 1852:2202–2212. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Yuan FL, Xu RS, Jiang DL, He XL, Su Q, Jin C and Li X: Leonurine hydrochloride inhibits osteoclastogenesis and prevents osteoporosis associated with estrogen deficiency by inhibiting the NF-κB and PI3K/Akt signaling pathways. Bone. 75:128–137. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Wan Y, Chong LW and Evans RM: PPAR-gamma regulates osteoclastogenesis in mice. Nat Med. 13:1496–1503. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Li H, Li T, Fan J, Li T, Fan L, Wang S, Weng X, Han Q and Zhao RC: miR-216a rescues dexamethasone suppression of osteogenesis, promotes osteoblast differentiation and enhances bone formation, by regulating c-Cbl-mediated PI3K/AKT pathway. Cell Death Differ. 22:1935–1945. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Guo DW, Han YX, Cong L, Liang D and Tu GJ: Resveratrol prevents osteoporosis in ovariectomized rats by regulating microRNA-338-3p. Mol Med Report. 12:2098–2106. 2015. View Article : Google Scholar

23 

Sun T, Leung F and Lu WW: miR-9-5p, miR-675-5p and miR-138-5p damages the strontium and LRP5-mediated skeletal cell proliferation, differentiation, and adhesion. Int J Mol Sci. 17:2362016. View Article : Google Scholar : PubMed/NCBI

24 

Tiago DM, Marques CL, Roberto VP, Cancela ML and Laizé V: Mir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish. Arch Biochem Biophys. 543:23–30. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Zhang JF, Fu WM, He ML, Xie WD, Lv Q, Wan G, Li G, Wang H, Lu G, Hu X, et al: MiRNA-20a promotes osteogenic differentiation of human mesenchymal stem cells by co-regulating BMP signaling. RNA Biol. 8:829–838. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Zhou M, Ma J, Chen S, Chen X and Yu X: MicroRNA-17-92 cluster regulates osteoblast proliferation and differentiation. Endocrine. 45:302–310. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Zhou G, Chen T and Raj JU: MicroRNAs in pulmonary arterial hypertension. Am J Respir Cell Mol Biol. 52:139–151. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Krishnan A, Nair SA and Pillai MR: Biology of PPAR gamma in cancer: A critical review on existing lacunae. Curr Mol Med. 7:532–540. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Zhou J, Guo F, Wang G, Wang J, Zheng F, Guan X, Chang A, Zhang X, Dai C, Li S, et al: miR-20a regulates adipocyte differentiation by targeting lysine-specific demethylase 6b and transforming growth factor-β signaling. Int J Obes (Lond). 39:1282–1291. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Fan J, Li J and Fan Q: Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA-20a and downregulating the expression levels of PPARγ. Mol Med Report. 12:4759–4765. 2015. View Article : Google Scholar

31 

Shao B, Liao L, Yu Y, Shuai Y, Su X, Jing H, Yang D and Jin Y: Estrogen preserves Fas ligand levels by inhibiting microRNA-181a in bone marrow-derived mesenchymal stem cells to maintain bone remodeling balance. FASEB J. 29:3935–3944. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Fan MQ, Huang CB, Gu Y, Xiao Y, Sheng JX and Zhong L: Decrease expression of microRNA-20a promotes cancer cell proliferation and predicts poor survival of hepatocellular carcinoma. J Exp Clin Canc Res. 32:212013. View Article : Google Scholar

33 

Trompeter HI, Abbad H, Iwaniuk KM, Hafner M, Renwick N, Tuschl T, Schira J, Müller HW and Wernet P: MicroRNAs MiR-17, MiR-20a, and MiR-106b act in concert to modulate E2F activity on cell cycle arrest during neuronal lineage differentiation of USSC. PLoS One. 6:e161382011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang H and Shen Y: MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ. Mol Med Rep 20: 4271-4276, 2019.
APA
Wang, H., & Shen, Y. (2019). MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ. Molecular Medicine Reports, 20, 4271-4276. https://doi.org/10.3892/mmr.2019.10676
MLA
Wang, H., Shen, Y."MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ". Molecular Medicine Reports 20.5 (2019): 4271-4276.
Chicago
Wang, H., Shen, Y."MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ". Molecular Medicine Reports 20, no. 5 (2019): 4271-4276. https://doi.org/10.3892/mmr.2019.10676
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H and Shen Y: MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ. Mol Med Rep 20: 4271-4276, 2019.
APA
Wang, H., & Shen, Y. (2019). MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ. Molecular Medicine Reports, 20, 4271-4276. https://doi.org/10.3892/mmr.2019.10676
MLA
Wang, H., Shen, Y."MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ". Molecular Medicine Reports 20.5 (2019): 4271-4276.
Chicago
Wang, H., Shen, Y."MicroRNA‑20a negatively regulates the growth and osteoclastogenesis of THP‑1 cells by downregulating PPARγ". Molecular Medicine Reports 20, no. 5 (2019): 4271-4276. https://doi.org/10.3892/mmr.2019.10676
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