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Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress

  • Authors:
    • Ronggang Li
    • Xin Li
    • Haibing Wu
    • Zhikun Yang
    • Li Fei
    • Jianhong Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Fudan University Huashan Hospital and State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Shanghai Medical College, Fudan University, Shanghai 200040, P.R. China, Department of Imaging, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, P.R. China, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P.R. China, Department of Neurosurgery, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4893-4904
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    Published online on: October 17, 2019
       https://doi.org/10.3892/mmr.2019.10755
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Abstract

Theaflavin has been proven to own strong antioxidative capacity; however, the molecular mechanism underlying its protective effect against cerebral ischemia‑reperfusion (I/R) injury remains unclear. Therefore, the present study was designed to elucidate the neuroprotective effects of theaflavin on cerebral I/R injury and its underlying molecular mechanisms. To investigate the effects of theaflavin on neurological function, neurogenesis, and oxidative stress, experiments were performed using a cerebral I/R injury rat model, and neural stem cells (NSCs) were subjected to oxygen‑glucose deprivation and reoxygenation (OGD/R). Further, the expression profiles of miRNA‑128‑3p and the regulatory function of nuclear factor (erythroid‑derived 2)‑related factor 2 (Nrf2) were evaluated in these models. We found that theaflavin treatment significantly reduced infarct volume and neuronal injury, and thus improved the impaired memory and learning ability. Furthermore, theaflavin treatment significantly enhanced the increase in NSC proliferation, reduction in the apoptotic rate and inhibition of oxidative stress. Mechanistically, theaflavin targeted miRNA‑128‑3p and further activated the Nrf2 pathway to reduce oxidative stress. In summary, theaflavin has a strong ability to attenuate cerebral I/R injury through miRNA‑128‑3p‑mediated recovery of the impaired antioxidant defense system, which suggests that it could be a potential drug candidate for ischemic stroke.
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Copy and paste a formatted citation
Spandidos Publications style
Li R, Li X, Wu H, Yang Z, Fei L and Zhu J: Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress. Mol Med Rep 20: 4893-4904, 2019.
APA
Li, R., Li, X., Wu, H., Yang, Z., Fei, L., & Zhu, J. (2019). Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress. Molecular Medicine Reports, 20, 4893-4904. https://doi.org/10.3892/mmr.2019.10755
MLA
Li, R., Li, X., Wu, H., Yang, Z., Fei, L., Zhu, J."Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress". Molecular Medicine Reports 20.6 (2019): 4893-4904.
Chicago
Li, R., Li, X., Wu, H., Yang, Z., Fei, L., Zhu, J."Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress". Molecular Medicine Reports 20, no. 6 (2019): 4893-4904. https://doi.org/10.3892/mmr.2019.10755
Copy and paste a formatted citation
x
Spandidos Publications style
Li R, Li X, Wu H, Yang Z, Fei L and Zhu J: Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress. Mol Med Rep 20: 4893-4904, 2019.
APA
Li, R., Li, X., Wu, H., Yang, Z., Fei, L., & Zhu, J. (2019). Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress. Molecular Medicine Reports, 20, 4893-4904. https://doi.org/10.3892/mmr.2019.10755
MLA
Li, R., Li, X., Wu, H., Yang, Z., Fei, L., Zhu, J."Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress". Molecular Medicine Reports 20.6 (2019): 4893-4904.
Chicago
Li, R., Li, X., Wu, H., Yang, Z., Fei, L., Zhu, J."Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA‑128‑3p‑mediated Nrf2 inhibition and reducing oxidative stress". Molecular Medicine Reports 20, no. 6 (2019): 4893-4904. https://doi.org/10.3892/mmr.2019.10755
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