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Article

Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration

  • Authors:
    • Chung‑Hsin Yeh
    • Li‑Wei Sun
    • Chang‑Mei Lai
    • Tzu‑Pei Yeh
    • Jong‑Ni Lin
    • Shiow‑Luan Tsay
    • Chin‑Chu Chen
    • Wan‑Ping Chen
    • Chien‑Min Chen
    • Ru‑Yin Tsai
  • View Affiliations / Copyright

    Affiliations: College of Nursing and Health Sciences, Da‑Yeh University, Changhua 51591, Taiwan, R.O.C., Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung 40201, Taiwan, R.O.C., Department of Emergency Medicine, Asia University Hospital, Taichung 41354, Taiwan, R.O.C., School of Nursing, China Medical University, Taichung 40402, Taiwan, R.O.C., Grape King Biotechnology Inc., Taoyuan 32097, Taiwan, R.O.C.
  • Pages: 5279-5285
    |
    Published online on: October 15, 2019
       https://doi.org/10.3892/mmr.2019.10745
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Abstract

Microglia serve important roles in chronic pain signal transduction pathways. Glia cells, especially microglia, seem to share mechanisms that lead to chronic pain and morphine‑induced tolerance. Evidence has suggested that downregulating cytoskeleton activity in microglia provides pain relief in chronic pain and morphine tolerance. The purpose of the present study was to evaluate the effect of ethanol extracts of Hericium erinaceus (EHE) mycelium on morphine‑induced BV2 microglial cell activation. BV2 cells were starved for 4 h in DMEM before being incubated with 100 ng/ml EHE for 30 min, followed by 1 µM morphine for 2 h. Subsequently, the cells were harvested and used for migration experiments and western blotting. The results showed that 1 µM morphine enhanced BV2 cell activation and chemotactic reaction, and it increased histone deacetylase 6 (HDAC6) expression and heat shock protein 90 (HSP90) deacetylation as well as HSP90 cleavage. Pretreatment with 100 ng/ml EHE significantly inhibited the morphine‑stimulated effects on BV2 cells. The present study demonstrated that EHE inhibited morphine‑induced BV2 activations by regulating the HDAC6/HSP90 deacetylation signal transduction pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yeh CH, Sun LW, Lai CM, Yeh TP, Lin JN, Tsay SL, Chen CC, Chen WP, Chen CM, Tsai RY, Tsai RY, et al: Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration. Mol Med Rep 20: 5279-5285, 2019.
APA
Yeh, C., Sun, L., Lai, C., Yeh, T., Lin, J., Tsay, S. ... Tsai, R. (2019). Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration. Molecular Medicine Reports, 20, 5279-5285. https://doi.org/10.3892/mmr.2019.10745
MLA
Yeh, C., Sun, L., Lai, C., Yeh, T., Lin, J., Tsay, S., Chen, C., Chen, W., Chen, C., Tsai, R."Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration". Molecular Medicine Reports 20.6 (2019): 5279-5285.
Chicago
Yeh, C., Sun, L., Lai, C., Yeh, T., Lin, J., Tsay, S., Chen, C., Chen, W., Chen, C., Tsai, R."Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration". Molecular Medicine Reports 20, no. 6 (2019): 5279-5285. https://doi.org/10.3892/mmr.2019.10745
Copy and paste a formatted citation
x
Spandidos Publications style
Yeh CH, Sun LW, Lai CM, Yeh TP, Lin JN, Tsay SL, Chen CC, Chen WP, Chen CM, Tsai RY, Tsai RY, et al: Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration. Mol Med Rep 20: 5279-5285, 2019.
APA
Yeh, C., Sun, L., Lai, C., Yeh, T., Lin, J., Tsay, S. ... Tsai, R. (2019). Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration. Molecular Medicine Reports, 20, 5279-5285. https://doi.org/10.3892/mmr.2019.10745
MLA
Yeh, C., Sun, L., Lai, C., Yeh, T., Lin, J., Tsay, S., Chen, C., Chen, W., Chen, C., Tsai, R."Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration". Molecular Medicine Reports 20.6 (2019): 5279-5285.
Chicago
Yeh, C., Sun, L., Lai, C., Yeh, T., Lin, J., Tsay, S., Chen, C., Chen, W., Chen, C., Tsai, R."Effect of ethanol extracts of Hericium erinaceus mycelium on morphine‑induced microglial migration". Molecular Medicine Reports 20, no. 6 (2019): 5279-5285. https://doi.org/10.3892/mmr.2019.10745
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