Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2019 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2019 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

  • Authors:
    • Huifeng Yang
    • Haocong Zhang
    • Yu Li
    • Liangbi Xiang
    • Jun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, General Hospital of Northern Theater Command, Shenyang, Liaoning 110015, P.R. China
  • Pages: 5265-5271
    |
    Published online on: October 30, 2019
       https://doi.org/10.3892/mmr.2019.10780
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Vitamin D deficiency may lead to an increased risk of tuberculosis. In the present study, the effects of Mycobacterium tuberculosis (Mtb) infection on dendritic cells (DCs) derived from vitamin D‑deficient mice or normal control mice were investigated. A vitamin D‑deficient mouse model was established, and bone marrow‑derived DCs (BMDCs) were isolated and treated with GM‑CSF and interleukin (IL)‑4 for 6 days, followed by an additional 24 h of treatment with Bacillus Calmette‑Guérin (BCG). The expression levels of surface molecules of DCs, including integrin alpha‑X and T‑lymphocyte activation antigen CD86, were significantly increased by BCG in the vitamin D‑deficient mice model group compared with the control group, while those of T‑lymphocyte activation antigen CD80, major histocompatibility complex class I and major histocompatibility complex class II were significantly decreased. These changes were BCG concentration‑dependent. In addition, the levels of IL‑4, IL‑6 and IL‑10 in the BMDCs from the vitamin D‑deficient mice were significantly decreased compared with the control mice, while the levels of tumor necrosis factor‑α, IL‑5, IL‑2, IL‑12 and interferon‑γ were significantly increased. Furthermore, the expression levels of vitamin D receptor (VDR) and CYP27B1 protein in the BMDCs from the vitamin D‑deficient mice were decreased compared with the control. BCG significantly increased the expression levels of VDR and CYP27B1 in the BMDCs. The DCs treated with BCG significantly induced the viability of CD4+ T lymphocytes. Therefore, BCG increases DCs and may enhance immunofunction, which may assist in preventing the risk of tuberculosis in patients with a vitamin D deficiency.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Flynn JL: Immunology of tuberculosis and implications in vaccine development. Tuberculosis (Edinb). 84:93–101. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Liu PT, Stenger S, Li H, Wenzel L, Tan BH, Krutzik SR, Ochoa MT, Schauber J, Wu K, Meinken C, et al: Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science. 311:1770–1773. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Talat N, Perry S, Parsonnet J, Dawood G and Hussain R: Vitamin D deficiency and tuberculosis progression. Emerg Infect Dis. 16:853–855. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zignol M, van Gemert W, Falzon D, Sismanidis C, Glaziou P, Floyd K and Raviglione M: Surveillance of anti-tuberculosis drug resistance in the world: An updated analysis, 2007–2010. Bull World Health Organ. 90:111–119D. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Goswami R, Mishra SK and Kochupillai N: Prevalence & potential significance of vitamin D deficiency in Asian Indians. Indian J Med Res. 127:229–238. 2008.PubMed/NCBI

6 

Holick MF: Vitamin D deficiency. N Engl J Med. 357:266–281. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Querfeld U: Vitamin D and inflammation. Pediatr Nephrol. 28:605–610. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Dusso AS, Brown AJ and Slatopolsky E: Vitamin D. Am J Physiol Renal Physiol. 289:F8–F28. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Pike JW and Meyer MB: The vitamin D receptor: New paradigms for the regulation of gene expression by 1,25-dihydroxyvitamin D3. Rheum Dis Clin North Am. 38:13–27. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Chen Y, Liu W, Sun T, Huang Y, Wang Y, Deb DK, Yoon D, Kong J, Thadhani R and Li YC: 1,25-Dihydroxyvitamin D promotes negative feedback regulation of TLR signaling via targeting microRNA-155-SOCS1 in macrophages. J Immunol. 190:3687–3695. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Von Essen MR, Kongsbak M, Schjerling P, Olgaard K, Ødum N and Geisler C: Vitamin D controls T cell antigen receptor signaling and activation of human T cells. Nat Immunol. 11:344–349. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Rook GA, Steele J, Fraher L, Barker S, Karmali R, O'Riordan J and Stanford J: Vitamin D3, gamma interferon, and control of proliferation of Mycobacterium tuberculosis by human monocytes. Immunology. 57:159–163. 1986.PubMed/NCBI

13 

Bikle DD: Vitamin D and the immune system: Role in protection against bacterial infection. Curr Opin Nephrol Hypertens. 17:348–352. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Veldman CM, Cantorna MT and DeLuca HF: Expression of 1,25-dihydroxyvitamin D (3) receptor in the immune system. Arch Biochem Biophys. 374:334–338. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Miziara ID, Magalhaes AT, Santos M, Gomes EF and Oliveira RA: Research ethics in animal models. Braz J Otorhinolaryngol (Article in English, Portuguese). 78:128–131. 2012. View Article : Google Scholar

16 

Wu SY, Chen WH, Hsieh CL and Lin YW: Abundant expression and functional participation of TRPV1 at Zusanli acupoint (ST36) in mice: Mechanosensitive TRPV1 as an ‘acupuncture-responding channel’. BMC Complement Altern Med. 14:962014. View Article : Google Scholar : PubMed/NCBI

17 

Liang Z, Xie Y, Dominguez JA, Breed ER, Yoseph BP, Burd EM, Farris AB, Davidson NO and Coopersmith CM: Intestine-specific deletion of microsomal triglyceride transfer protein increases mortality in aged mice. PLoS One. 9:e1018282014. View Article : Google Scholar : PubMed/NCBI

18 

https://www.nc3rs.org.uk/arrive-guidelines

19 

Zeng Y and Liu J: Role of glypican-1 in endothelial NOS activation under various steady shear stress magnitudes. Exp Cell Res. 348:184–189. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Zeng Y, Yao X, Liu X, He X, Li L, Liu X, Yan Z, Wu J and Fu BM: Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis. J Extracellular Vesicles. 8:16298652019. View Article : Google Scholar

21 

Gibney KB, MacGregor L, Leder K, Torresi J, Marshall C, Ebeling PR and Biggs BA: Vitamin D deficiency is associated with tuberculosis and latent tuberculosis infection in immigrants from Sub-Saharan Africa. Clin Infect Dis. 46:443–446. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Huang SJ, Wang XH, Liu ZD, Cao WL, Han Y, Ma AG and Xu SF: Vitamin D deficiency and the risk of tuberculosis: A meta-analysis. Drug Des Devel Ther. 11:91–102. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Penna G and Adorini L: 1 Alpha,25-dihydroxyvitamin D3 inhibits differentiation, maturation, activation, and survival of dendritic cells leading to impaired alloreactive T cell activation. J Immunol. 164:2405–2411. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Mosmann TR, Cherwinski H, Bond MW, Giedlin MA and Coffman RL: Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 136:2348–2357. 1986.PubMed/NCBI

25 

Lichtner M, Rossi R, Mengoni F, Vignoli S, Colacchia B, Massetti AP, Kamga I, Hosmalin A, Vullo V and Mastroianni CM: Circulating dendritic cells and interferon-alpha production in patients with tuberculosis: Correlation with clinical outcome and treatment response. Clin Exp Immunol. 143:329–337. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Dominguez J, De Souza-Galvao M, Ruiz-Manzano J, Latorre I, Prat C, Lacoma A, Milà C, Jiménez MA, Blanco S, Maldonado J, et al: T-cell responses to the mycobacterium tuberculosis-specific antigens in active tuberculosis patients at the beginning, during, and after antituberculosis treatment. Diagn Microbiol Infect Dis. 63:43–51. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Infante-Duarte C and Kamradt T: Th1/Th2 balance in infection. Springer Semin Immunopathol. 21:317–338. 1999. View Article : Google Scholar : PubMed/NCBI

28 

He XY, Xiao L, Chen HB, Hao J, Li J, Wang YJ, He K, Gao Y and Shi BY: T regulatory cells and Th1/Th2 cytokines in peripheral blood from tuberculosis patients. Eur J Clin Microbiol Infect Dis. 29:643–650. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Nemeth J, Winkler HM, Boeck L, Adegnika AA, Clement E, Mve TM, Kremsner PG and Winkler S: Specific cytokine patterns of pulmonary tuberculosis in Central Africa. Clin Immunol. 138:50–59. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Handzel ZT, Barak V, Altman Y, Bibi H, Lidgi M, Iancovici-Kidon M, Yassky D and Raz M: Increased Th1 and Th2 type cytokine production in patients with active tuberculosis. Isr Med Assoc J. 9:479–483. 2007.PubMed/NCBI

31 

Flynn JL, Goldstein MM, Triebold KJ, Sypek J, Wolf S and Bloom BR: IL-12 increases resistance of BALB/c mice to mycobacterium tuberculosis infection. J Immunol. 155:2515–2524. 1995.PubMed/NCBI

32 

Tissandié E, Guéguen Y, Lobaccaro JM, Aigueperse J and Souidi M: Vitamin D: Metabolism, regulation and associated diseases. Med Sci (Paris). 22:1095–1100. 2006.(In French). View Article : Google Scholar : PubMed/NCBI

33 

Nebbioso M, Buomprisco G, Pascarella A and Pescosolido N: Modulatory effects of 1,25-dihydroxyvitamin D3 on eye disorders: A critical review. Crit Rev Food Sci Nutr. 57:559–565. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Boonstra A, Barrat FJ, Crain C, Heath VL, Savelkoul HF and O'Garra A: 1α,25-Dihydroxyvitamin D3 has a direct effect on naive CD4+ T Cells to enhance the development of Th2 cells. J Immunol. 167:4974–4980. 2001. View Article : Google Scholar : PubMed/NCBI

35 

Gregori S, Casorati M, Amuchastegui S, Smiroldo S, Davalli AM and Adorini L: Regulatory T cells induced by 1 alpha,25-dihydroxyvitamin D3 and mycophenolate mofetil treatment mediate transplantation tolerance. J Immunol. 167:1945–1953. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang H, Zhang H, Li Y, Xiang L and Liu J: BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice. Mol Med Rep 20: 5265-5271, 2019.
APA
Yang, H., Zhang, H., Li, Y., Xiang, L., & Liu, J. (2019). BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice. Molecular Medicine Reports, 20, 5265-5271. https://doi.org/10.3892/mmr.2019.10780
MLA
Yang, H., Zhang, H., Li, Y., Xiang, L., Liu, J."BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice". Molecular Medicine Reports 20.6 (2019): 5265-5271.
Chicago
Yang, H., Zhang, H., Li, Y., Xiang, L., Liu, J."BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice". Molecular Medicine Reports 20, no. 6 (2019): 5265-5271. https://doi.org/10.3892/mmr.2019.10780
Copy and paste a formatted citation
x
Spandidos Publications style
Yang H, Zhang H, Li Y, Xiang L and Liu J: BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice. Mol Med Rep 20: 5265-5271, 2019.
APA
Yang, H., Zhang, H., Li, Y., Xiang, L., & Liu, J. (2019). BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice. Molecular Medicine Reports, 20, 5265-5271. https://doi.org/10.3892/mmr.2019.10780
MLA
Yang, H., Zhang, H., Li, Y., Xiang, L., Liu, J."BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice". Molecular Medicine Reports 20.6 (2019): 5265-5271.
Chicago
Yang, H., Zhang, H., Li, Y., Xiang, L., Liu, J."BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice". Molecular Medicine Reports 20, no. 6 (2019): 5265-5271. https://doi.org/10.3892/mmr.2019.10780
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team