Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2019 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2019 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation

  • Authors:
    • Minyi Yu
    • Zheng Guan
    • Shanwen Li
    • Xianli Wen
    • Huimin Shi
    • Gaoting Qu
    • Xiaoyu Lu
    • Xianyi Zhu
    • Bin Wang
    • Qihua Feng
    • Weihua Gan
    • Aiqing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, P.R. China, Institute of Nephrology, Zhong Da Hospital, Southeast University, Nanjing, Jiangsu 210000, P.R. China, Department of Rheumatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5297-5303
    |
    Published online on: October 16, 2019
       https://doi.org/10.3892/mmr.2019.10753
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hyperproliferation of mesangial cells (MCs) is the central pathological feature observed in certain human renal diseases. Furthermore, the long non‑coding RNA uc.412 is regulated by transforming growth factor β1 in mesangial cells in vitro. The present study aimed to investigate whether uc.412 serves a role in renal fibrosis and whether it may be considered as a therapeutic target in mesangial proliferative kidney diseases. The results demonstrated that uc.412 overexpression significantly increased MC proliferation. The transcriptional profile of MCs overexpressing uc.412 was assessed by RNA sequencing. A total of 462 up‑ and 843 downregulated genes were identified (|fold change| ≥1.5), and reverse transcription‑quantitative PCR was used to determine the expression of these differentially expressed genes (DEGs). Subsequently, the potential function of these DEGs was determined by bioinformatics analyses. The results indicated that these DEGs were involved in numerous signaling pathways associated with MC proliferation. The downstream association between up‑ and downregulated genes was constructed via the STRING database. The protein‑protein interaction network indicated that serpin family E member 1 and matrix metallopeptidase 3 may be hub proteins. In conclusion, the present study provided novel insight into the role of uc.412 in MC proliferation, which may aid in the development of novel treatment for mesangial proliferative kidney diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Cove-Smith A and Hendry BM: The regulation of mesangial cell proliferation. Nephron Exp Nephrol. 108:e74–e79. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Kurogi Y: Mesangial cell proliferation inhibitors for the treatment of proliferative glomerular disease. Med Res Rev. 23:15–31. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Zhang A, Han Y, Wang B, Li S and Gan W: Beyond gap Junction channel function: The expression of Cx43 contributes to aldosterone-induced mesangial cell proliferation via the ERK1/2 and PKC pathways. Cell Physiol Biochem. 36:1210–1222. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Loeffler I and Wolf G: Transforming growth factor-β and the progression of renal disease. Nephrol Dial Transplant. 29 (Suppl 1):i37–i45. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Rodrigues-Diez R, Rayego-Mateos S, Orejudo M, Aroeira LS, Selgas R, Ortiz A, Egido J and Ruiz-Ortega M: TGF-beta blockade increases renal inflammation caused by the C-terminal module of the CCN2. Mediators Inflamm. 2015:5060412015. View Article : Google Scholar : PubMed/NCBI

6 

Kopp F and Mendell JT: Functional classification and experimental dissection of long noncoding RNAs. Cell. 172:393–407. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Tang PM, Tang PC, Chung JY and Lan HY: TGF-β1 signaling in kidney disease: From Smads to long non-coding RNAs. Noncoding RNA Res. 2:68–73. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Tang W, Zhang D and Ma X: RNA-sequencing reveals genome-wide long non-coding RNAs profiling associated with early development of diabetic nephropathy. Oncotarget. 8:105832–105847. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Bai X, Geng J, Li X, Wan J, Liu J, Zhou Z and Liu X: Long noncoding RNA LINC01619 regulates MicroRNA-27a/Forkhead box protein O1 and endoplasmic reticulum stress-mediated podocyte injury in diabetic nephropathy. Antioxid Redox Signal. 29:355–376. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Qu L, Ding J, Chen C, Wu ZJ, Liu B, Gao Y, Chen W, Liu F, Sun W, Li XF, et al: Exosome-transmitted lncARSR promotes sunitinib resistance in renal cancer by acting as a competing endogenous RNA. Cancer Cell. 29:653–668. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Zhang A, He Y, Wang B, Shi H and Gan W: Analysis of the differential expression of long noncoding RNAs in experimental mesangial cells proliferation induced by TGF-β. Chin J Nephrol. 31:774–779. 2015.

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Ma F, Fuqua BK, Hasin Y, Yukhtman C, Vulpe CD, Lusis AJ and Pellegrini M: A comparison between whole transcript and 3′RNA sequencing methods using Kapa and Lexogen library preparation methods. BMC Genomics. 20:92019. View Article : Google Scholar : PubMed/NCBI

14 

Huang da W, Sherman BT and Lempicki RA: Bioinformatics enrichment tools: Paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res. 37:1–13. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Huang da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2008. View Article : Google Scholar

16 

Cui Y, Huang Y, Wu X, Zheng M, Xia Y, Fu Z, Ge H, Wang S and Xie H: Hypoxia-induced tRNA-derived fragments, novel regulatory factor for doxorubicin resistance in triple-negative breast cancer. J Cell Physiol. 234:8740–8751. 2019. View Article : Google Scholar : PubMed/NCBI

17 

Zhai W, Sun Y, Jiang M, Wang M, Gasiewicz TA, Zheng J and Chang C: Differential regulation of LncRNA-SARCC suppresses VHL-mutant RCC cell proliferation yet promotes VHL-normal RCC cell proliferation via modulating androgen receptor/HIF-2α/C-MYC axis under hypoxia. Oncogene. 35:4866–4880. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Dong T, Peng Y, Zhong N, Liu F, Zhang H, Xu M, Liu R, Han M, Tian X, Jia J, et al: Perfluorodecanoic acid (PFDA) promotes gastric cell proliferation via sPLA2-IIA. Oncotarget. 8:50911–50920. 2017.PubMed/NCBI

19 

Kawakami T, Ren S and Duffield JS: Wnt signalling in kidney diseases: Dual roles in renal injury and repair. J Pathol. 229:221–231. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Ying Q and Wu G: Molecular mechanisms involved in podocyte EMT and concomitant diabetic kidney diseases: An update. Ren Fail. 39:474–483. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Lan T, Wu T, Chen C, Chen X, Hao J, Huang J, Wang L and Huang H: Berberine attenuates high glucose-induced proliferation and extracellular matrix accumulation in mesangial cells: Involvement of suppression of cell cycle progression and NF-κB/AP-1 pathways. Mol Cell Endocrinol. 384:109–116. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Chen D, Li Y, Mei Y, Geng W, Yang J, Hong Q, Feng Z, Cai G, Zhu H, Shi S, et al: miR-34a regulates mesangial cell proliferation via the PDGFR-β/Ras-MAPK signaling pathway. Cell Mol Life Sci. 71:4027–4042. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Zhou X, Workeneh B, Hu Z and Li R: Effect of immunosuppression on the human mesangial cell cycle. Mol Med Rep. 11:910–916. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Tian J, Wang Y, Liu X, Zhou X and Li R: Rapamycin ameliorates IgA nephropathy via cell cycle-dependent mechanisms. Exp Biol Med (Maywood). 240:936–945. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Lu Y, Wen J, Chen D, Wu L, Li Q, Xie Y, Wu D, Liu X and Chen X: Modulation of cyclins and p53 in mesangial cell proliferation and apoptosis during Habu nephritis. Clin Exp Nephrol. 20:178–186. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Liu L, Gao C, Chen G, Li X, Li J, Wan Q and Xu Y: Notch signaling molecules activate TGF-β in rat mesangial cells under high glucose conditions. J Diabetes Res. 2013:9797022013. View Article : Google Scholar : PubMed/NCBI

27 

Toda N, Mukoyama M, Yanagita M and Yokoi H: CTGF in kidney fibrosis and glomerulonephritis. Inflamm Regen. 38:142018. View Article : Google Scholar : PubMed/NCBI

28 

Riser BL, Denichilo M, Cortes P, Baker C, Grondin JM, Yee J and Narins RG: Regulation of connective tissue growth factor activity in cultured rat mesangial cells and its expression in experimental diabetic glomerulosclerosis. J Am Soc Nephrol. 11:25–38. 2000.PubMed/NCBI

29 

Zhao WX, Zhuang X, Huang TT, Feng R and Lin JH: Effects of Notch2 and Notch3 on cell proliferation and apoptosis of trophoblast cell lines. Int J Med Sci. 12:867–874. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yu M, Guan Z, Li S, Wen X, Shi H, Qu G, Lu X, Zhu X, Wang B, Feng Q, Feng Q, et al: Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation. Mol Med Rep 20: 5297-5303, 2019.
APA
Yu, M., Guan, Z., Li, S., Wen, X., Shi, H., Qu, G. ... Zhang, A. (2019). Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation. Molecular Medicine Reports, 20, 5297-5303. https://doi.org/10.3892/mmr.2019.10753
MLA
Yu, M., Guan, Z., Li, S., Wen, X., Shi, H., Qu, G., Lu, X., Zhu, X., Wang, B., Feng, Q., Gan, W., Zhang, A."Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation". Molecular Medicine Reports 20.6 (2019): 5297-5303.
Chicago
Yu, M., Guan, Z., Li, S., Wen, X., Shi, H., Qu, G., Lu, X., Zhu, X., Wang, B., Feng, Q., Gan, W., Zhang, A."Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation". Molecular Medicine Reports 20, no. 6 (2019): 5297-5303. https://doi.org/10.3892/mmr.2019.10753
Copy and paste a formatted citation
x
Spandidos Publications style
Yu M, Guan Z, Li S, Wen X, Shi H, Qu G, Lu X, Zhu X, Wang B, Feng Q, Feng Q, et al: Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation. Mol Med Rep 20: 5297-5303, 2019.
APA
Yu, M., Guan, Z., Li, S., Wen, X., Shi, H., Qu, G. ... Zhang, A. (2019). Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation. Molecular Medicine Reports, 20, 5297-5303. https://doi.org/10.3892/mmr.2019.10753
MLA
Yu, M., Guan, Z., Li, S., Wen, X., Shi, H., Qu, G., Lu, X., Zhu, X., Wang, B., Feng, Q., Gan, W., Zhang, A."Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation". Molecular Medicine Reports 20.6 (2019): 5297-5303.
Chicago
Yu, M., Guan, Z., Li, S., Wen, X., Shi, H., Qu, G., Lu, X., Zhu, X., Wang, B., Feng, Q., Gan, W., Zhang, A."Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation". Molecular Medicine Reports 20, no. 6 (2019): 5297-5303. https://doi.org/10.3892/mmr.2019.10753
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team