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Article

IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine

  • Authors:
    • Sen Wang
    • Zhen Huang
    • Wenrui Li
    • Suhui He
    • Hongmei Wu
    • Jiandong Zhu
    • Rumeng Li
    • Zhuangyan Liang
    • Zhangquan Chen
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Longgang Central Hospital, Shenzhen, Guangdong 518116, P.R. China, Eighth People's Hospital of Dongguan City, Dongguan, Guangdong 523321, P.R. China
  • Pages: 371-378
    |
    Published online on: November 6, 2019
       https://doi.org/10.3892/mmr.2019.10804
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Abstract

As a novel anti‑inflammatory cytokine of the interleukin (IL)‑1 family, IL‑37 protects the human body from diseases characterized by excessive inflammation. The pathologic process of hyperhomocysteinemia (hHcy) is accompanied by persistent inflammation. However, little is known regarding the role of IL‑37 in hHcy. In the present study, the levels of cytokines including IL‑37, IL‑1β, IL‑6 and tumor necrosis factor‑α in the supernatant were detected by ELISA. mRNA and protein expression were detected by Reverse transcription‑quantitative PCR and western blotting, respectively. LDH level was determined by ELISA and the cell viability was detected through CCK‑8 kit. In the present study, mean serum IL‑37 levels of patients with hHcy were 32.3% lower than those of controls (P<0.01). In peripheral blood mononuclear cells (PBMCs) from patients with hHcy, mean IL‑37 mRNA expression was 73.5% lower (P<0.01) and IL‑37 protein expression was 77.7% lower compared with that of healthy controls (P<0.01). Furthermore, the results demonstrated that exogenous homocysteine (Hcy) stimulation markedly downregulated the mRNA and protein expression levels of IL‑37 in PBMCs in vitro. In 293T cells, overexpression of IL‑37 restored the cell viability impaired by Hcy, and reduced the release of lactate dehydrogenase and the proinflammatory cytokines IL‑1β, IL‑6 and tumor necrosis factor‑α. In conclusion, IL‑37 was downregulated by Hcy in vivo and in vitro, and IL‑37 exhibited a protective role against cell injury induced by Hcy.
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1 

Zhao J, Chen H, Liu N, Chen J, Gu Y, Chen J and Yang K: Role of hyperhomocysteinemia and hyperuricemia in pathogenesis of atherosclerosis. J Stroke Cerebrovasc Dis. 26:2695–2699. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Jakubowski H: Homocysteine modification in protein structure/function and human disease. Physiol Rev. 99:555–604. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Rong D, Liu J, Jia X, Al-Nafisee D, Jia S, Sun G, Li Y, Lu W, Li H, Zhang H, et al: Hyperhomocysteinaemia is an independent risk factor for peripheral arterial disease in a Chinese Han population. Atherosclerosis. 263:205–210. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Cao C, Hu J, Dong Y, Zhan R, Li P, Su H, Peng Q, Wu T, Lei L, Hang X, et al: Gender differences in the risk factors for endothelial dysfunction in Chinese hypertensive patients: Homocysteine is an independent risk factor in females. PLoS One. 10:e01186862015. View Article : Google Scholar : PubMed/NCBI

5 

Xie D, Yuan Y, Guo J, Yang S, Xu X, Wang Q, Li Y, Qin X, Tang G, Huo Y, et al: Hyperhomocysteinemia predicts renal function decline: A prospective study in hypertensive adults. Sci Rep. 5:162682015. View Article : Google Scholar : PubMed/NCBI

6 

Suszyńska-Zajczyk J, Utyro O and Jakubowski H: Methionine-induced hyperhomocysteinemia and bleomycin hydrolase deficiency alter the expression of mouse kidney proteins involved in renal disease. Mol Genet Metab. 112:339–346. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Petras M, Tatarkova Z, Kovalska M, Mokra D, Dobrota D, Lehotsky J and Drgova A: Hyperhomocysteinemia as a risk factor for the neuronal system disorders. J Physiol Pharmacol. 65:15–23. 2014.PubMed/NCBI

8 

Ashjazadeh N, Fathi M and Shariat A: Evaluation of homocysteine level as a risk factor among patients with ischemic stroke and its subtypes. Iran J Med Sci. 38:233–239. 2013.PubMed/NCBI

9 

Kamat PK, Vacek JC, Kalani A and Tyagi N: Homocysteine induced cerebrovascular dysfunction: A link to Alzheimer's disease etiology. Open Neurol J. 9:9–14. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Nazef K, Khelil M, Chelouti H, Kacimi G, Bendini M, Tazir M, Belarbi S, El Hadi Cherifi M and Djerdjouri B: Hyperhomocysteinemia is a risk factor for Alzheimer's disease in an Algerian population. Arch Med Res. 45:247–250. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Han L, Wu Q, Wang C, Hao Y, Zhao J, Zhang L, Fan R, Liu Y, Li R, Chen Z, et al: Homocysteine, ischemic stroke and coronary heart disease in hypertensive patients. Stroke. 46:1777–1786. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Durgaprasad R, Akkulagari V, Kasala L, Velam V and Sarma P: GW27-e0552 Novel single nucleotide polymorphisms identified in Exon-10 of Human coagulation factor V gene in patients with pulmonary embolism. J Am Coll Cardiol. 68:C48. 2016. View Article : Google Scholar

13 

Mazza A, Cicero AF, Ramazzina E, Lenti S, Schiavon L, Casiglia E and Gussoni G: Nutraceutical approaches to homocysteine lowering in hypertensive subjects at low cardiovascular risk: A multicenter, randomized clinical trial. J Biol Regul Homeost Agents. 30:921–927. 2016.PubMed/NCBI

14 

Wang CY, Chen ZW, Zhang T, Liu J, Chen SH, Liu SY, Han LY, Hui ZH and Chen YM: Elevated plasma homocysteine level is associated with ischemic stroke in Chinese hypertensive patients. Eur J Intern Med. 25:538–544. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Nold MF, Nold-Petry CA, Zepp JA, Palmer BE, Bufler P and Dinarello CA: IL-37 is a fundamental inhibitor of innate immunity. Nat Immunol. 11:1014–1022. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Boraschi D, Lucchesi D, Hainzl S, Leitner M, Maier E, Mangelberger D, Oostingh GJ, Pfaller T, Pixner C, Posselt G, et al: IL-37: A new anti-inflammatory cytokine of the IL-1 family. Eur Cytokine Netw. 22:127–147. 2011.PubMed/NCBI

17 

Luo Y, Cai X, Liu S, Wang S, Nold-Petry CA, Nold MF, Bufler P, Norris D, Dinarello CA and Fujita M: Suppression of antigen-specific adaptive immunity by IL-37 via induction of tolerogenic dendritic cells. Proc Natl Acad Sci USA. 111:15178–15183. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Li Y, Wang Y, Liu Y, Wang Y, Zuo X, Li Y and Lu X: The possible role of the novel cytokines il-35 and il-37 in inflammatory bowel disease. Mediators Inflamm. 2014:1363292014. View Article : Google Scholar : PubMed/NCBI

19 

Ye L, Jiang B, Deng J, Du J, Xiong W, Guan Y, Wen Z, Huang K and Huang Z: IL-37 Alleviates rheumatoid arthritis by suppressing IL-17 and IL-17-triggering cytokine production and limiting Th17 cell proliferation. J Immunol. 194:5110–5119. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Zhao PW, Jiang WG, Wang L, Jiang ZY, Shan YX and Jiang YF: Plasma levels of IL-37 and correlation with TNF-α, IL-17A, and disease activity during DMARD treatment of rheumatoid arthritis. PLoS One. 9:e953462014. View Article : Google Scholar : PubMed/NCBI

21 

Ye L, Ji L, Wen Z, Zhou Y, Hu D, Li Y, Yu T, Chen B, Zhang J, Ding L, et al: IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus: Its correlation with disease activity. J Transl Med. 12:692014. View Article : Google Scholar : PubMed/NCBI

22 

Conti P, Lessiani G, Kritas SK, Ronconi G, Caraffa A and Theoharides TC: Mast cells emerge as mediators of atherosclerosis: Special emphasis on IL-37 inhibition. Tissue Cell. 49:393–400. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Ji Q, Zeng Q, Huang H, Shi Y, Lin Y, Lu Z, Meng K, Wu B, Yu K, Chai M, et al: Elevated plasma IL-37, IL-18, and IL-18BP concentrations in patients with acute coronary syndrome. Mediat Inflamm. 2014:1657422014. View Article : Google Scholar

24 

Wu B, Meng K, Ji Q, Cheng M, Yu K, Zhao X, Tony H, Liu Y, Zhou Y, Chang C, et al: Interleukin-37 ameliorates myocardial ischaemia/reperfusion injury in mice. Clin Exp Immunol. 176:438–451. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Ye Z, Wang C, Tang J, Zhou Y, Bai L, Liu Y, Kijlstra A and Yang P: Decreased interleukin-37 expression in Vogt-Koyanagi-Harada disease and upregulation following immunosuppressive treatment. J Interferon Cytokine Res. 35:265–272. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Asanuma Y, Oeser A, Stanley E, Shintani A and Stein CM: The effect of homocysteine on the production of inflammatory cytokines. Clin Pharmacol Ther. 75:P492004. View Article : Google Scholar

28 

Zanin RF, Bergamin LS, Morrone FB, Coutinho-Silva R, de Souza Wyse AT and Battastini AM: Pathological concentrations of homocysteine increases IL-1β production in macrophages in a P2X7, NF-ĸB, and erk-dependent manner. Purinergic Signal. 11:463–470. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Han S, Wu H, Li W and Gao P: Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury. Mol Cell Biochem. 403:43–49. 2015. View Article : Google Scholar : PubMed/NCBI

30 

McNamee EN, Masterson JC, Jedlicka P, McManus M, Grenz A, Collins CB, Nold MF, Nold-Petry C, Bufler P, Dinarello CA and Rivera-Nieves J: Interleukin 37 expression protects mice from colitis. Proc Natl Acda Sci USA. 108:16711–16716. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Huang Z, Li W, He S, Wu H, Zhu J, Li R, Liang Z and Chen Z: IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine. Mol Med Rep 21: 371-378, 2020.
APA
Wang, S., Huang, Z., Li, W., He, S., Wu, H., Zhu, J. ... Chen, Z. (2020). IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine. Molecular Medicine Reports, 21, 371-378. https://doi.org/10.3892/mmr.2019.10804
MLA
Wang, S., Huang, Z., Li, W., He, S., Wu, H., Zhu, J., Li, R., Liang, Z., Chen, Z."IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine". Molecular Medicine Reports 21.1 (2020): 371-378.
Chicago
Wang, S., Huang, Z., Li, W., He, S., Wu, H., Zhu, J., Li, R., Liang, Z., Chen, Z."IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine". Molecular Medicine Reports 21, no. 1 (2020): 371-378. https://doi.org/10.3892/mmr.2019.10804
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Huang Z, Li W, He S, Wu H, Zhu J, Li R, Liang Z and Chen Z: IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine. Mol Med Rep 21: 371-378, 2020.
APA
Wang, S., Huang, Z., Li, W., He, S., Wu, H., Zhu, J. ... Chen, Z. (2020). IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine. Molecular Medicine Reports, 21, 371-378. https://doi.org/10.3892/mmr.2019.10804
MLA
Wang, S., Huang, Z., Li, W., He, S., Wu, H., Zhu, J., Li, R., Liang, Z., Chen, Z."IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine". Molecular Medicine Reports 21.1 (2020): 371-378.
Chicago
Wang, S., Huang, Z., Li, W., He, S., Wu, H., Zhu, J., Li, R., Liang, Z., Chen, Z."IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine". Molecular Medicine Reports 21, no. 1 (2020): 371-378. https://doi.org/10.3892/mmr.2019.10804
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