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Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells

  • Authors:
    • Haibo Yu
    • Wenyi Yu
    • Ying Liu
    • Xiao Yuan
    • Rongtao Yuan
    • Qingyuan Guo
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The Affiliated Qingdao Municipal Hospital, Qingdao University, Qingdao, Shandong 266011, P.R. China, Department of Orthodontics II, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266011, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4489-4498
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    Published online on: September 30, 2019
       https://doi.org/10.3892/mmr.2019.10715
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Abstract

During orthodontic treatment, mechanical force is applied to the teeth, and following a series of complex metabolism changes, the position of the teeth in the alveolar bone change. This process is closely associated with primitive bone mesenchymal stem cells (BMSCs), which may differentiate into osteoblasts precursor cell. A hypoxic microenvironment may be caused by orthodontic mechanical forces between the alveolar bone and the root. Hypoxia‑inducible factor 1α (HIF‑1α) is a specific receptor that adapts to a hypoxic environment. The present study was designed to investigate whether HIF‑1α was involved in the osteoblastic differentiation of BMSCs induced by cyclic tensile stress. During this process, HIF‑1α mRNA and protein expression were detected using a reverse transcription‑quantitative polymerase chain reaction and western blotting. It was revealed that alkaline phosphatase activity increased in a time‑dependent manner in three different stretching strength groups, which indicates that cyclic stretch promotes the osteogenic differentiation of BMSCs. The optimal force stage of osteogenesis was an unexpected discovery, which will provide theoretical guidance for selecting the most suitable orthodontic force for tooth movement in clinical orthodontic treatment. Most importantly, all experiments revealed that HIF‑1α mRNA and protein were significantly increased following stretching treatment in BMSCs. It was therefore concluded that HIF‑1α may be involved in BMSCs modulating osteogenic metabolism during exposure to cyclic stretch and a hypoxic microenvironment, which may prove useful for the reconstruction of a jaw during orthodontic treatment.
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1 

Dibart S, Yee C, Surmenian J, Sebaoun JD, Baloul S, Goguet-Surmenian E and Kantarci A: Tissue response during Piezocision-assisted tooth movement: A histological study in rats. Eur J Orthod. 36:457–464. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Antoun JS, Mei L, Gibbs K and Farella M: Effect of orthodontic treatment on the periodontal tissues. Periodontol. 74:140–157. 2017. View Article : Google Scholar

3 

Vlaikou AM, Kouroupis D, Sgourou A, Markopoulos GS, Bagli E, Markou M, Papadopoulou Z, Fotsis T, Nakos G, Lekka ME and Syrrou M: Mechanical stress affects methylation pattern of GNAS isoforms and osteogenic differentiation of hAT-MSCs. Biochim Biophys Acta Mol Cell Res. 1864:1371–1381. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Barbieri G, Solano P, Alarcón JA, Vernal R, Rios-Lugo J, Sanz M and Martín C: Biochemical markers of bone metabolism in gingival crevicular fluid during early orthodontic tooth movement. Angle Orthod. 83:63–69. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Ohgushi H: Osteogenically differentiated mesenchymal stem cells and ceramics for bone tissue engineering. Expert Opin Biol Ther. 14:197–208. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Connelly JT, García AJ and Levenston ME: Interactions between integrin ligand density and cytoskeletal integrity regulate BMSC chondrogenesis. J Cell Physiol. 217:145–154. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Moreau JE, Chen J, Horan RL, Kaplan DL and Altman GH: Sequential growth factor application in bone marrow stromal cell ligament engineering. Tissue Eng. 11:1887–1897. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Moreau JE, Bramono DS, Horan RL, Kaplan DL and Altman GH: Sequential biochemical and mechanical stimulation in the development of tissue-engineered ligaments. Tissue Eng Part A. 14:1161–1172. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Schaffer JL, Rizen M, L'Italien GJ, Benbrahim A, Megerman J, Gerstenfeld LC and Gray ML: Device for the application of a dynamic biaxially uniform and Isotropic strain to a flexible cell culture membrane. J Orthop Res. 12:709–719. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Susilo ME, Bell BJ, Roeder BA, Voytik-Harbin SL, Kokini K and Nauman EA: Prediction of equibiaxial loading stress in collagen-based extracellular matrix using a three-dimensional unit cell model. Acta Biomater. 9:5544–5553. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Qi YX, Yao QP, Huang K, Shi Q, Zhang P, Wang GL, Han Y, Bao H, Wang L, Li HP, et al: Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application. Proc Natl Acad Sci USA. 113:5293–5298. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Ursekar CP, Teo SK, Hirata H, Harada I, Chiam KH and Sawada Y: Design and construction of an equibiaxial cell stretching system that is improved for biochemical analysis. PLoS One. 9:e906652014. View Article : Google Scholar : PubMed/NCBI

13 

Lohberger B, Kaltenegger H, Stuendl N, Payer M, Rinner B and Leithner A: Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells. Biomed Res Int. 2014:1895162014. View Article : Google Scholar : PubMed/NCBI

14 

Liu Y, Huang X, Yu H, Yang J, Li Y, Yuan X and Guo Q: HIF-1α-TWIST pathway restrains cyclic mechanical stretch-induced osteogenic differentiation of bone marrow mesenchymal stem cells. Connect Tissue Res. 60:544–554. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Niklas A, Proff P, Gosau M and Römer P: The role of hypoxia in orthodontic tooth movement. Int J Dent. 2013:8418402013. View Article : Google Scholar : PubMed/NCBI

16 

Semenza GL and Wang GL: A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation. Mol Cell Biol. 12:5447–5454. 1992. View Article : Google Scholar : PubMed/NCBI

17 

Li Y, Padmanabha D, Gentile LB, Dumur CI, Beckstead RB and Baker KD: HIF- and non-HIF-regulated hypoxic responses require the estrogen-related receptor in Drosophila melanogaster. PLoS Genet. 9:e10032302013. View Article : Google Scholar : PubMed/NCBI

18 

Zhou N, Hu N, Liao JY, Lin LB, Zhao C, Si WK, Yang Z, Yi SX, Fan TX, Bao W, et al: HIF-1a as a regulator of BMP2-induced chondrogenic differentiation, osteogenic differentiation, and endochondral ossification in stem cells. Cell Physiol Biochem. 36:44–60. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Nemeth K, Mayer B, Sworder BJ, Kuznetsov SA and Mezey E: A practical guide to culturing mouse and human bone marrow stromal cells. Curr Protoc Immunol. 102:Unit 22F.12. 2013.PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Yu HC, Wu TC, Chen MR, Liu SW, Chen JH and Lin KM: Mechanical stretching induces osteoprotegerin in differentiating C2C12 precursor cells through noncanonical Wnt pathways. J Bone Miner Res. 25:1128–1137. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Jang Y, Han J, Kim SJ, Kim J, Lee MJ, Jeong S, Ryu MJ, Seo KS, Choi SY, Shong M, et al: Suppression of mitochondrial respiration with auraptene inhibits the progression of renal cell carcinoma: Involvement of HIF-1a degradation. Oncotarget. 6:38127–38138. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Sung WW, Chu YC, Chen PR, Liao MH and Lee JW: Positive regulation of HIF-1A expression by EBV oncoprotein LMP1 in nasopharyngeal carcinoma cells. Cancer Lett. 382:21–31. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Sha Y, Lv Y, Xu Z, Yang L, Hao X and Afandi R: MGF E peptide pretreatment improves the proliferation and osteogenic differentiation of BMSCs via MEK-ERK1/2 and PI3K-Akt pathway under severe hypoxia. Life Sci. 189:52–62. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Bruder SP, Jaiswal N and Haynesworth SE: Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem. 64:278–294. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Kusuyama J, Kamisono A, ChangHwan S, Amir MS, Bandow K, Eiraku N, Ohnishi T and Matsuguchi T: Spleen tyrosine kinase influences the early stages of multilineage differentiation of bone marrow stromal cell lines by regulating phospholipase C gamma activities. J Cell Physiol. 233:2549–2559. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Tang Y, Xu Y, Xiao Z, Zhao Y, Li J, Han S, Chen L, Dai B, Wang L, Chen B and Wang H: The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs. Sci Rep. 7:1922017. View Article : Google Scholar : PubMed/NCBI

28 

Xia CS, Zuo AJ, Wang CY and Wang YZ: Isolation of rabbit bone marrow mesenchymal stem cells using density gradient centrifugation and adherence screening methods. Minerva Med. 104:519–525. 2013.PubMed/NCBI

29 

Steinert AF, Kunz M, Prager P, Göbel S, Klein-Hitpass L, Ebert R, Nöth U, Jakob F and Gohlke F: Characterization of bursa subacromialis-derived mesenchymal stem cells. Stem Cell Res Ther. 6:1142015. View Article : Google Scholar : PubMed/NCBI

30 

Jiang F, Xia Z, Li S, Eckert G and Chen J: Mechanical environment change in root, periodontal ligament, and alveolar bone in response to two canine retraction treatment strategies. Orthod Craniofac Res. 18 (Suppl 1):S29–S38. 2015. View Article : Google Scholar

31 

Stanley A, Heo SJ, Mauck RL, Mourkioti F and Shore EM: Elevated BMP and mechanical signaling through YAP1/RhoA poises FOP mesenchymal progenitors for osteogenesis. J Bone Miner Res. May 20–2019.doi: 10.1002/jbmr.3760 (Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

32 

Estes BT, Gimble JM and Guilak F: Mechanical signals as regulators of stem cell fate. Curr Top Dev Biol. 60:91–126. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Simmons CA, Matlis S, Thornton AJ, Chen S, Wang CY and Mooney DJ: Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway. J Biomech. 36:1087–1096. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Jagodzinski M, Drescher M, Zeichen J, Hankemeier S, Krettek C, Bosch U and van Griensven M: Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells. Eur Cell Mater. 7:35–41. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Boccafoschi F, Sabbatini M, Bosetti M and Cannas M: Overstressed mechanical stretching activates survival and apoptotic signals in fibroblasts. Cells Tissues Organs. 192:167–176. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Parandakh A, Tafazzoli-Shadpour M and Khani MM: Stepwise morphological changes and cytoskeletal reorganization of human mesenchymal stem cells treated by short-time cyclic uniaxial stretch. In Vitro Cell Dev Biol Anim. 53:547–553. 2017. View Article : Google Scholar : PubMed/NCBI

37 

Kajiya H, Katsumata Y, Sasaki M, Tsutsumi T, Kawaguchi M and Fukushima T: Photothermal stress triggered by near-infrared-irradiated carbon nanotubes up-regulates osteogenesis and mineral deposition in tooth-extracted sockets. Int J Hyperthermia. 31:635–642. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Friedenstein AJ: Precursor cells of mechanocytes. Int Rev Cytol. 47:327–359. 1976. View Article : Google Scholar : PubMed/NCBI

39 

Han I, Kwon BS, Park HK and Kim KS: Differentiation potential of mesenchymal stem cells is related to their intrinsic mechanical properties. Int Neurourol J. 21 (Suppl 1):S24–S31. 2017. View Article : Google Scholar : PubMed/NCBI

40 

Huang J, Deng F, Wang L, Xiang XR, Zhou WW, Hu N and Xu L: Hypoxia induces osteogenesis-related activities and expression of core binding factor alpha1 in mesenchymal stem cells. Tohoku J Exp Med. 224:7–12. 2011. View Article : Google Scholar : PubMed/NCBI

41 

Balamurugan K: HIF-1 at the crossroads of hypoxia, inflammation, and cancer. Int J Cancer. 138:1058–1066. 2016. View Article : Google Scholar : PubMed/NCBI

42 

Shah YM: The role of hypoxia in intestinal inflammation. Mol Cell Pediatr. 3:12016. View Article : Google Scholar : PubMed/NCBI

43 

Zhu L and Zhao Q: Hypoxia-inducible factor 1a participates in hypoxia-induced epithelial-mesenchymal transition via response gene to complement 32. Exp Ther Med. 14:1825–1831. 2017. View Article : Google Scholar : PubMed/NCBI

44 

De Luna N, Suárez-Calvet X, Lleixà C, Diaz-Manera J, Olivé M, Illa I and Gallardo E: Hypoxia triggers IFN-I production in muscle: Implications in dermatomyositis. Sci Rep. 7:85952017. View Article : Google Scholar : PubMed/NCBI

45 

Moirangthem RD, Singh S, Adsul A, Jalnapurkar S, Limaye L and Kale VP: Hypoxic niche-mediated regeneration of hematopoiesis in the engraftment window is dominantly affected by oxygen tension in the milieu. Stem Cells Dev. 24:2423–2436. 2015. View Article : Google Scholar : PubMed/NCBI

46 

Jiang Y, Wang Y and Tang G: Cyclic tensile strain promotes the osteogenic differentiation of a bone marrow stromal cell and vascular endothelial cell co-culture system. Arch Biochem Biophys. 607:37–43. 2016. View Article : Google Scholar : PubMed/NCBI

47 

Hsu SH, Chen CT and Wei YH: Inhibitory effects of hypoxia on metabolic switch and osteogenic differentiation of human mesenchymal stem cells. Stem Cells. 31:2779–2788. 2013. View Article : Google Scholar : PubMed/NCBI

48 

Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D and Wang C: Expression and function of hypoxia inducible factor-1α and vascular endothelial growth factor in pulp tissue of teeth under orthodontic movement. Mediators Inflamm. 2015:2157612015. View Article : Google Scholar : PubMed/NCBI

49 

Wang Y, Wan C, Gilbert SR and Clemens TL: Oxygen sensing and osteogenesis. Ann N Y Acad Sci. 1117:1–11. 2007. View Article : Google Scholar : PubMed/NCBI

50 

Wan C, Shao J, Gilbert SR, Riddle RC, Long F, Johnson RS, Schipani E and Clemens TL: Role of HIF-1alpha in skeletal development. Ann N Y Acad Sci. 1192:322–326. 2010. View Article : Google Scholar : PubMed/NCBI

51 

Menon A, Creo P, Piccoli M, Bergante S, Conforti E, Banfi G, Randelli P and Anastasia L: Chemical activation of the hypoxia-inducible factor reversibly reduces tendon stem cell proliferation, inhibits their differentiation, and maintains cell undifferentiation. Stem Cells Int. 2018:94680852018. View Article : Google Scholar : PubMed/NCBI

52 

Lampert FM, Kütscher C, Stark GB and Finkenzeller G: Overexpression of Hif-1a in mesenchymal stem cells affects cell-autonomous angiogenic and osteogenic parameters. J Cell Biochem. 117:760–768. 2016. View Article : Google Scholar : PubMed/NCBI

53 

Yang DC, Yang MH, Tsai CC, Huang TF, Chen YH and Hung SC: Hypoxia inhibits osteogenesis in human mesenchymal stem cells through direct regulation of RUNX2 by TWIST. PLoS One. 6:e239652011. View Article : Google Scholar : PubMed/NCBI

54 

Wagegg M, Gaber T, Lohanatha FL, Hahne M, Strehl C, Fangradt M, Tran CL, Schönbeck K, Hoff P, Ode A, et al: Hypoxia promotes osteogenesis but suppresses adipogenesis of human mesenchymal stromal cells in a hypoxia-inducible factor-1 dependent manner. PLoS One. 7:e464832012. View Article : Google Scholar : PubMed/NCBI

55 

Chen X, Gu S, Chen BF, Shen WL, Yin Z, Xu GW, Hu JJ, Zhu T, Li G, Wan C, et al: Nanoparticle delivery of stable miR-199a-5p agomir improves the osteogenesis of human mesenchymal stem cells via the HIF1a pathway. Biomaterials. 53:239–250. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu H, Yu W, Liu Y, Yuan X, Yuan R and Guo Q: Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells. Mol Med Rep 20: 4489-4498, 2019.
APA
Yu, H., Yu, W., Liu, Y., Yuan, X., Yuan, R., & Guo, Q. (2019). Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells. Molecular Medicine Reports, 20, 4489-4498. https://doi.org/10.3892/mmr.2019.10715
MLA
Yu, H., Yu, W., Liu, Y., Yuan, X., Yuan, R., Guo, Q."Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells". Molecular Medicine Reports 20.5 (2019): 4489-4498.
Chicago
Yu, H., Yu, W., Liu, Y., Yuan, X., Yuan, R., Guo, Q."Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells". Molecular Medicine Reports 20, no. 5 (2019): 4489-4498. https://doi.org/10.3892/mmr.2019.10715
Copy and paste a formatted citation
x
Spandidos Publications style
Yu H, Yu W, Liu Y, Yuan X, Yuan R and Guo Q: Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells. Mol Med Rep 20: 4489-4498, 2019.
APA
Yu, H., Yu, W., Liu, Y., Yuan, X., Yuan, R., & Guo, Q. (2019). Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells. Molecular Medicine Reports, 20, 4489-4498. https://doi.org/10.3892/mmr.2019.10715
MLA
Yu, H., Yu, W., Liu, Y., Yuan, X., Yuan, R., Guo, Q."Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells". Molecular Medicine Reports 20.5 (2019): 4489-4498.
Chicago
Yu, H., Yu, W., Liu, Y., Yuan, X., Yuan, R., Guo, Q."Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells". Molecular Medicine Reports 20, no. 5 (2019): 4489-4498. https://doi.org/10.3892/mmr.2019.10715
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