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Article

A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells

  • Authors:
    • Junhe Zhang
    • Junli Zhao
    • Qinwen Mao
    • Haibin Xia
  • View Affiliations / Copyright

    Affiliations: Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P.R. China, Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
  • Pages: 5257-5264
    |
    Published online on: October 23, 2019
       https://doi.org/10.3892/mmr.2019.10767
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Abstract

Mesenchymal stromal cells (MSCs) hold broad therapeutic potential in various diseases, however, it is difficult to produce sufficient numbers of MSCs for clinical application, therefore, improved culture systems are required. The present study aimed to develop a novel method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells (hUCB‑MSCs). A sequential culture method was developed that uses two types of culture media to optimize the isolation and culture of hUCB‑MSCs. First, DMEM supplemented with mesenchymal stem cell growth supplement was used to improve the colony formation and primary culture success rates of hUCB‑MSCs. Then, after removing the heterogeneous cell population, ordinary DMEM was used from the fourth passage. This method obtained hUCB‑MSCs with high culture efficiency and at a greatly reduced cost. The optimal culture conditions were determined and the hUCB‑MSCs were phenotypically characterized after passaging. Taken together, this simple, efficient and economical method can produce a large number of high‑quality hUCB‑MSCs in <1 month, therefore facilitating the future clinical applications of hUCB‑MSCs.
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1 

Squillaro T, Peluso G and Galderisi U: Clinical trials with mesenchymal stem cells: An update. Cell Transplant. 25:829–848. 2016. View Article : Google Scholar : PubMed/NCBI

2 

de Wolf C, van de Bovenkamp M and Hoefnagel M: Regulatory perspective on in vitro potency assays for human mesenchymal stromal cells used in immunotherapy. Cytotherapy. 19:784–797. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Miura Y: Human bone marrow mesenchymal stromal/stem cells: Current clinical applications and potential for hematology. Int J Hematol. 103:122–128. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Wang HX, Li ZY and Guo ZK and Guo ZK: Easily-handled method to isolate mesenchymal stem cells from coagulated human bone marrow samples. World J Stem Cells. 7:1137–1144. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Francis SL, Duchi S, Onofrillo C, Di Bella C and Choong PFM: Adipose-derived mesenchymal stem cells in the use of cartilage tissue engineering: The need for a rapid isolation procedure. Stem Cells Int. 2018:89475482018. View Article : Google Scholar : PubMed/NCBI

6 

Bharti D, Shivakumar SB, Park JK, Ullah I, Subbarao RB, Park JS, Lee SL, Park BW and Rho GJ: Comparative analysis of human Wharton's jelly mesenchymal stem cells derived from different parts of the same umbilical cord. Cell Tissue Res. 372:51–65. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Pham PV, Vu NB, Pham VM, Truong NH, Pham TL, Dang LT, Nguyen TT, Bui AN and Phan NK: Good manufacturing practice-compliant isolation and culture of human umbilical cord blood-derived mesenchymal stem cells. J Transl Med. 12:562014. View Article : Google Scholar : PubMed/NCBI

8 

Rossignoli F, Caselli A, Grisendi G, Piccinno S, Burns JS, Murgia A, Veronesi E, Loschi P, Masini C, Conte P, et al: Isolation, characterization, and transduction of endometrial decidual tissue multipotent mesenchymal stromal/stem cells from menstrual blood. Biomed Res Int. 2013:9018212013. View Article : Google Scholar : PubMed/NCBI

9 

Vellasamy S, Sandrasaigaran P, Vidyadaran S, George E and Ramasamy R: Isolation and characterisation of mesenchymal stem cells derived from human placenta tissue. World J Stem Cells. 4:53–61. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Magatti M, Pianta S, Silini A and Parolini O: Isolation, culture, and phenotypic characterization of mesenchymal stromal cells from the amniotic membrane of the human term placenta. Methods Mol Biol. 1416:233–244. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TM, Hockema JJ, Woods EJ and Goebel WS: Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods. 14:149–156. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Marshall GP II, Laywell ED, Zheng T, Steindler DA and Scott EW: In vitro-derived ‘neural stem cells’ function as neural progenitors without the capacity for self-renewal. Stem Cells. 24:731–738. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Chung DJ, Choi CB, Lee SH, Kang EH, Lee JH, Hwang SH, Han H, Lee JH, Choe BY, Lee SY, et al: Intraarterially delivered human umbilical cord blood-derived mesenchymal stem cells in canine cerebral ischemia. J Neurosci Res. 87:3554–3567. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Lee M, Jeong SY, Ha J, Kim M, Jin HJ, Kwon SJ, Chang JW, Choi SJ, Oh W, Yang YS, et al: Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo. Biochem Biophys Res Commun. 446:983–989. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Nagamura-Inoue T and He H: Umbilical cord-derived mesenchymal stem cells: Their advantages and potential clinical utility. World J Stem Cells. 6:195–202. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Bieback K and Netsch P: Isolation, culture, and characterization of human umbilical cord blood-derived mesenchymal stromal cells. Methods Mol Biol. 1416:245–258. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Laitinen A, Lampinen M, Liedtke S, Kilpinen L, Kerkelä E, Sarkanen JR, Heinonen T, Kogler G and Laitinen S: The effects of culture conditions on the functionality of efficiently obtained mesenchymal stromal cells from human cord blood. Cytotherapy. 18:423–437. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Fujii S, Miura Y, Iwasa M, Yoshioka S, Fujishiro A, Sugino N, Kaneko H, Nakagawa Y, Hirai H, Takaori-Kondo A, et al: Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells. J Clin Exp Hematop. 57:1–8. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Bieback K, Kern S, Klüter H and Eichler H: Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood. Stem Cells. 22:625–634. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Fan X, Liu T, Liu Y, Ma X and Cui Z: Optimization of primary culture condition for mesenchymal stem cells derived from umbilical cord blood with factorial design. Biotechnol Prog. 25:499–507. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Chen L, Zhang ZG, Chen B, Liu XZ, Liu ZL, Liu HL, Li G, Su ZG, Wang JF and Hui GZ: Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem cells derived from human umbilical cord blood cells in vitro. Neural Regen Res. 6:972–977. 2011.

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

De Schauwer C, Meyer E, Cornillie P, De Vliegher S, van de Walle GR, Hoogewijs M, Declercq H, Govaere J, Demeyere K, Cornelissen M and Van Soom A: Optimization of the isolation, culture, and characterization of equine umbilical cord blood mesenchymal stromal cells. Tissue Eng Part C Methods. 17:1061–1070. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Erices A, Conget P and Minguell JJ: Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 109:235–242. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Manca MF, Zwart I, Beo J, Palasingham R, Jen LS, Navarrete R, Girdlestone J and Navarrete CV: Characterization of mesenchymal stromal cells derived from full-term umbilical cord blood. Cytotherapy. 10:54–68. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Ibrahim AM, Elgharabawi NM, Makhlouf MM and Ibrahim OY: Chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in vitro. Microsc Res Tech. 78:667–675. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Nielsen H: Isolation and functional activity of human blood monocytes at adherence to plastic surfaces: Comparison of different detachment methods. Acta Pathol Microbiol Immunol Scand C. 95:81–84. 1987.PubMed/NCBI

28 

Revencu T. Trifan V, Nacu L, Gutium T, Globa L, Motoc AG and Nacu V: Collection, isolation and characterization of the stem cells of umbilical cord blood. Rom J Morphol Embryol. 54:291–297. 2013.PubMed/NCBI

29 

Gang EJ, Hong SH, Jeong JA, Hwang SH, Kim SW, Yang IH, Ahn C, Han H and Kim H: In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells. Biochem Biophys Res Commun. 321:102–108. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Hsieh JY, Fu YS, Chang SJ, Tsuang YH and Wang HW: Functional module analysis reveals differential osteogenic and stemness potentials in human mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord. Stem Cells Dev. 19:1895–1910. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Oh W, Kim DS, Yang YS and Lee JK: Immunological properties of umbilical cord blood-derived mesenchymal stromal cells. Cell Immunol. 251:116–123. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Guo Y, Liu S, Wang P, Zhao S, Wang F, Bing L, Zhang Y, Ling EA, Gao J and Hao A: Expression profile of embryonic stem cell-associated genes Oct4, Sox2 and Nanog in human gliomas. Histopathology. 59:763–775. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Baksh D, Yao R and Tuan RS: Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells. 25:1384–1392. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Capasso S, Alessio N, Squillaro T, Di Bernardo G, Melone MA, Cipollaro M, Peluso G and Galderisi U: Changes in autophagy, proteasome activity and metabolism to determine a specific signature for acute and chronic senescent mesenchymal stromal cells. Oncotarget. 6:39457–39468. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Zhao J, Mao Q and Xia H: A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells. Mol Med Rep 20: 5257-5264, 2019.
APA
Zhang, J., Zhao, J., Mao, Q., & Xia, H. (2019). A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells. Molecular Medicine Reports, 20, 5257-5264. https://doi.org/10.3892/mmr.2019.10767
MLA
Zhang, J., Zhao, J., Mao, Q., Xia, H."A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells". Molecular Medicine Reports 20.6 (2019): 5257-5264.
Chicago
Zhang, J., Zhao, J., Mao, Q., Xia, H."A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells". Molecular Medicine Reports 20, no. 6 (2019): 5257-5264. https://doi.org/10.3892/mmr.2019.10767
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Zhao J, Mao Q and Xia H: A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells. Mol Med Rep 20: 5257-5264, 2019.
APA
Zhang, J., Zhao, J., Mao, Q., & Xia, H. (2019). A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells. Molecular Medicine Reports, 20, 5257-5264. https://doi.org/10.3892/mmr.2019.10767
MLA
Zhang, J., Zhao, J., Mao, Q., Xia, H."A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells". Molecular Medicine Reports 20.6 (2019): 5257-5264.
Chicago
Zhang, J., Zhao, J., Mao, Q., Xia, H."A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells". Molecular Medicine Reports 20, no. 6 (2019): 5257-5264. https://doi.org/10.3892/mmr.2019.10767
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